公开(公告)号：US11613773B2

公开(公告)日：2023-03-28

申请号：US17834474

申请日：2022-06-07

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12N15/10

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20240068016A1

公开(公告)日：2024-02-29

申请号：US18505624

申请日：2023-11-09

申请人： 10x Genomics Sweden AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12N15/10 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876

CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20160017396A1

公开(公告)日：2016-01-21

申请号：US14804068

申请日：2015-07-20

申请人： ILLUMINA, INC.

发明人： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C12N15/102 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2563/131

摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

摘要翻译： 一种富含靶核酸的方法，包括提供具有crRNA或其衍生物的内切核酸酶系统和Cas蛋白质或其变体。 所述crRNA或其衍生物含有与靶核酸区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物，从而富集靶核酸。

公开(公告)号：US11162132B2

公开(公告)日：2021-11-02

申请号：US17237670

申请日：2021-04-22

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6874

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20230183785A1

公开(公告)日：2023-06-15

申请号：US18166853

申请日：2023-02-09

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6876

CPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6876

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20220195505A1

公开(公告)日：2022-06-23

申请号：US17693116

申请日：2022-03-11

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6876

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20220002791A1

公开(公告)日：2022-01-06

申请号：US17479718

申请日：2021-09-20

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6876

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20210292748A1

公开(公告)日：2021-09-23

申请号：US17237652

申请日：2021-04-22

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12N15/10

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20200165650A1

公开(公告)日：2020-05-28

申请号：US16659475

申请日：2019-10-21

申请人： Illumina, Inc.

发明人： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC分类号： C12P19/34 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q1/6816 ,  C12N15/10

摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

公开(公告)号：US20230304078A1

公开(公告)日：2023-09-28

申请号：US18314437

申请日：2023-05-09

申请人： Spatial Transcriptomics AB ,  Illumina, Inc.

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6876

CPC分类号： C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6876

摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.