公开(公告)号：US20090162835A9

公开(公告)日：2009-06-25

申请号：US10045674

申请日：2001-10-25

Applicant: Robert Ladner ,  Edward Cohen ,  Horacio Nastri ,  Kristin Rookey ,  Rene Hoet ,  Hendricus Mattheus Hoogenboom

Inventor： Robert Ladner ,  Edward Cohen ,  Horacio Nastri ,  Kristin Rookey ,  Rene Hoet ,  Hendricus Mattheus Hoogenboom

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C07K16/005 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55 ,  C07K2317/622 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/1093 ,  C12N15/66 ,  C40B40/02 ,  C40B40/08

Abstract: Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.

Abstract translation: 用于构建共同显示和/或表达肽，多肽或蛋白质不同家族成员的文库的方法和使用这些方法产生的文库。 筛选这些文库和通过这种筛选鉴定的肽，多肽或蛋白质的方法。

公开(公告)号：US20070148774A1

公开(公告)日：2007-06-28

申请号：US11555519

申请日：2006-11-01

Applicant: John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

Inventor： John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

IPC: C12N15/86 ,  C12N15/74

CPC classification number: C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US20080108514A1

公开(公告)日：2008-05-08

申请号：US11862791

申请日：2007-09-27

Applicant: Hendricus Mattheus Hoogenboom

Inventor： Hendricus Mattheus Hoogenboom

IPC: C40B40/08

CPC classification number: C07K16/26 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/55 ,  C40B50/06

Abstract: The present invention provides Fab libraries and methods for using the Fab libraries to obtain antibodies against a target. The Fab library of the invention contains at least 109 different Fabs, and in some embodiments, at least 1010 different Fabs. The Fab libraries of the invention are used to isolate polyclonal or monoclonal Fabs that bind with high specificity to targets.

Abstract translation: 本发明提供Fab文库和使用Fab文库获得针对靶抗体的方法。 本发明的Fab文库含有至少10个不同的Fab，在一些实施方案中包含至少10个不同的Fab。 本发明的Fab文库用于分离以高特异性结合靶标的多克隆或单克隆Fab。

公开(公告)号：US20060160184A1

公开(公告)日：2006-07-20

申请号：US11292414

申请日：2005-11-30

Applicant: Hendricus Mattheus Hoogenboom ,  Ton Logtenberg

Inventor： Hendricus Mattheus Hoogenboom ,  Ton Logtenberg

IPC: C12Q1/68 ,  C12P21/06

CPC classification number: G01N33/56966 ,  C07K16/005 ,  C07K16/10 ,  C07K16/22 ,  C07K16/248 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

Abstract: The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

Abstract translation: 本发明提供了被设计为真实组合的特异性结合蛋白（例如免疫球蛋白）的组合，该组合的基本上所有组分都是功能性的并且彼此相容。 本发明进一步提供一种制备包含至少两种不同蛋白质分子的组合物的方法，所述蛋白质分子包含配对可变区，所述至少两种具有不同结合特异性的蛋白质分子包含配对的可变区，至少两种具有不同结合特异性的蛋白质分子，包括接触 至少三个不同的可变区，在允许可变区配对和收获基本上所有具有由配对产生的结合特异性的蛋白质分子的条件下。