公开(公告)号：US20070148774A1

公开(公告)日：2007-06-28

申请号：US11555519

申请日：2006-11-01

Applicant: John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

Inventor： John McCafferty ,  Anthony Pope ,  Kevin Johnson ,  Hendricus Mattheus Hoogenboom ,  Andrew Griffiths ,  Ronald Jackson ,  Kasper Holliger ,  James Marks ,  Timothy Clackson ,  David Chiswell ,  Gregory Winter ,  Timothy Bonnert

IPC: C12N15/86 ,  C12N15/74

CPC classification number: C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US20060019260A1

公开(公告)日：2006-01-26

申请号：US10910463

申请日：2004-08-02

Applicant: Richard Lerner ,  Joseph Sorge ,  Gregory Winter ,  Lutz Riechmann

Inventor： Richard Lerner ,  Joseph Sorge ,  Gregory Winter ,  Lutz Riechmann

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C07K16/005 ,  C07K16/00 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/569 ,  C07K2317/622 ,  C12Q1/6876

Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.

Abstract translation: 本发明涉及从免疫基因库中分离编码具有结合预选配体的能力的受体的基因的方法。 还考虑了通过该方法分离的基因产生的受体，特别是催化受体。

公开(公告)号：US20080050359A1

公开(公告)日：2008-02-28

申请号：US11625750

申请日：2007-01-22

Applicant: Andrew Griffiths ,  Hendricus Renerus Jacobus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

Inventor： Andrew Griffiths ,  Hendricus Renerus Jacobus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

IPC: A61K31/395 ,  A61P43/00 ,  C07K16/00 ,  C40B20/08

CPC classification number: C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

Abstract translation: 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所用的核酸文库可能来源于未免疫哺乳动物的重排的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US20070232790A1

公开(公告)日：2007-10-04

申请号：US11625769

申请日：2007-01-22

Applicant: ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

Inventor： ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

IPC: C07K16/18

CPC classification number: C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

Abstract translation: 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所用的核酸文库可能来源于未免疫哺乳动物的重排的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US20060083747A1

公开(公告)日：2006-04-20

申请号：US11166496

申请日：2005-06-24

Applicant: Gregory Winter ,  Ian Tomlinson ,  Olga Ignatovich ,  Neil Brewis

Inventor： Gregory Winter ,  Ian Tomlinson ,  Olga Ignatovich ,  Neil Brewis

IPC: A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06 ,  C07K16/46

CPC classification number: C07K16/00 ,  A61K2039/505 ,  C07K16/241 ,  C07K16/40 ,  C07K2317/52 ,  C07K2317/569 ,  C07K2317/94 ,  C07K2319/00

Abstract: The present invention relates to a simple method for generating antibody-based structures suitable for in vivo use. In particular, the invention relates to a method for the generation of antibody-based structures suitable for in vivo use comprising the steps of: (a) selecting an antibody single variable domain having an epitope binding specificity; and (b) attaching the single domain of step (a) to an effector group. Uses of molecules generated using the method of the Invention are also described.

Abstract translation: 本发明涉及一种生成适用于体内使用的基于抗体的结构的简单方法。 特别地，本发明涉及一种生成适合于体内应用的基于抗体的结构的方法，包括以下步骤：（a）选择具有表位结合特异性的抗体单可变结构域; 和（b）将步骤（a）的单一结构域连接到效应子组。 还描述了使用本发明的方法生成的分子的用途。

公开(公告)号：US20050133015A1

公开(公告)日：2005-06-23

申请号：US11017029

申请日：2004-12-18

Applicant: Thomas Winter ,  Gregory Winter

Inventor： Thomas Winter ,  Gregory Winter

IPC: A63B69/40 ,  B62B1/00 ,  B62B1/10 ,  B62B5/00 ,  B62B5/06 ,  F41B4/00

CPC classification number: B62B1/00 ,  A63B69/40 ,  B62B1/10 ,  B62B5/0026 ,  B62B5/062 ,  B62B5/067 ,  B62B2301/252

Abstract: The present invention comprises a platform on which a ball machine is temporarily mounted and can be secured. Beneath the platform a type of pivot or other variety of mobility providing device(s) is/are attached, to allow the platform to move about in multiple directions and/or rotate. The support platform can be allowed to roll, rotate, tilt and turn while a ball machine is attached to it. Handle(s) can be attached to the platform to allow someone to manually manage/dictate the aim or position of the apparatus, thus control or change the direction of each ball fed when a ball machine is in operation and attached to the structure.

Abstract translation: 本发明包括一个平台，球机临时安装在该平台上并可固定。 在平台下方，连接有一种枢轴或其他各种移动性提供装置，以允许平台在多个方向上移动和/或旋转。 当球机连接到其上时，可以允许支撑平台滚动，旋转，倾斜和转动。 手柄可以附接到平台上，以允许某人手动地管理/指示设备的目的或位置，从而控制或改变当球机运行并附接到结构时馈送的每个球的方向。

公开(公告)号：US20070207475A1

公开(公告)日：2007-09-06

申请号：US11560769

申请日：2006-11-16

Applicant: William Huse ,  Gregory Winter ,  Lutz Riechmann ,  Joseph Sorge ,  Richard Lerner

Inventor： William Huse ,  Gregory Winter ,  Lutz Riechmann ,  Joseph Sorge ,  Richard Lerner

IPC: C12Q1/68 ,  C07K16/00

CPC classification number: C07K16/005 ,  A61K38/00 ,  C07K16/00 ,  C07K16/44 ,  C07K16/468 ,  C07K2317/55 ,  C07K2317/56

Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.

Abstract translation: 本发明涉及从免疫基因库中分离编码具有结合预选配体的能力的受体的基因的方法。 还考虑了通过该方法分离的基因产生的受体，特别是催化受体。

公开(公告)号：US20060280734A1

公开(公告)日：2006-12-14

申请号：US11165672

申请日：2005-06-24

Applicant: Gregory Winter ,  Ian Tomlinson ,  Olga Ignatovich

Inventor： Gregory Winter ,  Ian Tomlinson ,  Olga Ignatovich

IPC: A61K39/395 ,  C07K16/44 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC classification number: C07K16/005

Abstract: The present invention relates to a method for the generation of immunoglobulin molecules of predetermined specificity. In particular, the invention relates to a method for the retargeting of the epitope binding specificity of one or more antibodies using single variable domains which exhibit a dominant epitope binding specificity.

Abstract translation: 本发明涉及产生具有预定特异性的免疫球蛋白分子的方法。 特别地，本发明涉及使用显示优势表位结合特异性的单个可变结构域重定向一种或多种抗体的表位结合特异性的方法。

公开(公告)号：US20060111955A1

公开(公告)日：2006-05-25

申请号：US11287133

申请日：2005-11-23

Applicant: Gregory Winter ,  Daniel Huber ,  Naeem Bari

Inventor： Gregory Winter ,  Daniel Huber ,  Naeem Bari

IPC: G06F9/46

CPC classification number: H04M3/42 ,  G06Q10/06 ,  G06Q10/109 ,  G06Q10/1095 ,  G06Q10/1097 ,  G06Q50/30 ,  H04M2203/2072 ,  H04M2242/30 ,  H04W4/029

Abstract: A customer appointment management system and method with mobile resource management including a customer communication module configured for transmitting to a customer a notification of a pending arrival of a mobile agent at the customer location for an appointment and receiving a confirmation from the customer confirming the appointment. The system also includes a mobile agent communication module configured for transmitting a schedule and customer appointment information to the mobile agent in response to receiving the customer confirmation.

Abstract translation: 一种具有移动资源管理的客户预约管理系统和方法，包括客户通信模块，客户通信模块被配置为向客户发送移动代理等待到达客户位置处的预约的通知，并从客户接收确认该约定的确认。 该系统还包括移动代理通信模块，该移动代理通信模块被配置为响应于接收到客户确认而向移动代理发送调度和客户约定信息。

公开(公告)号：US20060111089A1

公开(公告)日：2006-05-25

申请号：US11287132

申请日：2005-11-23

Applicant: Gregory Winter ,  Daniel Huber ,  Naeem Bari

Inventor： Gregory Winter ,  Daniel Huber ,  Naeem Bari

IPC: H04Q7/38

CPC classification number: H04M3/42 ,  G06Q10/06 ,  G06Q10/109 ,  G06Q10/1095 ,  G06Q10/1097 ,  G06Q50/30 ,  H04M2203/2072 ,  H04M2242/30 ,  H04W4/029

Abstract: A mobile resource management system and method for managing the arrival of a mobile agent at a location of a customer, the system including a mobile agent location module, a customer communication module, and a mobile agent communication module. The mobile agent location module is configured for identifying a location of a mobile agent and the customer communication module configured for transmitting a notification to a customer of an availability of the mobile agent for arrival of the mobile agent at a location of the customer as a function, at least in part, of the identified mobile agent location, prompting the customer to confirm the arrival of the mobile agent at the customer location, and receiving a confirmation from the customer in response to the prompting. The mobile agent communication module is configured for transmitting schedule and customer appointment information to the mobile agent in response to receiving the customer confirmation.

Abstract translation: 一种用于管理移动代理到达客户位置的移动资源管理系统和方法，该系统包括移动代理位置模块，客户通信模块和移动代理通信模块。 移动代理位置模块被配置用于识别移动代理的位置，以及客户通信模块，该客户通信模块被配置为向客户发送用于移动代理到达的移动代理的可用性的通知作为功能的客户的位置 至少部分地被识别的移动代理位置，促使客户确认移动代理人到达客户位置，并响应于提示从客户接收确认。 移动代理通信模块被配置为响应于接收到客户确认而向移动代理发送时间表和客户约定信息。