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公开(公告)号：US20170159003A1
公开(公告)日：2017-06-08
申请号：US15327208
申请日：2015-07-08
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Sadamitsu ASO , Eiichiro TAKADA , Masako KAWARAI , Toshinari SAKURAI
CPC classification number: C12M41/40 , C12M23/58 , C12M27/00 , C12M29/14 , C12M29/18 , C12M31/10 , C12M41/06 , C12M41/36 , C12M41/48 , C12Q1/06
Abstract: A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.
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公开(公告)号：US20200225256A1
公开(公告)日：2020-07-16
申请号：US16330423
申请日：2017-08-04
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Masashi FUKAYA , Eiichiro TAKADA , Takuya TAKAHASHI
IPC: G01N35/10
Abstract: A cleaning water flow velocity during nozzle cleaning is momentarily changed by using a pressure changing mechanism, for example, a syringe. That is, after the start of cleaning, a solenoid valve is opened, and an aspirating operation of the syringe is momentarily performed by a fixed amount in a state where a flow of cleaning water in a nozzle is developed, whereby a nozzle flow velocity is momentarily decelerated. Immediately thereafter, the syringe is pushed back to a home position again, and the flow velocity in the nozzle is accelerated again. A cleaning effect can be improved by performing transition of velocity distribution to various states. Flow velocity control by the pressure changing mechanism can be realized without inhibiting a dispensing cycle, and the cleaning effect of the nozzle can be improved without changing the pressure of a liquid feeding pump or extending the cleaning time.
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公开(公告)号：US20180080002A1
公开(公告)日：2018-03-22
申请号：US15326938
申请日：2015-07-08
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Sadamitsu ASO , Eiichiro TAKADA , Masako KAWARAI , Toshinari SAKURAI
CPC classification number: C12M41/48 , C12M1/00 , C12M1/02 , C12M1/34 , C12M29/00 , C12M41/00 , C12M41/36 , C12M45/02 , C12M47/04
Abstract: The purpose of the present invention is to provide a means for dispersing cell aggregates without damaging the cells, such that a sufficient multiplication rate can be obtained in a subculture. According to the present invention, provided is a cell-suspension processing device which disperses cell aggregates included in a cell suspension. The device is provided with: an inlet for taking in the cell suspension; an outlet for discharging the processed cell suspension; and a flow path which is provided between the inlet and the outlet, and which is capable of holding the cell suspension. The flow path has, provided thereto, a liquid delivery pump for causing the cell suspension inside to flow, a cell-dispersion-degree measurement instrument for measuring the dispersion degree of cells in the cell suspension, and a narrow part for imparting shearing force to the cell suspension flowing inside. The cell-suspension processing device is further provided with a control unit for controlling at least the liquid delivery pump on the basis of data obtained by the cell-dispersion-degree measurement instrument. The control unit determines whether the cells have attained a prescribed dispersion degree on the basis of the data obtained by the cell-dispersion-degree measurement instrument, and, in cases when the cells have not attained the prescribed dispersion degree, drives the liquid delivery pump such that the cell suspension is passed through the narrow part.
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Patent Agency Ranking
公开(公告)号：US20170037351A1
公开(公告)日：2017-02-09
申请号：US15106945
申请日：2014-12-24
Applicant: Hitachi High-Technologies Corporation
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Eiichiro TAKADA , Sadamitsu ASO
IPC: C12M1/00
Abstract: According to the present invention, a simple structure can be used to achieve reliable liquid delivery with no residual air, and simple attachment/detachment of a culture vessel, and thus a closed-system cell culture device exhibiting high reliability can be constructed at low cost. In the present invention, a liquid is supplied or discharged while a culture vessel is in an inclined state. The culture vessel is provided with two flow paths, namely an intake flow path and a discharge flow path, which connect a culture chamber and a connection member. Points where the flow paths join with the culture chamber are respectively configured as an intake port and a discharge port. The discharge port is provided in the position nearest to the axis of inclination of the culture vessel. The intake port is provided in a plane projected from a vertical plane including the aforementioned axis of inclination.
Abstract translation: 根据本发明，可以使用简单的结构来实现无残留空气的可靠的液体输送，以及培养容器的简单的附着/分离，从而可以以低成本构建出具有高可靠性的封闭系统的细胞培养装置 。 在本发明中，在培养容器处于倾斜状态时供给或排出液体。 培养容器具有连接培养室和连接部件的两个流路，即进气流路和排出流路。 流路与培养室连接的点分别构成进气口和排出口。 排出口设置在最靠近培养容器倾斜轴线的位置。 进气口设置在从包括上述倾斜轴线的垂直平面投影的平面中。
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公开(公告)号：US20160319233A1
公开(公告)日：2016-11-03
申请号：US15107548
申请日：2014-12-24
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Eiichiro TAKADA , Sadamitsu ASO
CPC classification number: C12M23/40 , B01L3/502738 , B01L3/567 , B01L2200/025 , B01L2200/0668 , B01L2300/0816 , B01L2300/0838 , B01L2300/0861 , B01L2400/0487 , B01L2400/0622 , B01L2400/0655 , C12M23/14 , C12M23/58 , C12M29/00 , F16K7/045
Abstract: According to the present invention, a problem of closed systems, namely minimizing the number of electromagnetic valves required to control a plurality of flow paths, can be addressed, and thus a low-cost cell culture device can be achieved. In this flow-path control method for X number of flow paths satisfying X≦2N, the X number of flow paths are selected by using N number of valves to simultaneously and selectively control the opening and closing of the plurality of flow paths.
Abstract translation: 根据本发明，可以解决封闭系统的问题，即最小化控制多个流路所需的电磁阀的数量，从而可以实现低成本的细胞培养装置。 在该X-2N流路的流路控制方法中，通过使用N个阀来选择X个流路，以同时选择性地控制多个流路的开闭。
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公开(公告)号：US20190219604A1
公开(公告)日：2019-07-18
申请号：US16331583
申请日：2017-08-04
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Takuya TAKAHASHI , Hitoshi TOKIEDA , Eiichiro TAKADA , Masashi FUKAYA
CPC classification number: G01N35/00584 , G01N35/04 , G01N35/10 , G01N35/1004 , G01N35/1065 , G01N35/109 , G01N2035/00534 , G01N2035/0406 , G01N2035/0443 , G01N2035/0453
Abstract: An automatic analyzer capable of controlling an interval between the tip of a sample nozzle and the bottom of a reaction container regardless of individual differences between reaction containers and sample nozzles and suppressing adhesion of a sample to the sample nozzle is disclosed. Sample nozzles 13a and 14a are moved toward the bottom surface of a reaction container 2, the movement of the sample nozzles is stopped at a point in time when a stop position detector 46 detects a stop position detection plate 45, the sample nozzles are ascended from the stop position to a position where the stop position detection plate 45 separates from a detection range of the stop position detector 46, and an arm 44 is moved upward by a moving distance stored in a memory.
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公开(公告)号：US20190153377A1
公开(公告)日：2019-05-23
申请号：US15521348
申请日：2014-11-07
Applicant: Hitachi High-Technologies Corporation
Inventor： Akihiro SHIMASE , Eiichiro TAKADA , Kazumichi IMAI , Toshinari SAKURAI
Abstract: A sterile connector has a first connector including a first housing provided with a first flow channel; a first pipeline connected to the first flow channel; first and second openings, the first opening being positioned inward from the second opening; and a first sealing member covering the second opening. Also, a second connector includes a second housing provided with a second flow channel; a third opening; and a second sealing member covering the third opening. The first and second connectors are detachable from each other. The first sealing member seals a space between the inner circumferential surface of the first housing and the outer circumferential surface of the second housing, while the second sealing member seals a space between the inner circumferential surface of the second housing and the outer circumferential surface of the first pipeline so that the first flow channel communicates with the second flow channel.
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公开(公告)号：US20170191019A1
公开(公告)日：2017-07-06
申请号：US15320369
申请日：2015-07-08
Applicant: Hitachi High-Technologies Corporation
Inventor： Masako KAWARAI , Akihiro SHIMASE , Sadamitsu ASO , Toshinari SAKURAI , Eiichiro TAKADA , Kazumichi IMAI
CPC classification number: C12M33/00 , C12M29/00 , C12M41/00 , C12M41/36 , C12M41/48 , C12Q1/02 , G01N15/06 , G01N21/47 , G01N21/51 , G01N21/53 , G01N21/59 , G01N2015/0053 , G01N2015/0065 , G01N2015/0693 , G01N2201/12
Abstract: The present invention addresses the problem of providing: a cell dispersion measurement mechanism whereby it becomes possible to fully disperse cells regardless of the experiences of operators skilled in cell culture and it also becomes possible to determine the number or concentration of cells accurately; a cell culture apparatus equipped with the cell dispersion measurement mechanism; and a cell dispersion measurement method. The problem can be solved by circulating a cell suspension in a flow path to disperse cell masses contained in the cell suspension, and then determining over time the number or concentration of cells and/or the degree of dispersion of cells in the cell suspension that is flowing in the circulation flow path.
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