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公开(公告)号：US20170191019A1
公开(公告)日：2017-07-06
申请号：US15320369
申请日：2015-07-08
Applicant: Hitachi High-Technologies Corporation
Inventor： Masako KAWARAI , Akihiro SHIMASE , Sadamitsu ASO , Toshinari SAKURAI , Eiichiro TAKADA , Kazumichi IMAI
CPC classification number: C12M33/00 , C12M29/00 , C12M41/00 , C12M41/36 , C12M41/48 , C12Q1/02 , G01N15/06 , G01N21/47 , G01N21/51 , G01N21/53 , G01N21/59 , G01N2015/0053 , G01N2015/0065 , G01N2015/0693 , G01N2201/12
Abstract: The present invention addresses the problem of providing: a cell dispersion measurement mechanism whereby it becomes possible to fully disperse cells regardless of the experiences of operators skilled in cell culture and it also becomes possible to determine the number or concentration of cells accurately; a cell culture apparatus equipped with the cell dispersion measurement mechanism; and a cell dispersion measurement method. The problem can be solved by circulating a cell suspension in a flow path to disperse cell masses contained in the cell suspension, and then determining over time the number or concentration of cells and/or the degree of dispersion of cells in the cell suspension that is flowing in the circulation flow path.
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公开(公告)号：US20170306287A1
公开(公告)日：2017-10-26
申请号：US15518041
申请日：2014-10-22
Applicant: Hitachi High-Technologies Corporation
Inventor： Masako KAWARAI , Toshinari SAKURAI , Akihiro SHIMASE , Hiroyuki KOSHI
Abstract: A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.
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公开(公告)号：US20170159003A1
公开(公告)日：2017-06-08
申请号：US15327208
申请日：2015-07-08
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Sadamitsu ASO , Eiichiro TAKADA , Masako KAWARAI , Toshinari SAKURAI
CPC classification number: C12M41/40 , C12M23/58 , C12M27/00 , C12M29/14 , C12M29/18 , C12M31/10 , C12M41/06 , C12M41/36 , C12M41/48 , C12Q1/06
Abstract: A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.
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公开(公告)号：US20180080002A1
公开(公告)日：2018-03-22
申请号：US15326938
申请日：2015-07-08
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Akihiro SHIMASE , Kazumichi IMAI , Sadamitsu ASO , Eiichiro TAKADA , Masako KAWARAI , Toshinari SAKURAI
CPC classification number: C12M41/48 , C12M1/00 , C12M1/02 , C12M1/34 , C12M29/00 , C12M41/00 , C12M41/36 , C12M45/02 , C12M47/04
Abstract: The purpose of the present invention is to provide a means for dispersing cell aggregates without damaging the cells, such that a sufficient multiplication rate can be obtained in a subculture. According to the present invention, provided is a cell-suspension processing device which disperses cell aggregates included in a cell suspension. The device is provided with: an inlet for taking in the cell suspension; an outlet for discharging the processed cell suspension; and a flow path which is provided between the inlet and the outlet, and which is capable of holding the cell suspension. The flow path has, provided thereto, a liquid delivery pump for causing the cell suspension inside to flow, a cell-dispersion-degree measurement instrument for measuring the dispersion degree of cells in the cell suspension, and a narrow part for imparting shearing force to the cell suspension flowing inside. The cell-suspension processing device is further provided with a control unit for controlling at least the liquid delivery pump on the basis of data obtained by the cell-dispersion-degree measurement instrument. The control unit determines whether the cells have attained a prescribed dispersion degree on the basis of the data obtained by the cell-dispersion-degree measurement instrument, and, in cases when the cells have not attained the prescribed dispersion degree, drives the liquid delivery pump such that the cell suspension is passed through the narrow part.
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