公开(公告)号：US20130274129A1

公开(公告)日：2013-10-17

申请号：US13856978

申请日：2013-04-04

Applicant: GENEART AG ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Federico KATZEN ,  Jonathan Chesnut ,  Frank Notka ,  Matthias Arnold ,  Kevin Hoff ,  Stephen Whiteside

IPC: C07K14/195 ,  C12N15/66 ,  C12N15/10

CPC classification number: C12N15/1082 ,  C07K14/195 ,  C12N9/90 ,  C12N9/96 ,  C12N15/102 ,  C12N15/1086 ,  C12N15/1093 ,  C12N15/66 ,  C12Y599/01002

Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

Abstract translation: 本发明一般涉及用于设计和产生功能性DNA结合效应分子和相关定制服务，工具包和功能测定的组合物和方法。 在一些方面，本发明提供了用于有效组装定制的TAL效应分子的方法和工具。 此外，本发明涉及TAL效应分子的用途和通过例如定制测定法对这种TAL的功能评估。

公开(公告)号：US20160264962A1

公开(公告)日：2016-09-15

申请号：US14811363

申请日：2015-07-28

Applicant: Life Technologies Corporation ,  GeneArt Ag

Inventor： Federico KATZEN ,  Jonathan Chesnut ,  Frank Notka ,  Matthias Arnold ,  Kevin Hoff ,  Stephen Whiteside

IPC: C12N15/10 ,  C12N9/90 ,  C07K14/195 ,  C12N9/96

CPC classification number: C12N15/1082 ,  C07K14/195 ,  C12N9/90 ,  C12N9/96 ,  C12N15/102 ,  C12N15/1086 ,  C12N15/1093 ,  C12N15/66 ,  C12Y599/01002

Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

公开(公告)号：US20140228558A1

公开(公告)日：2014-08-14

申请号：US14034449

申请日：2013-09-23

Applicant: GENEART AG

Inventor： David RAAB ,  Marcus Graf ,  Frank Notka ,  Ralf Wagner

IPC: G06F19/22 ,  C07K14/54 ,  C07K14/535 ,  C07K14/435

CPC classification number: G16B25/00 ,  C07K14/43595 ,  C07K14/535 ,  C07K14/5443 ,  G16B20/00 ,  G16B30/00

Abstract: The invention relates to a method for optimizing a nucleotide sequence for expression of a protein on the basis of the amino acid sequence of the protein, in which for a particular region there is specification of a test sequence with m optimization positions on which the codon occupation is varied, a quality function being used to ascertain the optimal codon occupation on these optimization positions, and one or more codons of this optimal occupation being specified as codons of the optimized nucleotide sequence. These steps are iterated, with the codons of the optimized nucleotide sequence which are specified in the preceding steps remaining unchanged in subsequent iteration steps. The invention additionally relates to a device for carrying out this method.

Abstract translation: 本发明涉及一种用于基于蛋白质的氨基酸序列优化蛋白质表达的核苷酸序列的方法，其中对于特定区域，存在具有m个优化位置的测试序列的指定，其中密码子占据 变化，使用质量函数来确定这些优化位置的最佳密码子占有，并且将该最佳职业的一个或多个密码子指定为优化的核苷酸序列的密码子。 重复这些步骤，其中前面步骤中指定的优化的核苷酸序列的密码子在随后的迭代步骤中保持不变。 本发明还涉及一种用于执行该方法的装置。

公开(公告)号：US10273486B2

公开(公告)日：2019-04-30

申请号：US14483142

申请日：2014-09-10

Applicant: GENEART AG

Inventor： Frank Notka ,  Marcus Graf ,  Doris Baumann ,  Ralf Wagner ,  David Raab

IPC: C12N15/63 ,  C07K14/435

Abstract: The invention relates to nucleic acid modifications for a directed expression modulation by the targeted insertion or removal of CpG dinucleotides. The invention also relates to modified nucleic acids and expression vectors.

公开(公告)号：US20160186166A1

公开(公告)日：2016-06-30

申请号：US14964060

申请日：2015-12-09

Applicant: Life Technologies Corporation ,  Thermo Fisher Scientific Geneart Gmbh ,  Life Technologies As

Inventor： Thomas POEHMERER ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02 ,  B01L3/00 ,  C25B3/10

CPC classification number: C12N15/101 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C5/026 ,  B03C2201/26 ,  C25B3/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US10407676B2

公开(公告)日：2019-09-10

申请号：US14964060

申请日：2015-12-09

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20200010827A1

公开(公告)日：2020-01-09

申请号：US16518099

申请日：2019-07-22

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).