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    Enzyme isolated from a Bifidobacterium
    3.
    发明授权
    Enzyme isolated from a Bifidobacterium 有权

    公开(公告)号：US06555348B2

    公开(公告)日：2003-04-29

    申请号：US09865621

    申请日：2001-05-29

    申请人： Flemming Jørgensen ,  Ole Cai Hansen ,  Peter Stougaard

    发明人： Flemming Jørgensen ,  Ole Cai Hansen ,  Peter Stougaard

    IPC分类号： C12P1944

    CPC分类号： C12Y302/01023 ,  A01K2217/05 ,  C12N9/2471

    摘要： The present invention concerns a new &bgr;-galactosidase with transgalactosylating activity isolated from Bifidobacterium bifidum and a truncated enzyme where the C-terminal end of the &bgr;-galactosidase protein has been deleted, resulting in an enzyme with a higher transgalactosylating activity than hydrolase activity. When lactose is used as a substrate, galacto-oligosaccharides are products of the transgalactosylase activity. Galacto-oligosaccharides enhance growth of health-promoting Bifidobacterium that may be used in a number of applications in the dairy industry.

    Recombinant hexose oxidase, a method of producing same and use of such enzyme
    4.
    发明授权
    Recombinant hexose oxidase, a method of producing same and use of such enzyme 失效
    重组己糖氧化酶，其生产方法和使用这种酶

    公开(公告)号：US08338153B2

    公开(公告)日：2012-12-25

    申请号：US12825510

    申请日：2010-06-29

    申请人： Peter Stougaard ,  Ole Cai Hansen

    发明人： Peter Stougaard ,  Ole Cai Hansen

    IPC分类号： C12N9/04 ,  A61K38/44 ,  A61K47/00 ,  A61K8/00 ,  A21D2/00 ,  A21D8/04 ,  A21D13/00 ,  A23C9/00 ,  A23K1/165 ,  A23L2/00

    CPC分类号： C12P21/02 ,  C12N9/0006

    摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

    摘要翻译： 通过重组DNA技术生产己糖氧化酶的方法，重组己糖氧化酶以及这种酶的使用，特别是在制造面团和乳制品，动物饲料，药物，化妆品，牙科护理产品等食品的制造中 的内酯。 编码酶的DNA的合适来源是海藻类物种，包括软骨including，，鸢尾虱和uthor and。 在有用的实施方案中，重组己糖氧化酶由巴斯德毕赤酵母，酿酒酵母或大肠杆菌产生。

    Recombinant hexose oxidase
    5.
    发明申请
    Recombinant hexose oxidase 有权
    重组己糖氧化酶

    公开(公告)号：US20090275103A1

    公开(公告)日：2009-11-05

    申请号：US12396954

    申请日：2009-03-03

    申请人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

    发明人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

    IPC分类号： C12N9/04

    CPC分类号： C12N9/0006 ,  A01N63/00

    摘要： Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i) (SEQ ID NO: 1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii) (SEQ ID NO: 2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii) (SEQ ID NO: 3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv) (SEQ ID NO: 4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v) (SEQ ID NO: 5) Tyr-Tyr-Phe-Lys (vi) (SEQ ID NO: 6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii) (SEQ ID NO: 7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii) (SEQ ID NO: 8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met where X represents an amino acid selected from the group consisting of Ala, Arg, Asn, Asp, Asx, Cys, Gln, Glu, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr and Val.

    摘要翻译： 使用制剂作为抗微生物剂或作为抗氧化剂; 其中所述制剂包含具有己糖氧化酶活性的多肽，所述多肽为基本上纯的形式，所述多肽包含至少一个选自下组的氨基酸序列：（i）（SEQ ID NO：1）Tyr-Glu- Pro-Tyr-Gly-Gly-Val-Pro（ii）（SEQ ID NO：2）Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly-Tyr-Asp-XXX -Gly-Tyr-X-Val-Ser-Ser-（iii）（SEQ ID NO：3）Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu -X-Phe-（iv）（SEQ ID NO：4）Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His-Thr（v）（SEQ ID NO： 5）Tyr-Tyr-Phe-Lys（vi）（SEQ ID NO：6）Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly-Thr-X-Asp ）（SEQ ID NO：7）Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp（viii）（SEQ ID NO：8）X-Ile-Arg-Asp-Phe-Tyr -Glu-Glu-Met，其中X表示选自Ala，Arg，Asn，Asp，Asx，Cys，Gln，Glu，Glx，Gly，His，Ile，Leu，Lys，Met，Phe， Pro，Ser，Thr，Trp，Tyr和Val。

    Recombinant hexose oxidase
    6.
    发明授权
    Recombinant hexose oxidase 有权
    重组己糖氧化酶

    公开(公告)号：US08178090B2

    公开(公告)日：2012-05-15

    申请号：US12396954

    申请日：2009-03-03

    申请人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

    发明人： Peter Stougaard ,  Ole Cai Hansen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

    IPC分类号： A61K38/44 ,  A61K8/00 ,  A61K8/66 ,  C12N9/04 ,  A23K1/165 ,  A21D8/04 ,  C12N15/53 ,  C12N15/70 ,  C12N15/80

    CPC分类号： C12N9/0006 ,  A01N63/00

    摘要： Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i)(SEQ ID NO: 1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii)(SEQ ID NO: 2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii)(SEQ ID NO: 3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv)(SEQ ID NO: 4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v)(SEQ ID NO: 5) Tyr-Tyr-Phe-Lys (vi)(SEQ ID NO: 6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii)(SEQ ID NO: 7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii)(SEQ ID NO: 8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met where X represents an amino acid selected from the group consisting of Ala, Arg, Asn, Asp, Asx, Cys, Gln, Glu, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr and Val.

    摘要翻译： 使用制剂作为抗微生物剂或作为抗氧化剂; 其中所述制剂包含具有己糖氧化酶活性的多肽，所述多肽为基本上纯的形式，所述多肽包含至少一个选自下组的氨基酸序列：（i）（SEQ ID NO：1）Tyr-Glu- Pro-Tyr-Gly-Gly-Val-Pro（ii）（SEQ ID NO：2）Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly-Tyr-Asp-XXX -Gly-Tyr-X-Val-Ser-Ser-（iii）（SEQ ID NO：3）Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu -X-Phe-（iv）（SEQ ID NO：4）Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His-Thr（v）（SEQ ID NO： 5）Tyr-Tyr-Phe-Lys（vi）（SEQ ID NO：6）Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly-Thr-X-Asp ）（SEQ ID NO：7）Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp（viii）（SEQ ID NO：8）X-Ile-Arg-Asp-Phe-Tyr -Glu-Glu-Met，其中X表示选自Ala，Arg，Asn，Asp，Asx，Cys，Gln，Glu，Glx，Gly，His，Ile，Leu，Lys，Met，Phe， Pro，Ser，Thr， Trp，Tyr和Val。

    Recombinant hexose oxidase, a method of producing same and use of such enzyme
    10.
    发明授权
    Recombinant hexose oxidase, a method of producing same and use of such enzyme 失效
    重组己糖氧化酶，其生产方法和使用这种酶

    公开(公告)号：US06251626B1

    公开(公告)日：2001-06-26

    申请号：US08669304

    申请日：1996-09-11

    申请人： Peter Stougaard ,  Ole Cai Hansen

    发明人： Peter Stougaard ,  Ole Cai Hansen

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  A21D8/04 ,  A23K30/18 ,  A23L3/3571 ,  A23L27/24 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C12P17/02 ,  C12P21/02

    摘要： A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

    摘要翻译： 通过重组DNA技术生产己糖氧化酶的方法，重组己糖氧化酶以及这种酶的使用，特别是在制造面团和乳制品，动物饲料，药物，化妆品，牙科护理产品等食品的制造中 的内酯。 编码酶的DNA的合适来源是海藻类物种，包括软骨including，，鸢尾虱和uthor and。 在有用的实施方案中，重组己糖氧化酶由巴斯德毕赤酵母，酿酒酵母或大肠杆菌产生。