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51 条记录 (耗时 0.041 秒)

    (R)-2,3-butanediol dehydrogenase
    1.
    发明授权
    (R)-2,3-butanediol dehydrogenase 失效
    （R）-2,3-丁二醇脱氢酶

    公开(公告)号：US06818426B2

    公开(公告)日：2004-11-16

    申请号：US10020674

    申请日：2001-10-30

    申请人： Hiroaki Yamamoto ,  Keiko Onodera ,  Yoshiki Tani

    发明人： Hiroaki Yamamoto ,  Keiko Onodera ,  Yoshiki Tani

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  C12P7/18 ,  C12Y101/01004

    摘要： The objective of the present invention is to produce a novel (R)-2,3-butanediol dehydrogenase useful for the production of ketones, alcohols, and particularly optically active vicinal diols. It has been found that Pichia angusta produces novel (R)-2,3-butanediol dehydrogenase that shows the high activity and high stereoselectivity. Further, polynucleotide encoding this (R)-2,3-butanediol dehydrogenase was cloned, and the nucleotide sequence thereof was determined. The expression of the glycerol dehydrogenase was carried out in heterologous microorganisms.

    摘要翻译： 本发明的目的是制备可用于生产酮，醇，特别是光学活性连位二醇的新型（R）-2,3-丁二醇脱氢酶。 已经发现毕赤酵母产生新的（R）-2,3-丁二醇脱氢酶，其显示出高活性和高立体选择性。 此外，克隆编码该（R）-2,3-丁二醇脱氢酶的多核苷酸，测定其核苷酸序列。 甘油脱氢酶的表达在异源微生物中进行。

    32142, 21481, 25964, 21686, novel human dehydrogenase molecules and uses therefor
    3.
    发明授权
    32142, 21481, 25964, 21686, novel human dehydrogenase molecules and uses therefor 有权
    32142,21481,25964,21686，新型人脱氢酶分子及其用途

    公开(公告)号：US06613555B2

    公开(公告)日：2003-09-02

    申请号：US09816760

    申请日：2001-03-23

    申请人： Rachel Meyers ,  William James Cook ,  Mark Williamson ,  Laura A. Rudolph-Owen

    发明人： Rachel Meyers ,  William James Cook ,  Mark Williamson ,  Laura A. Rudolph-Owen

    IPC分类号： C12N904

    CPC分类号： C12N9/0004 ,  C07K2319/00

    摘要： The invention provides isolated nucleic acids molecules, designated DHDR nucleic acid molecules, which encode novel DHDR-related dehydrogenase molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing DHDR nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DHDR gene has been introduced or disrupted. The invention still further provides isolated DHDR proteins, fusion proteins, antigenic peptides and anti-DHDR antibodies. Diagnostic methods utilizing compositions of the invention are also provided.

    摘要翻译： 本发明提供编码新型DHDR相关脱氢酶分子的分离的核酸分子，命名为DHDR核酸分子。 本发明还提供了反义核酸分子，含有DHDR核酸分子的重组表达载体，其中引入了表达载体的宿主细胞，以及DHDR基因导入或破坏的非人类转基因动物。 本发明还进一步提供了分离的DHDR蛋白，融合蛋白，抗原肽和抗DHDR抗体。 还提供了利用本发明组合物的诊断方法。

    Isolated proteins having retinol dehydrogenase activity, and which associate with retinol binding receptors
    5.
    发明授权
    Isolated proteins having retinol dehydrogenase activity, and which associate with retinol binding receptors 失效
    分离的蛋白质具有视黄醇脱氢酶活性，并与视黄醇结合受体相关

    公开(公告)号：US06399344B1

    公开(公告)日：2002-06-04

    申请号：US08937993

    申请日：1997-09-26

    申请人： Ulf Eriksson ,  Andras Simon ,  Anna Romert

    发明人： Ulf Eriksson ,  Andras Simon ,  Anna Romert

    IPC分类号： C12N904

    CPC分类号： C12N9/0006

    摘要： Isolated membrane associated proteins which have a molecular weight of from about 30 kD to about 36 kilodaltons as determined by SDS-PAGE, are disclosed. The proteins have cis retinol dehydrogenase activity, such as 9, 11 or 13 cis retinol dehydrogenase activity, or cis activity at more than one position. Other trans-retinol dehydrogenases are also disclosed. Nucleic acid molecules which code for the proteins are also disclosed.

    摘要翻译： 公开了通过SDS-PAGE测定的具有约30kD至约36千道尔顿的分离膜相关蛋白。 蛋白质具有顺式视黄醇脱氢酶活性，例如9,11或13顺式视黄醇脱氢酶活性，或多于一个位置的顺式活性。 还公开了其他反式视黄醇脱氢酶。 还公开了编码蛋白质的核酸分子。

    3-Hydroxy-3-methylglutaryl-CoA reductase polynucleotides in isoprenoid production
    6.
    发明授权
    3-Hydroxy-3-methylglutaryl-CoA reductase polynucleotides in isoprenoid production 有权
    3-羟基-3-甲基戊二酰辅酶A还原酶多核苷酸在类异戊二烯生产中的应用

    公开(公告)号：US06284506B1

    公开(公告)日：2001-09-04

    申请号：US09306595

    申请日：1999-05-06

    申请人： Tatsuo Hoshino ,  Kazuyuki Ojima ,  Yutaka Setoguchi

    发明人： Tatsuo Hoshino ,  Kazuyuki Ojima ,  Yutaka Setoguchi

    IPC分类号： C12N904

    CPC分类号： C12N9/0004 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/88 ,  C12P23/00

    摘要： The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate (i.e., HMG-CoA synthase, HMG-CoA reductase, mevalonate kinase, mevalonate pyrophosphate decarboxylase, and FPP synthase). Vectors and plasmids including such DNA, are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.

    摘要翻译： 本发明涉及编码甲羟戊酸途径中的酶或从异戊烯焦磷酸至法呢基焦磷酸（即HMG-CoA合成酶，HMG-CoA还原酶，甲羟戊酸激酶，甲羟戊酸焦磷酸脱羧酶和FPP合成酶）的途径的分离DNA序列。 载体和包括这种DNA的质粒也被列出。 本发明还包括由这些DNA转化的宿主细胞，或含有这些DNA的载体或质粒。 还提供了使用这种转化的宿主细胞产生类异戊二烯和类胡萝卜素的方法。

    Mouse and human 9-cis-retinol dehydrogenase
    7.
    发明授权
    Mouse and human 9-cis-retinol dehydrogenase 失效
    小鼠和人类9-顺式 - 视黄醇脱氢酶

    公开(公告)号：US06171837B2

    公开(公告)日：2001-01-09

    申请号：US08940424

    申请日：1997-09-29

    申请人： William S. Blaner ,  Roseann Piantedosi Zott ,  Mary V. Gamble ,  James R. Mertz

    发明人： William S. Blaner ,  Roseann Piantedosi Zott ,  Mary V. Gamble ,  James R. Mertz

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  A61K48/00

    摘要： This invention provides an isolated nucleic acid molecule (SEQ ID NO:1) encoding a human 9-cis-retinol dehydrogenase. Also provided is a 9-cis-retinol dehydrogenase encoded by the isolated nucleic acid molecule, wherein the 9-cis-retinol dehydrogenase comprises the amino acid sequence of SEQ. ID NO: 2. This invention also provides isolated nucleic acid molecules comprising the nucleotide sequence shown in any of SEQ. ID NOS: 6, 7, and 8 encoding mouse 9-cis-retinol dehydrogenases.

    摘要翻译： 本发明提供了编码人9-顺 - 视黄醇脱氢酶的分离的核酸分子（SEQ ID NO：1）。 还提供了由分离的核酸分子编码的9-顺式 - 视黄醇脱氢酶，其中9-顺式 - 视黄醇脱氢酶包含SEQ ID NO：1的氨基酸序列。 本发明还提供了分离的核酸分子，其包含SEQ ID NO：2中任一个所示的核苷酸序列。 ID NOS：6,7和8编码小鼠9-顺 - 视黄醇脱氢酶。

    Isolated nucleic acids encoding methylenetetrahydrofolate dehydrogenase enzymes
    10.
    发明授权
    Isolated nucleic acids encoding methylenetetrahydrofolate dehydrogenase enzymes 失效
    分离的编码亚甲基四氢叶酸脱氢酶的核酸

    公开(公告)号：US06680428B2

    公开(公告)日：2004-01-20

    申请号：US09903814

    申请日：2001-07-12

    申请人： Saverio Carl Falco ,  Layo O. Famodu ,  J. Antoni Rafalski

    发明人： Saverio Carl Falco ,  Layo O. Famodu ,  J. Antoni Rafalski

    IPC分类号： C12N904

    CPC分类号： C12N9/0044

    摘要： This invention relates to an isolated nucleic acid fragment encoding a tetrahydrofolate metabolism enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tetrahydrofolate metabolism enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tetrahydrofolate metabolism enzyme in a transformed host cell.

    摘要翻译： 本发明涉及编码四氢叶酸代谢酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分四氢叶酸代谢酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中四氢叶酸代谢酶水平的变化 。