公开(公告)号：US07202069B2

公开(公告)日：2007-04-10

申请号：US10766421

申请日：2004-01-27

申请人： Masatake Kudoh ,  Hiroaki Yamamoto

发明人： Masatake Kudoh ,  Hiroaki Yamamoto

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12P7/02 ,  C12P21/04 ,  C12Q1/00 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P7/22

摘要： This invention provides (R)-2-octanol dehydrogenase that catalyzes oxidation-reduction reaction using NAD+ (NADH) as a coenzyme and the genes that encodes them. The enzymes of this invention can be obtained from microorganisms such as the genera Pichia, Candida, and Ogataea, and so on. It is possible to produce alcohols, in particular, alcohols such as (S)-4-halo-3-hydroxybutyric acid esters and (R)-propoxybenzene derivatives by reducing ketones with this (R)-2-octanol dehydrogenase. Moreover, the (R)-2-octanol dehydrogenase of this invention is excellent in activity and stereoselectivity.

摘要翻译： 本发明提供使用NADH（NADH）作为辅酶催化氧化还原反应的（R）-2-辛醇脱氢酶和编码它们的基因。 本发明的酶可以从毕赤酵母属，念珠菌属，Ogataea等微生物获得。 通过用（R）-2-辛醇脱氢酶还原酮，可以生产醇，特别是醇，如（S）-4-卤代-3-羟基丁酸酯和（R） - 丙氧基苯衍生物。 此外，本发明的（R）-2-辛醇脱氢酶的活性和立体选择性优异。

公开(公告)号：US06706507B2

公开(公告)日：2004-03-16

申请号：US09978758

申请日：2001-10-16

申请人： Masatake Kudoh ,  Hiroaki Yamamoto

发明人： Masatake Kudoh ,  Hiroaki Yamamoto

IPC分类号： C12N904

CPC分类号： C12N9/0006 ,  C12P7/22

摘要： This invention provides (R)-2-octanol dehydrogenase that catalyzes oxidation-reduction reaction using NAD+ (NADH) as a coenzyme and the genes that encodes them. The enzymes of this invention can be obtained from microorganisms such as the genera Pichia, Candida, and Ogataea, and so on. It is possible to produce alcohols, in particular, alcohols such as (S)-4-halo-3-hydroxybutyric acid esters and (R)-propoxybenzene derivatives by reducing ketones with this (R)-2-octanol dehydrogenase. Moreover, the (R)-2-octanol dehydrogenase of this invention is excellent in activity and stereoselectivity.

公开(公告)号：US20050003500A1

公开(公告)日：2005-01-06

申请号：US10858707

申请日：2004-06-02

申请人： Masatake Kudoh ,  Hiroaki Yamamoto

发明人： Masatake Kudoh ,  Hiroaki Yamamoto

IPC分类号： C12N15/09 ,  C07B53/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12P7/16 ,  C12P7/18 ,  C12P41/00 ,  C12R1/01

CPC分类号： C12P7/16 ,  C12P7/18 ,  Y02E50/10

摘要： An objective of the present invention is to produce a novel (2S,3S)-2,3-butanediol dehydrogenase useful for the production of ketones, alcohols, and particularly optically active vicinal diols. Zoogloea ramigera has been found to produce a novel (2S,3S)-2,3-butanediol dehydrogenase having high activity and high stereoselectivity. Furthermore, a DNA chain encoding this (2S,3S)-2,3-butanediol dehydrogenase was cloned and the nucleotide sequence thereof was determined. The expression of the (2S,3S)-2,3-butanediol dehydrogenase was carried out in a heterologous microorganism.

摘要翻译： 本发明的目的是制备可用于生产酮，醇，特别是光学活性连位二醇的新型（2S，3S）-2,3-丁二醇脱氢酶。 已经发现Zoogloea ramigera生产出具有高活性和高立体选择性的新型（2S，3S）-2,3-丁二醇脱氢酶。 此外，克隆编码该（2S，3S）-2,3-丁二醇脱氢酶的DNA链，并测定其核苷酸序列。 （2S，3S）-2,3-丁二醇脱氢酶的表达在异源微生物中进行。

公开(公告)号：US10413670B2

公开(公告)日：2019-09-17

申请号：US14344581

申请日：2012-09-12

申请人： Masatake Kudoh

发明人： Masatake Kudoh

IPC分类号： A61M5/30 ,  A61M5/20 ,  A61M5/31

摘要： A needleless syringe comprises an enclosing unit for an injection objective substance, a pressurizing unit for the injection objective substance, a discharge unit having a flow passage for allowing the injection objective substance to flow therethrough so that the injection objective substance is discharged from an open end of the flow passage to an injection target area, the open end being formed so that an area thereof is smaller than a flow passage area of the enclosing unit, and a minute pore unit including a minute pore having a flow passage area smaller than the area of the open end of the discharge unit and which is arranged on an outer side of the open end so that the injection objective substance discharged from the open end passes through the minute pore and arrives at a side of the injection target area.

公开(公告)号：US20150032082A1

公开(公告)日：2015-01-29

申请号：US14344581

申请日：2012-09-12

申请人： Masatake Kudoh

发明人： Masatake Kudoh

IPC分类号： A61M5/30

CPC分类号： A61M5/30 ,  A61M5/2046 ,  A61M5/3007 ,  A61M2005/3132

摘要： A needleless syringe comprises an enclosing unit for an injection objective substance, a pressurizing unit for the injection objective substance, a discharge unit having a flow passage for allowing the injection objective substance to flow therethrough so that the injection objective substance is discharged from an open end of the flow passage to an injection target area, the open end being formed so that an area thereof is smaller than a flow passage area of the enclosing unit, and a minute pore unit including a minute pore having a flow passage area smaller than the area of the open end of the discharge unit and which is arranged on an outer side of the open end so that the injection objective substance discharged from the open end passes through the minute pore and arrives at a side of the injection target area.

摘要翻译： 无针注射器包括用于注射目标物质的封闭单元，用于注射物镜物质的加压单元，具有用于允许注射物质流过其中的流动通道的排出单元，使得注射物质从开口端排出 所述开口端形成为使得其面积小于所述封闭单元的流路面积，并且所述微孔单元包括具有小于所述区域的流路面积的微孔 并且其布置在开口端的外侧，使得从开口端排出的注入物质物质通过微孔并到达注射目标区域的一侧。