公开(公告)号：US08586325B2

公开(公告)日：2013-11-19

申请号：US13891406

申请日：2013-05-10

Applicant: Fei Mao ,  Hui Cen

Inventor： Fei Mao ,  Hui Cen

IPC: C12Q1/37 ,  A61K38/14 ,  C07D279/08

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。

公开(公告)号：US08092784B2

公开(公告)日：2012-01-10

申请号：US12095322

申请日：2006-11-21

Applicant: Fei Mao ,  Hui Cen ,  Wai-yee Leung

Inventor： Fei Mao ,  Hui Cen ,  Wai-yee Leung

IPC: A61B10/00 ,  C12Q1/68

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。

公开(公告)号：US08778627B2

公开(公告)日：2014-07-15

申请号：US13326009

申请日：2011-12-14

Applicant: Fei Mao ,  Hui Cen ,  Wai-Yee Leung

Inventor： Fei Mao ,  Hui Cen ,  Wai-Yee Leung

IPC: C12Q1/40 ,  C12Q1/48 ,  C12Q1/37 ,  C07D263/60

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或诊断疾病。

公开(公告)号：US20130236888A1

公开(公告)日：2013-09-12

申请号：US13891406

申请日：2013-05-10

Applicant: Fei Mao ,  Hui Cen

Inventor： Fei Mao ,  Hui Cen

IPC: G01N21/64 ,  C07K7/06 ,  C07D501/00 ,  C07D417/06

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

公开(公告)号：US20090148386A1

公开(公告)日：2009-06-11

申请号：US12095322

申请日：2006-11-21

Applicant: Fei Mao ,  Hui Cen ,  Wai-yee Leung

Inventor： Fei Mao ,  Hui Cen ,  Wai-yee Leung

IPC: A61B10/00 ,  C12Q1/68 ,  G01N33/573

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。

公开(公告)号：US08790892B2

公开(公告)日：2014-07-29

申请号：US13439821

申请日：2012-04-04

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

IPC: G01N1/30

CPC classification number: C09B1/516 ,  A61K49/0052 ,  C09B1/501 ,  C09B1/51 ,  C09B1/5155 ,  G01N1/30 ,  G01N21/6428

Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.

Abstract translation: 本发明公开了可用作细胞污渍的新的取代的蒽醌染料。 在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。 本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

公开(公告)号：US08759055B2

公开(公告)日：2014-06-24

申请号：US12020459

申请日：2008-01-25

Applicant: Adam Heller ,  Nicholas Mano ,  Hyug-Han Kim ,  Yongchao Zhang ,  Fei Mao ,  Ting Chen ,  Scott C. Barton

Inventor： Adam Heller ,  Nicholas Mano ,  Hyug-Han Kim ,  Yongchao Zhang ,  Fei Mao ,  Ting Chen ,  Scott C. Barton

IPC: C12N11/18 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/72 ,  H01M8/16

CPC classification number: H01M8/16 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/723 ,  Y02E60/527

Abstract: A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a lacasse, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.

Abstract translation: 燃料电池设有阳极和阴极。 阳极与阳极酶电连通，阴极与阴极酶电连通。 阳极酶优选为氧化酶或脱氢酶。 阴极酶是含铜酶，例如lacasse，抗坏血酸氧化酶，血清白蛋白或胆红素氧化酶。 优选地，阴极酶在生理条件下是可操作的。 氧化还原聚合物用于将阳极酶连接到阳极，阴极酶连接到阴极。 燃料电池的尺寸可以非常小，因为它不需要膜，密封件或外壳。 燃料电池可以与诸如人类的生物系统结合使用，因为其可以在生理条件下操作。 由于其在生理条件下的尺寸和可操作性，燃料电池对于要求植入人体的电源（例如各种医疗应用）的应用是特别有意义的。

公开(公告)号：US08728297B2

公开(公告)日：2014-05-20

申请号：US11279694

申请日：2006-04-13

Applicant: Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

Inventor： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC: G01N27/327

CPC classification number: G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

Abstract: A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

Abstract translation: 描述了利用不可浸出或可扩散的氧化还原介体的传感器。 传感器包括用于保持样品与工作电极电解接触的样品室，并且在至少一些情况下，传感器还含有不可浸出或可扩散的第二电子转移剂。 传感器和/或所使用的方法响应于能够与介体引起的背景信号区分的分析物产生传感器信号。 本发明可用于使用诸如库仑法，电流分析法和电位法的技术来确定生物流体如血液或血清中的生物分子如葡萄糖或乳酸盐的浓度。 通常可以提供能够催化生物分子的电氧化或电还原的酶作为第二电子转移剂。

公开(公告)号：US08530195B2

公开(公告)日：2013-09-10

申请号：US12820983

申请日：2010-06-22

Applicant: Fei Mao ,  Wai-Yee Leung ,  Xing Xin

Inventor： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

Abstract translation: 提供二聚和三聚体核酸染料以及相关系统和方法。 这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。 在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。 本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

公开(公告)号：US08512534B2

公开(公告)日：2013-08-20

申请号：US13215009

申请日：2011-08-22

Applicant: Fei Mao ,  Adam Heller

Inventor： Fei Mao ,  Adam Heller

IPC: C07F15/00 ,  G01N27/327

CPC classification number: A61B5/1486 ,  A61B5/14532 ,  A61B5/1477 ,  C07F9/005 ,  C07F15/0026 ,  C07F15/0053 ,  C07F15/025 ,  C07F15/065 ,  C08F8/42 ,  C08F8/44 ,  C09B57/10 ,  C12Q1/004

Abstract: Novel transition metal complexes of iron, cobalt, ruthenium, osmium, and vanadium are described. The transition metal complexes can be used as redox mediators in enzyme based electrochemical sensors. In such instances, transition metal complexes accept electrons from, or transfer electrons to, enzymes at a high rate and also exchange electrons rapidly with the sensor. The transition metal complexes include at least one substituted or unsubstituted biimidazole ligand and may further include a second substituted or unsubstituted biimidazole ligand or a substituted or unsubstituted bipyridine or pyridylimidazole ligand. Transition metal complexes attached to polymeric backbones are also described.

Abstract translation: 描述了铁，钴，钌，锇和钒的新型过渡金属络合物。 过渡金属络合物可用作酶基电化学传感器中的氧化还原介质。 在这种情况下，过渡金属配合物以高速率接受电子或将电子转移到酶，并且还与传感器快速交换电子。 过渡金属络合物包括至少一个取代或未取代的双咪唑配体，并且还可以包括第二取代或未取代的双咪唑配体或取代或未取代的联吡啶或吡啶基咪唑配体。 还描述了连接到聚合物主链上的过渡金属络合物。