公开(公告)号：US08092784B2

公开(公告)日：2012-01-10

申请号：US12095322

申请日：2006-11-21

Applicant: Fei Mao ,  Hui Cen ,  Wai-yee Leung

Inventor： Fei Mao ,  Hui Cen ,  Wai-yee Leung

IPC: A61B10/00 ,  C12Q1/68

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。

公开(公告)号：US20090148386A1

公开(公告)日：2009-06-11

申请号：US12095322

申请日：2006-11-21

Applicant: Fei Mao ,  Hui Cen ,  Wai-yee Leung

Inventor： Fei Mao ,  Hui Cen ,  Wai-yee Leung

IPC: A61B10/00 ,  C12Q1/68 ,  G01N33/573

CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

Abstract translation: 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。