公开(公告)号：US06773920B1

公开(公告)日：2004-08-10

申请号：US09937837

申请日：2002-01-07

Applicant: Brian Dalby ,  Robert P. Bennett

Inventor： Brian Dalby ,  Robert P. Bennett

IPC: C12N1587

CPC classification number: C07K14/005 ,  A61K48/0025 ,  C07K2319/00 ,  C12N15/85 ,  C12N15/87 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2840/20

Abstract: The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that contains the target gene) attached to a translocating polypeptide. In another embodiment, expression of a target gene product in a cell in culture that contains a target gene under control of one or more regulatory elements is modulated by contacting the cell in culture with one or more regulatory agents attached to a translocating polypeptide. The one or more regulatory agents are translocated into the cell in culture and interact therein with the one or more regulatory elements to modulate expression of the target gene product by the cell.

Abstract translation: 本发明提供了通过使培养物中的细胞与连接于易位多肽的细胞过程修饰分子接触来调节细胞过程的方法。 例如，在一个实施方案中，通过使培养中的细胞与附着于易位多肽的多核苷酸（含有靶基因）接触，用靶基因转染培养细胞。 在另一个实施方案中，通过使培养物中的细胞与连接于易位多肽的一种或多种调节剂接触来调节目标基因产物在含有在一种或多种调节元件控制下的靶基因的培养物中的细胞中的表达。 一种或多种调节剂在培养物中被转运到细胞中并与其中的一种或多种调节元件相互作用以调节细胞对靶基因产物的表达。

公开(公告)号：US20110283019A1

公开(公告)日：2011-11-17

申请号：US12779410

申请日：2010-05-13

Applicant: Robert P. Bennett ,  Vincent R. Re

Inventor： Robert P. Bennett ,  Vincent R. Re

IPC: G06F15/16

CPC classification number: G06F9/541

Abstract: According to one embodiment, a data sources interface of a mainframe receives a transaction request from a user terminal communicating through a network. The transaction request is formatted in a web-based format. A data sources function is called to instruct a mainframe application to perform the requested transaction, and the instruction to the mainframe is formatted in the native format of the mainframe. A transaction result formatted in the native format of the mainframe is received from the mainframe application. The transaction result is modified according to the web-based format to yield a data source, and the data source is sent to the user terminal.

Abstract translation: 根据一个实施例，主机的数据源接口从通过网络通信的用户终端接收事务请求。 交易请求以基于Web的格式进行格式化。 调用数据源功能来指示大型机应用程序执行所请求的事务，并向主机指令格式化为主机的本机格式。 从主机应用程序接收以大型机的本机格式格式化的事务结果。 交易结果根据基于Web的格式进行修改，产生数据源，数据源发送给用户终端。

公开(公告)号：US20090328244A1

公开(公告)日：2009-12-31

申请号：US12409326

申请日：2009-03-23

Applicant: Jonathan D. Chesnut ,  Stewart Shuman ,  Knut R. Madden ,  John A. Heyman ,  Robert P. Bennett

Inventor： Jonathan D. Chesnut ,  Stewart Shuman ,  Knut R. Madden ,  John A. Heyman ,  Robert P. Bennett

IPC: A01K67/027 ,  C12P19/34 ,  C12N5/06 ,  C12N15/11

CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/64

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract translation: 本发明提供用于共价连接核酸分子的组合物，方法和试剂盒。 所述方法包括线入侵步骤，组合物和试剂盒对于进行这些方法是有用的。 例如，共价连接双链（ds）核酸分子的方法可以包括使第一个ds核酸分子接触，该第一个ds核酸分子具有与一个末端的3'末端连接的拓扑异构酶并且具有相同的单链（5'）突出端 末端，具有平末端的第二ds核酸分子，使得5'突出端可以与第二核酸分子的平端的互补序列杂交，并且拓扑异构酶可以共价连接ds核酸分子。 该方法比先前用于共价连接核酸序列的方法更简单和更有效，并且组合物和试剂盒有助于实施该方法，包括定向连接两个或多个ds核酸分子的方法。

公开(公告)号：US4880954A

公开(公告)日：1989-11-14

申请号：US201903

申请日：1988-06-03

Applicant: Robert P. Bennett ,  Kunimitsu Ozaki

Inventor： Robert P. Bennett ,  Kunimitsu Ozaki

IPC: F25D23/12 ,  F25D31/00 ,  H05B6/66

CPC classification number: H05B6/66 ,  F25D23/12 ,  H05B6/808 ,  F25D31/005 ,  Y10T307/398 ,  Y10T307/484

Abstract: A microwave oven and a refrigerator are combined in a single cabinet and share line power supplied to the cabinet. Door interlocks and a manually settable time are provided for disconnecting electrical supply to the compressor of the refrigerator during operation of the magnetron of the microwave oven. The circuitry provided ensures that electrical supply is reconnected to the compressor upon expiration of the timer setting, even if the door of the oven is incidentally left open after use.

Abstract translation: 微波炉和冰箱组合在一个机柜中，并共享供给机柜的线路电源。 提供门互锁和手动设置时间，用于在微波炉的磁控管操作期间断开对冰箱的压缩机的电力供应。 所提供的电路确保了在定时器设置期满时电源重新连接到压缩机，即使烤箱的门在使用后偶然地打开。

公开(公告)号：US4599249A

公开(公告)日：1986-07-08

申请号：US681109

申请日：1984-12-12

Applicant: Robert P. Bennett ,  Robert L. Sokolove

Inventor： Robert P. Bennett ,  Robert L. Sokolove

IPC: B01J2/30 ,  C10L1/32 ,  C10L9/10 ,  F17D1/17 ,  B05D7/00 ,  C08L1/08 ,  C10L9/00 ,  C10L10/00

CPC classification number: F17D1/17 ,  B01J2/30 ,  C10L1/326 ,  C10L9/10 ,  Y10T137/0391

Abstract: An aqueous solution is applied to water insoluble solid particles when flowing from one location to another location to improve the flow characteristics of the particles while inhibiting caking characteristics of the particles. The aqueous solution comprises a water soluble polymer derived from cellulose.

Abstract translation: 当从一个位置流动到另一个位置时，将水溶液施加到水不溶性固体颗粒上，以改善颗粒的流动特性，同时抑制颗粒的结块特性。 水溶液包含衍生自纤维素的水溶性聚合物。

公开(公告)号：US20090326208A1

公开(公告)日：2009-12-31

申请号：US12112649

申请日：2008-04-30

Applicant: John Carrino ,  James Fan ,  Robert P. Bennett ,  Jonathan D. Chesnut ,  Martin A. Gleeson ,  Knut R. Madden

Inventor： John Carrino ,  James Fan ,  Robert P. Bennett ,  Jonathan D. Chesnut ,  Martin A. Gleeson ,  Knut R. Madden

IPC: C07H21/04 ,  C07H1/00

CPC classification number: C12N15/10 ,  C12N9/90 ,  C12N15/111 ,  C12N15/64 ,  C12N15/66 ,  C12N2310/14 ,  C12N2330/30 ,  C12P19/34 ,  C12Q1/6855 ,  C12Y599/01002 ,  C12Q2521/519

Abstract: A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

Abstract translation: 通过使两个或多个ds核苷酸序列与拓扑异构酶接触的条件使得在第一个ds核苷酸序列的至少一个末端的两个末端共价连接的情况下，产生双链（ds）重组核酸分子共价连接的双链（ds）重组核酸分子的方法 提供了拓扑异构酶至第二个ds核苷酸序列的至少一个末端的两个末端。 还提供了一种用于产生共价连接在一条链中的ds重组核酸分子的方法，其通过使两个或更多个ds核苷酸序列与IA型拓扑异构酶接触，使得一个或两个末端的一条链但不是两条链 第一个ds核苷酸序列由拓扑异构酶共价连接。 还提供了用于进行这些方法的组合物，以及由这些方法产生的组合物，以及包含用于方便地实施该方法的组分的试剂盒。

公开(公告)号：US5819672A

公开(公告)日：1998-10-13

申请号：US418074

申请日：1995-04-06

Applicant: Jerrold E. Radway ,  Robert P. Bennett

Inventor： Jerrold E. Radway ,  Robert P. Bennett

IPC: C10B29/06 ,  C23C30/00 ,  F27D1/16 ,  F27D25/00 ,  F27D99/00 ,  F28F13/18 ,  F23J3/00

CPC classification number: F27D1/16 ,  C10B29/06 ,  C23C30/00 ,  F27D99/00 ,  F28F13/187 ,  F27D1/1684 ,  F27D25/00 ,  F28F2245/06 ,  Y10S118/05 ,  Y10S165/904

Abstract: The present invention provides a method and composition for on-line treatment of furnace walls that are subject to deposition of highly reflective ash. The method involves exposing the walls to a darkening agent, or a combination of a darkening agent and a fluxing agent. The darkening agent, or the combined darkening agent and fluxing agent, may be mixed with the coal before it enters the boiler. Preferably, the appropriate walls of the boiler are treated directly with the darkening agent. A preferred embodiment involves direct application of the darkening agent to the water wall.

Abstract translation: 本发明提供了一种用于炉壁的在线处理的方法和组合物，所述方法和组合物经受沉积高反射灰分。 该方法包括将壁暴露于变暗剂或变暗剂与助熔剂的组合。 变黑剂或组合的变暗剂和助熔剂可在煤进入锅炉之前与煤混合。 优选地，锅炉的适当壁直接用变色剂处理。 优选的实施方案涉及将变暗剂直接施用于水壁。

公开(公告)号：US4847722A

公开(公告)日：1989-07-11

申请号：US97680

申请日：1987-09-17

Applicant: Robert P. Bennett

Inventor： Robert P. Bennett

IPC: F24C7/02 ,  F25D23/12 ,  H05B6/66

CPC classification number: H05B6/808 ,  F25D23/12 ,  H05B6/66 ,  Y10T307/438 ,  Y10T307/469

Abstract: A microwave oven and a refrigerator are combined into a single cabinet housing a shared power supply with an electrical supply disconnect for the magnetron of the oven during a start-up power demand by the compressor of the refrigerator and for a period of time depending upon a rating of the compressor.

Abstract translation: 微波炉和冰箱组合成单个机柜，其容纳共享电源，在由冰箱的压缩机启动电力需求期间，具有用于烤箱磁控管的电源断开器，并且一段时间取决于 压缩机的额定值。

公开(公告)号：US4738690A

公开(公告)日：1988-04-19

申请号：US917260

申请日：1986-10-08

Applicant: Jerrold E. Radway ,  Robert P. Bennett

Inventor： Jerrold E. Radway ,  Robert P. Bennett

IPC: B01D51/00 ,  B03C3/013 ,  B03C3/01 ,  B01D53/32

CPC classification number: B03C3/013 ,  B01D51/00

Abstract: A composition of matter is used to treat particles entrained in flue gas. The composition comprises a caustic liquid to adjust the resistivity of the entrained particles. An inert particulate matter supports the caustic liquid to inhibit the chemical action of the caustic liquid until used to adjust the resistivity of the entrained particles. The entrained particles are removed from the flue gas by choosing a caustic liquid to adjust the resistivity of the entrained particles. The caustic liquid is supported on inert particulate matter to inhibit the chemical action of the caustic liquid. The supported caustic liquid is supplied to untreated flue gas at a temperature sufficient to separate the caustic liquid from the inert particulate matter. The separated caustic liquid is mixed with the entrained particles so as to coat the entrained particles. The coated particles are then passed through an electrostatic precipitator to remove them from the flue gas.

Abstract translation: 使用物质的组成来处理夹带在烟道气中的颗粒。 组合物包含用于调节夹带颗粒的电阻率的苛性液体。 惰性颗粒物质支持苛性液体以抑制苛性液体的化学作用，直到用于调节夹带颗粒的电阻率。 通过选择苛性液体来调节夹带颗粒的电阻率，从烟道气中除去夹带的颗粒。 苛性液体负载在惰性颗粒物上，以抑制苛性液体的化学作用。 将负载的苛性液体以足以将苛性液体与惰性颗粒物质分离的温度供应到未处理的烟道气。 将分离的苛性液体与夹带的颗粒混合以涂覆夹带的颗粒。 然后将涂覆的颗粒通过静电除尘器以将其从烟道气中除去。

公开(公告)号：US06916632B2

公开(公告)日：2005-07-12

申请号：US09935280

申请日：2001-08-21

Applicant: Jonathan D. Chesnut ,  Stewart Shuman ,  Knut R. Madden ,  John A. Heyman ,  Robert P. Bennett

Inventor： Jonathan D. Chesnut ,  Stewart Shuman ,  Knut R. Madden ,  John A. Heyman ,  Robert P. Bennett

IPC: A01K67/027 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C40B40/02 ,  C40B50/06 ,  C12P19/34 ,  C12N9/90 ,  C12N15/00 ,  C12P21/03

CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/64

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract translation: 本发明提供用于共价连接核酸分子的组合物，方法和试剂盒。 所述方法包括线入侵步骤，组合物和试剂盒对于进行这些方法是有用的。 例如，共价连接双链（ds）核酸分子的方法可以包括使第一个ds核酸分子接触，该第一个ds核酸分子具有与一个末端的3'末端连接的拓扑异构酶并且具有相同的单链（5'）突出端 末端，具有平末端的第二ds核酸分子，使得5'突出端可以与第二核酸分子的平端的互补序列杂交，并且拓扑异构酶可以共价连接ds核酸分子。 该方法比先前用于共价连接核酸序列的方法更简单和更有效，并且组合物和试剂盒有助于实施该方法，包括定向连接两个或多个ds核酸分子的方法。