公开(公告)号：US20180059118A1

公开(公告)日：2018-03-01

申请号：US15712972

申请日：2017-09-22

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Kristin Weidemaier ,  Christian Sandmann ,  William Shannon Dillmore ,  James L. Schram ,  W. William Stewart ,  Robert E. Pearson ,  Helen Hsieh ,  Steven Keith ,  Rajendra R. Bhat ,  Andrea Liebmann-Vinson ,  Adam Craig Curry ,  Alexander G. Lastovich

IPC: G01N33/58 ,  G01N21/65 ,  G01N33/543

CPC classification number: G01N33/587 ,  G01N21/658 ,  G01N33/54333

Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.

公开(公告)号：US11016095B2

公开(公告)日：2021-05-25

申请号：US15712972

申请日：2017-09-22

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Kristin Weidemaier ,  Christian Sandmann ,  William Shannon Dillmore ,  James L. Schram ,  W. William Stewart ,  Robert E. Pearson ,  Helen Hsieh ,  Steven Keith ,  Rajendra R. Bhat ,  Andrea Liebmann-Vinson ,  Adam Craig Curry ,  Alexander G. Lastovich

IPC: G01N33/58 ,  G01N21/65 ,  G01N33/543

Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.

公开(公告)号：US09874555B2

公开(公告)日：2018-01-23

申请号：US14391340

申请日：2013-03-15

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Kristin Weidemaier ,  Robert L. Campbell ,  Erin Gooch Carruthers ,  Adam C. Curry ,  Kevin G. Dolan ,  Andrea Liebmann-Vinson ,  Wendy Dale Woodley ,  Melody M. H. Kuroda ,  Eric A. Fallows ,  Miroslaw Bartkowiak ,  Scott N. Danhof ,  Gregory S. Kramer ,  Thomas D. Haubert ,  Michael L. Marshall ,  James A. Prescott ,  Randy J. Somerville ,  M. Scott Ulrich ,  David S. Sebba

IPC: C12M1/34 ,  G01N33/543 ,  G01N33/569 ,  C12M1/00 ,  C12M1/26 ,  C12M1/24 ,  G01N35/00 ,  B03C1/01 ,  B03C1/28 ,  B01L3/02 ,  B01L3/00

CPC classification number: G01N33/54333 ,  B01L3/0217 ,  B01L3/502 ,  B01L3/50825 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/10 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12M23/08 ,  C12M23/22 ,  C12M23/38 ,  C12M33/04 ,  C12M41/14 ,  C12M41/36 ,  G01N33/56911 ,  G01N35/0098

Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

公开(公告)号：US20180136204A1

公开(公告)日：2018-05-17

申请号：US15822879

申请日：2017-11-27

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Kristin Weidemaier ,  Robert L. Campbell ,  Erin Gooch Carruthers ,  Adam Craig Curry ,  Kevin G. Dolan ,  Andrea Liebmann-Vinson ,  Wendy Dale Woodley ,  Melody M.H. Kuroda ,  Ammon David Lentz ,  Dwight Livingston ,  Michael Justin Lizzi ,  Artis R. Lockhart ,  Ernie Ritchey ,  Eric A. Fallows ,  Donald E. Gorelick ,  Jack Kessler ,  Spencer Lovette ,  Jeffrey S. Ojala ,  Mark A. Talmer ,  Miroslaw Bartkowiak ,  Scott N. Danhof ,  Gregory S. Kramer ,  Thomas D. Haubert ,  Michael L. Marshall ,  James A. Prescott ,  Randy J. Somerville ,  M. Scott Ulrich ,  David S. Sebba

IPC: G01N33/543 ,  B01L3/02 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  C12M1/24 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34 ,  G01N33/569 ,  G01N35/00

CPC classification number: G01N33/54333 ,  B01L3/0217 ,  B01L3/502 ,  B01L3/50825 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/10 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12M23/08 ,  C12M23/22 ,  C12M23/38 ,  C12M33/04 ,  C12M41/14 ,  C12M41/36 ,  G01N33/56911 ,  G01N35/0098

Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

公开(公告)号：US20150118688A1

公开(公告)日：2015-04-30

申请号：US14391340

申请日：2013-03-15

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Kristin Weidemaier ,  Robert L. Campbell ,  Erin Gooch Carruthers ,  Adam C. Curry ,  Kevin G. Dolan ,  Andrea Liebmann-Vinson ,  Wendy Dale Woodley ,  Melody M.H. Kuroda ,  Ammon David Lentz ,  Dwight Livingston ,  Michael Justin Lizzi ,  Artis R. Lockhart ,  Ernie Ritchey ,  Eric A. Fallows ,  Donald E. Gorelick ,  Jack Kessler ,  Spencer Lovette ,  Jeffrey S. Ojala ,  Mark A. Talmer ,  Miroslaw Bartkowiak ,  Scott N. Danhof ,  Gregory S. Kramer ,  Thomas D. Haubert ,  Michael L. Marshall ,  James A. Prescott ,  Randy J. Somerville ,  M. Scott Ulrich ,  David S. Sebba

IPC: G01N33/543 ,  B01L3/02 ,  B01L3/00

CPC classification number: G01N33/54333 ,  B01L3/0217 ,  B01L3/502 ,  B01L3/50825 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/10 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12M23/08 ,  C12M23/22 ,  C12M23/38 ,  C12M33/04 ,  C12M41/14 ,  C12M41/36 ,  G01N33/56911 ,  G01N35/0098

Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Abstract translation: 本文提供了用于检测和/或识别培养样品中的一种或多种特定微生物的方法，系统和装置。 与表面增强拉曼光谱（SERS）活性纳米颗粒相关联的指示剂颗粒，可与一种或多种感兴趣的微生物具有亲和性的一种或多种特异性结合成分，可与培养样品中的特定微生物形成复合物。 此外，也可以使用具有与一种或多种对一种或多种感兴趣的微生物具有亲和性的一种或多种特异性结合成员相关联的磁性捕获颗粒的捕获，以捕获微生物指示剂颗粒复合物并将复合物浓缩在测定容器的局部区域 用于随后的检测和识别。 复合物可以分散，沉淀和再分散，使得培养样品可以在孵育期间重复测试多次，以便实时监测培养物样品。