公开(公告)号：US09138460B2

公开(公告)日：2015-09-22

申请号：US13185472

申请日：2011-07-18

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: C12N5/077 ,  A61K38/18 ,  A61F2/30 ,  A61F2/44 ,  A61L27/38 ,  A61K45/06 ,  A61K35/32 ,  A61K35/33 ,  A61F2/32 ,  A61F2/38 ,  A61F2/40 ,  A61F2/28 ,  A61F2/46

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作用于饲养和生长活性核心组分的递送系统，而且还用作细胞分化的诱导剂。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US20110034959A1

公开(公告)日：2011-02-10

申请号：US12809145

申请日：2008-12-17

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier- Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier- Sevrain

IPC: A61B17/84

CPC classification number: A61B17/688 ,  Y10S606/903

Abstract: A cranial clamp for attaching a cranial flap to the skull comprises a clamp having a base to be placed against the lamina interna, and a cap acting as a locking member against the lamina externa. The clamp and the cap are linked together by an extension spring within the diploe through a burr hole or along/within the line of a craniotomy.

Abstract translation: 用于将颅骨瓣附接到颅骨的颅骨夹具包括夹具，其具有抵靠椎板内部的基部，以及用作抵抗椎板外部的锁定构件的帽。 夹具和盖子通过毛刺孔或沿着/在开颅手术线内部通过二倍体内的延伸弹簧连接在一起。

公开(公告)号：US09533024B2

公开(公告)日：2017-01-03

申请号：US12775771

申请日：2010-05-07

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: A61K38/18 ,  C12N5/00 ,  A61F2/30 ,  A61F2/44 ,  A61L27/38 ,  C12N5/077 ,  A61K45/06 ,  A61K35/32 ,  A61K35/33 ,  A61F2/32 ,  A61F2/38 ,  A61F2/40 ,  A61F2/28 ,  A61F2/46

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

公开(公告)号：US08298560B2

公开(公告)日：2012-10-30

申请号：US12775753

申请日：2010-05-07

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: A61K9/50 ,  A61F2/30 ,  A61F13/00

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作用于饲养和生长活性核心组分的递送系统，而且还用作细胞分化的诱导剂。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US07850983B2

公开(公告)日：2010-12-14

申请号：US11671082

申请日：2007-02-05

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: A61F2/30 ,  A61F13/00 ,  A61K9/50

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living, core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作饲养和生长活的核心组分，而且作为细胞分化诱导剂的递送系统。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US09545432B2

公开(公告)日：2017-01-17

申请号：US12775736

申请日：2010-05-07

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: C12N5/00 ,  A61K38/18 ,  A61F2/30 ,  A61F2/44 ,  A61L27/38 ,  C12N5/077 ,  A61K45/06 ,  A61K35/32 ,  A61K35/33 ,  A61F2/32 ,  A61F2/38 ,  A61F2/40 ,  A61F2/28 ,  A61F2/46

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作用于饲养和生长活性核心组分的递送系统，而且还用作细胞分化的诱导剂。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US08728495B2

公开(公告)日：2014-05-20

申请号：US12775720

申请日：2010-05-07

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: A61K9/00

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作用于饲养和生长活性核心组分的递送系统，而且还用作细胞分化的诱导剂。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US08491870B2

公开(公告)日：2013-07-23

申请号：US12809139

申请日：2008-11-23

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: A61K49/00

CPC classification number: A61K47/48723 ,  A61K31/70 ,  A61K38/363 ,  A61K38/39

Abstract: The present invention generally concerns the detection and/or treatment of aneurysm in a non-invasive manner. In particular cases, the invention concerns methods and compositions for localizing a labeled composition to the site of an aneurysm for its detection and, in further cases, treatment of the aneurysm. In specific cases, the composition targets a subendothelial component of the aneurysmal wall, such as a smooth muscle cell exposed at the luminal surface of the vessel. In further specific cases, the composition targets an integrin receptor or laminin.

Abstract translation: 本发明一般涉及以非侵入性方式检测和/或治疗动脉瘤。 在特定情况下，本发明涉及用于将标记的组合物定位到动脉瘤的位置用于其检测的方法和组合物，并且在另外的情况下涉及动脉瘤的治疗。 在特定情况下，组合物靶向动脉瘤壁的内皮下组分，例如暴露于血管腔表面的平滑肌细胞。 在更具体的情况下，组合物靶向整联蛋白受体或层粘连蛋白。

公开(公告)号：US20110275151A1

公开(公告)日：2011-11-10

申请号：US13185472

申请日：2011-07-18

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier-Sevrain

IPC: C12N5/071

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

Abstract translation: 描述了使用结合惰性结构和活性核的混合构建体来软骨生物修复的方法和组合物。 惰性结构不仅用作用于饲养和生长活性核心组分的递送系统，而且还用作细胞分化的诱导剂。 惰性结构包括同心的内部和外部和可充气/可膨胀的气球样生物聚合物。 生物核心包括由HDF组成的细胞 - 基质构建体，例如，种植在支架中。 该方法包括从患者手术切除损伤的软骨，并将混合构建体插入到上述外科手术后产生的空腔中。 惰性结构的气囊在目标区域内连续膨胀，例如接头。 本文还公开了通过机械应变将人成纤维细胞生长和分化成软骨细胞样细胞的方法。

公开(公告)号：US20100285093A1

公开(公告)日：2010-11-11

申请号：US12775771

申请日：2010-05-07

Applicant: Lionel C. Sevrain ,  Sylvie Y. Verdier- Sevrain

Inventor： Lionel C. Sevrain ,  Sylvie Y. Verdier- Sevrain

IPC: A61F2/28 ,  A61K35/32 ,  A61P19/04 ,  A61P43/00

CPC classification number: A61L27/3817 ,  A61F2/30756 ,  A61F2/3099 ,  A61F2/32 ,  A61F2/38 ,  A61F2/3804 ,  A61F2/40 ,  A61F2/441 ,  A61F2002/2817 ,  A61F2002/286 ,  A61F2002/30062 ,  A61F2002/30586 ,  A61F2002/30677 ,  A61F2002/30762 ,  A61F2002/30764 ,  A61F2002/30766 ,  A61F2002/444 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2002/4635 ,  A61F2210/0004 ,  A61K35/32 ,  A61K35/33 ,  A61K38/18 ,  A61K45/06 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2506/1307 ,  C12N2521/00 ,  C12N2533/74 ,  A61K2300/00

Abstract: Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.