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1.发明授权O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same 有权O-磷酸丝氨酸巯基酸酶突变体及其制备方法公开(公告)号：US09127324B2
公开(公告)日：2015-09-08
申请号：US13278100
申请日：2011-10-20
Applicant: Soo An Shin , Jin Sook Chang , Hye Won Um , Jae Hyun Jo , Byeong Cheol Song , Kyoung Min Lee
Inventor： Soo An Shin , Jin Sook Chang , Hye Won Um , Jae Hyun Jo , Byeong Cheol Song , Kyoung Min Lee
CPC classification number: C12Y205/01065 , C12N9/1085 , C12P13/12
Abstract: Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.
Abstract translation: 公开了一种O-磷酸丝氨酸二硫氢化酶（OPSS）突变体，其具有对应于SEQ ID NO：1的不含3至7个C末端氨基酸残基的分枝杆菌来源的氨基酸序列。 此外，公开了编码OPSS突变体的核酸分子，携带核酸分子的表达载体和用表达载体转化的转化体。 此外，提供了在OPSS突变体的存在下，使O-磷酸-L-丝氨酸（OPS）与硫化物反应的半胱氨酸的制造方法。 OPSS突变体具有改善的酶活性，可以通过简单的酶转化反应应用于环保生产L-半胱氨酸。
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公开(公告)号：US08524243B2
公开(公告)日：2013-09-03
申请号：US13618169
申请日：2012-09-14
Applicant: Hyang Choi , Soo An Shin , Si Yong Yang , Young Wook Cho
Inventor： Hyang Choi , Soo An Shin , Si Yong Yang , Young Wook Cho
IPC: A61K39/112 , C12N1/20
CPC classification number: A61K39/0275 , A61K2039/522 , A61K2039/552 , C07K14/255 , Y02A50/482
Abstract: The present invention relates to avirulent Salmonella Gallinarum variants by inactivating virulence gene clusters of Salmonella Gallinarum (SG), a main pathogen of avian salmonellosis, and various uses thereof notably in the production of Salmonella-specific lytic bacteriophages, pharmaceutical compositions and feed additives.
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3.发明授权Method of producing L-threonine using Escherichia coli strain with phosphoenolpyruvate carboxylase promoter replaced with cysteine synthase promoter 有权使用大肠杆菌菌株产生L-苏氨酸的方法用磷酸烯醇丙酮酸羧化酶启动子替换为半胱氨酸合成酶启动子公开(公告)号：US08486670B2
公开(公告)日：2013-07-16
申请号：US13658369
申请日：2012-10-23
Applicant: Kwang Ho Lee , Jae Yeong Ju , Ji Sun Lee , Young Bin Hwang , Sung Hoo Jhon , Eun Sung Koh , Chul Ha Kim , Soo An Shin
Inventor： Kwang Ho Lee , Jae Yeong Ju , Ji Sun Lee , Young Bin Hwang , Sung Hoo Jhon , Eun Sung Koh , Chul Ha Kim , Soo An Shin
IPC: C12P13/08
CPC classification number: C12P13/08 , C12N9/1085 , C12N9/88
Abstract: An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.
Abstract translation: 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。
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4.发明授权公开(公告)号：US08293515B2
公开(公告)日：2012-10-23
申请号：US12875863
申请日：2010-09-03
Applicant: Soo An Shin , Min Tae Park , Hyang Choi , Young Wook Cho , In Hye Kang , Su Jin Choi
Inventor： Soo An Shin , Min Tae Park , Hyang Choi , Young Wook Cho , In Hye Kang , Su Jin Choi
IPC: C12N7/00
CPC classification number: A61K35/76 , A01N63/00 , C12N7/00 , C12N2795/10321 , C12N2795/10332 , Y02A50/481 , Y02A50/483
Abstract: Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.
Abstract translation: 本文公开了一种新型噬菌体，其对一种或多种选自肠炎沙门氏菌，鼠伤寒沙门氏菌，沙门氏菌和沙门氏菌的沙门氏菌具有特异杀菌活性，而不影响有益细菌，除了显示出对酸，热的良好耐受性 和干燥。 新型噬菌体可广泛用作治疗剂，动物饲料或饮用水，清洁剂和消毒剂，用于预防和治疗肠炎沙门氏菌，鼠伤寒沙门氏菌，沙门氏菌或沙门氏菌沙门氏菌引起的传染病，包括沙门氏菌病，沙门氏菌食物中毒 ，家禽伤寒和Pullorum病或用于控制沙门氏菌。
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公开(公告)号：US20110052543A1
公开(公告)日：2011-03-03
申请号：US12875879
申请日：2010-09-03
Applicant: Soo An Shin , Min Tae Park , Hyang Choi , Young Wook Cho , In Hye Kang , Su Jin Choi
Inventor： Soo An Shin , Min Tae Park , Hyang Choi , Young Wook Cho , In Hye Kang , Su Jin Choi
CPC classification number: A61K35/76 , A01N63/00 , C12N7/00 , C12N2795/10321 , C12N2795/10332
Abstract: Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.
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Patent Agency Ranking
6.发明授权Microorganisms having improved ornithine-producing ability and method for producing ornithine using the same 有权具有提高的鸟氨酸生产能力的微生物及使用其生产鸟氨酸的方法公开(公告)号：US09303277B2
公开(公告)日：2016-04-05
申请号：US13992241
申请日：2011-12-08
Applicant: Hyang Choi , Kyoung Min Lee , Min Sun Kang , Sung Hoo Jhon , Hye Won Um , Su Jin Choi , Han Won Lee , Soo An Shin
Inventor： Hyang Choi , Kyoung Min Lee , Min Sun Kang , Sung Hoo Jhon , Hye Won Um , Su Jin Choi , Han Won Lee , Soo An Shin
CPC classification number: C12P13/10 , C07K14/34 , C12N9/0008 , C12N9/1018 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12R1/15 , C12Y102/01038 , C12Y201/03003 , C12Y203/01035 , C12Y206/01013 , C12Y207/02008
Abstract: The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.
Abstract translation: 本发明涉及具有改善的鸟氨酸生产能力的微生物，其中精氨酸形成鸟氨酸的生物合成途径被阻断，细胞内谷氨酸水平提高，鸟氨酸从谷氨酸生物合成途径增强， 鸟氨酸使用微生物。
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7.发明授权Method for separating and purifying 1,4-diaminobutane from fermented solution 有权从发酵液中分离纯化1,4-二氨基丁烷的方法公开(公告)号：US08927774B2
公开(公告)日：2015-01-06
申请号：US14240695
申请日：2012-08-24
Applicant: Won Sik Gwak , Soon Won Hong , Soo An Shin , Han Won Lee
Inventor： Won Sik Gwak , Soon Won Hong , Soo An Shin , Han Won Lee
IPC: C07C209/00 , C07C209/86 , C12P13/00 , C07C209/84
CPC classification number: C07C209/86 , C07C209/84 , C12P13/001 , C07C211/09
Abstract: The present invention relates to a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution comprising 1,4-diaminobutane, through cell mass removement, desalination, concentration, impurities removal, and recovery. Also, provided is a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution 1,4-diaminobutane, through cell mass removement, desalination, low-temperature concentration, crystallization, filtration, high-temperature concentration and distillation.
Abstract translation: 本发明涉及从包含1,4-二氨基丁烷的发酵溶液中通过细胞去除，脱盐，浓缩，杂质去除和回收，以高纯度和高收率分离和纯化1,4-二氨基丁烷的方法。 此外，提供了从发酵液1,4-二氨基丁烷分离纯化和高产率的1,4-二氨基丁烷的方法，通过细胞质量去除，脱盐，低温浓缩，结晶，过滤，高温 浓缩和蒸馏。
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8.发明申请METHOD FOR SEPARATING AND PURIFYING 1,4-DIAMINOBUTANE FROM FERMENTED SOLUTION 有权从发泡溶液中分离和纯化1,4-二氨基丁酸的方法公开(公告)号：US20140213824A1
公开(公告)日：2014-07-31
申请号：US14240695
申请日：2012-08-24
Applicant: Won Sik Gwak , Soon Won Hong , Soo An Shin , Han Won Lee
Inventor： Won Sik Gwak , Soon Won Hong , Soo An Shin , Han Won Lee
IPC: C07C209/86 , C07C209/84
CPC classification number: C07C209/86 , C07C209/84 , C12P13/001 , C07C211/09
Abstract: The present invention relates to a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution comprising 1,4-diaminobutane, through cell mass removement, desalination, concentration, impurities removal, and recovery. Also, provided is a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution 1,4-diaminobutane, through cell mass removement, desalination, low-temperature concentration, crystallization, filtration, high-temperature concentration and distillation.
Abstract translation: 本发明涉及从包含1,4-二氨基丁烷的发酵溶液中通过细胞去除，脱盐，浓缩，杂质去除和回收，以高纯度和高收率分离和纯化1,4-二氨基丁烷的方法。 此外，提供了从发酵液1,4-二氨基丁烷分离纯化和高产率的1,4-二氨基丁烷的方法，通过细胞质量去除，脱盐，低温浓缩，结晶，过滤，高温 浓缩和蒸馏。
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9.发明申请MICROORGANISM PRODUCING O-PHOSPHOSERINE AND METHOD OF PRODUCING L-CYSTEINE OR DERIVATIVES THEREOF FROM O-PHOSPHOSERINE USING THE SAME 有权生产O-磷酸的微生物及其使用其的O-磷酸酯生产L-胞嘧啶或其衍生物的方法公开(公告)号：US20120190083A1
公开(公告)日：2012-07-26
申请号：US13278106
申请日：2011-10-20
Applicant: Jin Sook CHANG , Jae Hyun JO , Hyun Ae BAE , Byeong Cheol SONG , Sol KIM , Hye Won KIM , Hye Won UM , Sung Hoo JHON , Yong Uk SHIN , Eun Bin YANG , Kyoung Min LEE , Soo An SHIN
Inventor： Jin Sook CHANG , Jae Hyun JO , Hyun Ae BAE , Byeong Cheol SONG , Sol KIM , Hye Won KIM , Hye Won UM , Sung Hoo JHON , Yong Uk SHIN , Eun Bin YANG , Kyoung Min LEE , Soo An SHIN
CPC classification number: C12P13/12 , C12N9/1085 , C12N9/16 , C12N15/70 , C12N15/77 , C12P13/06 , C12Y205/01065 , C12Y301/03003
Abstract: The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and the activity of PhnC, PhnD, and PhnE is reduced, and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having these reduced and enhanced properties noted above are also provided herein.
Abstract translation: 本发明提供了通过培养具有降低的内源性磷酸丝氨酸磷酸酶活性的微生物来生产半胱氨酸或其衍生物的方法，并且PhnC，PhnD和PhnE的活性降低，并且提高了磷酸甘油酸脱氢酶和/或磷酸丝氨酸氨基转移酶的活性。 由这样的生物体产生的O-磷酸丝氨酸然后可以在硫酸酯或表达巯基化酶的微生物的存在下与硫化物反应，以产生半胱氨酸或其衍生物。 本文还提供了具有上述这些降低和增强性能的微生物。
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10.发明申请ESCHERICHIA COLI STRAIN WITH ENHANCED L-THREONINE PRODUCTIVITY AND METHOD OF PRODUCING L-THREONINE USING THE SAME 有权具有增强的L-苏氨酸生产力的ESCHERICHIA COLI菌株和使用该柠檬酸生产L-赖氨酸的方法公开(公告)号：US20110189739A1
公开(公告)日：2011-08-04
申请号：US12812203
申请日：2009-01-07
Applicant: Kwang Ho Lee , Jae Yeong Ju , Ji Sun Lee , Young Bin Hwang , Sung Hoo Jhon , Eun Sung Koh , Chul Ha Kim , Soo An Shin
Inventor： Kwang Ho Lee , Jae Yeong Ju , Ji Sun Lee , Young Bin Hwang , Sung Hoo Jhon , Eun Sung Koh , Chul Ha Kim , Soo An Shin
CPC classification number: C12P13/08 , C12N9/1085 , C12N9/88
Abstract: An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.
Abstract translation: 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。
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