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    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE
    3.
    发明申请
    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE 审中-公开
    荧光同位素标记及其使用方法

    公开(公告)号：US20110171736A1

    公开(公告)日：2011-07-14

    申请号：US12944316

    申请日：2010-11-11

    Applicant: Brian AGNEW ,  Kyle Richard Gee

    Inventor： Brian AGNEW ,  Kyle Richard Gee

    IPC: G01N33/50 ,  C07D207/46 ,  C07D311/88 ,  C07D405/12 ,  C07D235/02

    CPC classification number: G01N33/582 ,  C09B11/24 ,  G01N33/533 ,  G01N33/534 ,  G01N33/583 ,  G01N33/60 ,  G01N33/6848

    Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.

    Abstract translation: 本发明提供了引入稳定同位素（氘，13-碳，15-氮，18-氧）取代的新型反应性荧光化合物。 本发明包括将这些化合物与非同位素取代的类似物组合用于通过液相色谱（LC）和质谱法的组合来纯化，鉴定和相对定量蛋白质，肽，糖，代谢物和其它生物学上重要的化合物 （女士）。 目标化合物的荧光标记以这种方式提供超过传统稳定同位素标记的数量级的灵敏度增强，并且由于不同荧光团的多波长性质，也提供同时多重分析的可能性。

    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE
    5.
    发明申请
    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE 审中-公开
    荧光同位素标记及其使用方法

    公开(公告)号：US20090017546A1

    公开(公告)日：2009-01-15

    申请号：US12177057

    申请日：2008-07-21

    Applicant: Brian Agnew ,  Kyle Richard Gee

    Inventor： Brian Agnew ,  Kyle Richard Gee

    IPC: G01N33/68 ,  C07D405/12 ,  C07D495/04

    CPC classification number: G01N33/582 ,  C09B11/24 ,  G01N33/533 ,  G01N33/534 ,  G01N33/583 ,  G01N33/60 ,  G01N33/6848

    Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.

    Abstract translation: 本发明提供了引入稳定同位素（氘，13-碳，15-氮，18-氧）取代的新型反应性荧光化合物。 本发明包括将这些化合物与非同位素取代的类似物组合用于通过液相色谱（LC）和质谱法的组合来纯化，鉴定和相对定量蛋白质，肽，糖，代谢物和其它生物学上重要的化合物 （女士）。 目标化合物的荧光标记以这种方式提供超过传统稳定同位素标记的数量级的灵敏度增强，并且由于不同荧光团的多波长性质，也提供同时多重分析的可能性。

    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE
    7.
    发明申请
    FLUORESCENT ISOTOPE TAGS AND THEIR METHOD OF USE 审中-公开
    荧光同位素标记及其使用方法

    公开(公告)号：US20100081203A1

    公开(公告)日：2010-04-01

    申请号：US12487945

    申请日：2009-06-19

    Applicant: Brian Agnew ,  Kyle Richard Gee

    Inventor： Brian Agnew ,  Kyle Richard Gee

    IPC: G01N33/48 ,  C07D403/12 ,  C07D311/82 ,  C07D405/12 ,  C07D235/02

    CPC classification number: G01N33/582 ,  C09B11/24 ,  G01N33/533 ,  G01N33/534 ,  G01N33/583 ,  G01N33/60 ,  G01N33/6848

    Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.

    Abstract translation: 本发明提供了引入稳定同位素（氘，13-碳，15-氮，18-氧）取代的新型反应性荧光化合物。 本发明包括将这些化合物与非同位素取代的类似物组合用于通过液相色谱（LC）和质谱法的组合来纯化，鉴定和相对定量蛋白质，肽，糖，代谢物和其它生物学上重要的化合物 （女士）。 目标化合物的荧光标记以这种方式提供超过传统稳定同位素标记的数量级的灵敏度增强，并且由于不同荧光团的多波长性质，也提供同时多重分析的可能性。

    SITE-SPECIFIC LABELING OF AFFINITY PEPTIDES IN FUSION PROTEINS
    8.
    发明申请
    SITE-SPECIFIC LABELING OF AFFINITY PEPTIDES IN FUSION PROTEINS 审中-公开
    融合蛋白中的亲和肽的特异性标记

    公开(公告)号：US20090004641A1

    公开(公告)日：2009-01-01

    申请号：US11871031

    申请日：2007-10-11

    Applicant: Kyle Richard Gee ,  Courtenay Rae Hart ,  Richard Haugland ,  Wayne Forrest Patton ,  Scott Whitney

    Inventor： Kyle Richard Gee ,  Courtenay Rae Hart ,  Richard Haugland ,  Wayne Forrest Patton ,  Scott Whitney

    IPC: C12Q1/00 ,  C07D401/14 ,  C07D207/30

    CPC classification number: C07D263/62 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07D405/14 ,  C07F5/022 ,  G01N1/30 ,  G01N33/533 ,  G01N33/582

    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

    Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

    Competitive immunoassay
    10.
    发明授权
    Competitive immunoassay 有权
    竞争性免疫测定

    公开(公告)号：US07282339B2

    公开(公告)日：2007-10-16

    申请号：US10943463

    申请日：2004-09-17

    Applicant: Joseph Beechem ,  Kyle Richard Gee ,  David Carl Hagen ,  Iain D Johnson ,  Hee-Chol Kang ,  Christina Pastula

    Inventor： Joseph Beechem ,  Kyle Richard Gee ,  David Carl Hagen ,  Iain D Johnson ,  Hee-Chol Kang ,  Christina Pastula

    IPC: G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  C12Q1/42 ,  C07K1/13

    CPC classification number: G01N33/542 ,  C07F9/12 ,  C07F9/65318 ,  C07F9/65522 ,  C07F9/6596 ,  C07K16/46 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S530/807 ,  Y10T436/13

    Abstract: The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.

    Abstract translation: 本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。 配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。 该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。 取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于 样品。 或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。 在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。

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