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1.发明授权Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 失效来自Thermus物种Z05的突变的热稳定性核酸聚合酶
公开(公告)号：US5455170A
公开(公告)日：1995-10-03
申请号：US113531
申请日：1993-08-27
Applicant: Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
Inventor： Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
CPC classification number: C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
Abstract: A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Abstract translation: 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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2.发明授权DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 失效编码来自热带菌种sps17的突变的热稳定性核酸聚合酶的DNA
公开(公告)号：US5405774A
公开(公告)日：1995-04-11
申请号：US119754
申请日：1993-09-10
Applicant: Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
Inventor： Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
CPC classification number: C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
Abstract: A purified thermostable enzyme is derived from the bacterium Thermus species sps17. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally, 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and can be used with selected primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Abstract translation: 纯化的热稳定酶源自嗜热菌菌种sps17。 该酶具有DNA聚合酶活性，逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与选定的引物和三磷酸核苷一起使用，其中至少一个核酸序列的量从现有的序列扩增。
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公开(公告)号：US20120040380A1
公开(公告)日：2012-02-16
申请号：US13166627
申请日：2011-06-22
Applicant: SCHUYLER BOON CORRY , WILLIAM LOUIS DOWNEY , BRIAN FILANOSKI , KYLE RICHARD GEE , I. LAWRENCE GREENFIELD , JAMES DAVID HIRSCH , IAIN JOHNSON , ALEKSEY RUKAVISHNIKOV
Inventor： SCHUYLER BOON CORRY , WILLIAM LOUIS DOWNEY , BRIAN FILANOSKI , KYLE RICHARD GEE , I. LAWRENCE GREENFIELD , JAMES DAVID HIRSCH , IAIN JOHNSON , ALEKSEY RUKAVISHNIKOV
CPC classification number: C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
Abstract translation: 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。
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公开(公告)号：US20090047692A1
公开(公告)日：2009-02-19
申请号：US12099085
申请日：2008-04-07
Applicant: Schuyler Boon CORRY , William Louis Downey , Brian Filanoski , Kyle Richard Gee , I. Lawrence Greenfield , James David Hirsch , Iain Johnson , Aleksey Rukavishnikov
Inventor： Schuyler Boon CORRY , William Louis Downey , Brian Filanoski , Kyle Richard Gee , I. Lawrence Greenfield , James David Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC: G01N33/53 , C07D501/60 , C07D498/04 , C12Q1/34
CPC classification number: C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
Abstract translation: 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中存在β-内酰胺酶的方法，例如作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。
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5.发明授权
公开(公告)号：US5968799A
公开(公告)日：1999-10-19
申请号：US977428
申请日：1993-02-09
Applicant: David H. Gelfand , I. Lawrence Greenfield , Fred L. Reichert
Inventor： David H. Gelfand , I. Lawrence Greenfield , Fred L. Reichert
CPC classification number: C12N9/1252
Abstract: A purified thermostable enzyme is derived from the eubacterium Thermosipho africanus. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' and/or 3'.fwdarw.5' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Abstract translation: PCT No.PCT / US91 / 07076 Sec。 371日期：1993年2月9日 102（e）日期1993年2月9日PCT 1991年9月26日PCT公布。 出版物WO92 / 06202 日期：1992年4月16日纯化的热稳定酶源自热带杆菌（Thermosipho africanus）。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'和/或3'→5'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列的量从现有序列中扩增。
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Patent Agency Ranking6.发明授权Coupling agents and sterically hindered disulfide linked conjugates prepared therefrom 失效偶联剂和由其制备的空间位阻二硫键连接的共轭物
公开(公告)号：US5241078A
公开(公告)日：1993-08-31
申请号：US837678
申请日：1992-02-14
Applicant: Margaret Moreland , I. Lawrence Greenfield , Danute E. Nitecki
Inventor： Margaret Moreland , I. Lawrence Greenfield , Danute E. Nitecki
IPC: C07C327/32 , C07D207/44 , C07D207/452 , C07D207/46 , G01N33/532
CPC classification number: C07D207/452 , C07C327/32 , C07D207/46 , G01N33/532
Abstract: This invention discloses heterobifunctional coupling agents for making a wide array of molecular conjugates. The agents contain a sterically hindered thiol, linked through a spacer arm to a second group reactive toward nucleophiles such as 1.degree. and 2.degree. amines or reactive thiols that are present on biological and organic materials. The coupling agents are useful for making conjugates containing a sterically hindered linkage.
Abstract translation: 本发明公开了用于制备广泛分子结合物的异双功能偶联剂。 这些试剂含有空间位阻硫醇，其通过间隔臂连接到对亲核试剂反应的第二组，所述亲核试剂例如存在于生物和有机材料上的1℃和2℃的胺或反应性硫醇。 偶联剂可用于制备含空间位阻键的缀合物。
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公开(公告)号：US07217518B2
公开(公告)日：2007-05-15
申请号：US10628879
申请日：2003-07-28
Applicant: Michael M. A. Sekar , I. Lawrence Greenfield
Inventor： Michael M. A. Sekar , I. Lawrence Greenfield
IPC: C12Q1/68
CPC classification number: G01N33/582 , C12Q1/6837 , G01N33/54313 , C12Q2561/119 , C12Q2525/205
Abstract: The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.
Abstract translation: 本发明涉及通过测量分析物与特定适体的结合来测量荧光各向异性的变化来检测样品中一种或多种目标分析物的存在的方法。 适体被固定在固体支持物上并且可以是阵列的形式。
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8.发明授权DNA encoding thermostable nucleic acid polymerase enzyme from thermus species Z05 失效从热带菌Z05编码热稳定性核酸聚合酶的DNA
公开(公告)号：US5674738A
公开(公告)日：1997-10-07
申请号：US520422
申请日：1995-08-29
Applicant: Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
Inventor： Richard D. Abramson , David H. Gelfand , I. Lawrence Greenfield
CPC classification number: C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
Abstract: A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Abstract translation: 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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9.发明授权Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases 有权使用表面活性剂和蛋白酶分离核酸的组合物，方法和试剂盒公开(公告)号：US07001724B1
公开(公告)日：2006-02-21
申请号：US09724613
申请日：2000-11-28
Applicant: I. Lawrence Greenfield
Inventor： I. Lawrence Greenfield
CPC classification number: C12N15/1006
Abstract: The invention provides compositions and methods for releasing and for isolating nucleic acids from biological samples, preferably from whole tissue, using cationic surfactants and proteases. The surfactant-protease combinations, when used with whole tissue, macerate the tissue, lyse individual cells, release nucleic acids, and inactivate nucleases. Kits for isolating nucleic acids from biological samples, particularly from whole tissue, are also provided.
Abstract translation: 本发明提供了使用阳离子表面活性剂和蛋白酶释放和分离来自生物样品，优选来自整个组织的核酸的组合物和方法。 当与整个组织一起使用时，表面活性剂 - 蛋白酶组合浸没组织，裂解个体细胞，释放核酸和灭活核酸酶。 还提供了用于从生物样品，特别是从整个组织分离核酸的试剂盒。
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10.发明授权
公开(公告)号：US4933288A
公开(公告)日：1990-06-12
申请号：US934248
申请日：1986-11-21
Applicant: I. Lawrence Greenfield
Inventor： I. Lawrence Greenfield
Abstract: DNA sequences encoding proteins processable by secretion leaders in recombinant hosts are described. The DNA sequences encode the NH.sub.2 -terminal region of proteins that are cleaved from a secretion leader and may be secreted through the cell membrane and, if present, cell wall in some cases. Proteins encoded by the DNA sequence have an NH.sub.2 -terminal amino acid sequence conforming to a consensus amino acid sequence that is processable by the particular secretion leader. DNA sequences encoding a consensus amino acid sequence processable by the diphterhia toxin secretion leader are disclosed. A novel Pseudomonas exotoxin and CSF-1 having NH.sub.2 -terminal sequences conforming to a consensus sequence are exemplified.
Abstract translation: 描述了编码可由重组宿主中分泌前导序列加工的蛋白质的DNA序列。 DNA序列编码从分泌前导部分切割的蛋白质的NH2-末端区域，并且在某些情况下可以通过细胞膜分泌细胞壁（如果存在）。 由DNA序列编码的蛋白质具有符合可由特定分泌前导序列加工的共有氨基酸序列的NH2末端氨基酸序列。 公开了编码可由二倍体毒素分泌前导序列加工的共有氨基酸序列的DNA序列。 例示了具有符合共有序列的NH2末端序列的新型假单胞菌外毒素和CSF-1。
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