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    Novel nitrile hydratase
    1.
    发明申请
    Novel nitrile hydratase 有权
    新型腈水合酶

    公开(公告)号：US20070009985A1

    公开(公告)日：2007-01-11

    申请号：US10539560

    申请日：2003-12-15

    申请人： Toshifumi Yamaki ,  Shinichi Banba ,  Kaori Matoishi ,  Kiyoshi Ito ,  Hideki Kobayashi ,  Eishi Tanaka ,  Toshihiro Oikawa

    发明人： Toshifumi Yamaki ,  Shinichi Banba ,  Kaori Matoishi ,  Kiyoshi Ito ,  Hideki Kobayashi ,  Eishi Tanaka ,  Toshihiro Oikawa

    IPC分类号： C12P21/06 ,  C12P13/00 ,  C07H21/04 ,  C12N9/78 ,  C12N1/21

    CPC分类号： C12Y402/01084 ,  C12N9/88

    摘要： The amino acid sequence of a mutant which is obtained by introducing a novel mutation into a Pseudonocardia thermophila JCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.

    摘要翻译： 提供了通过将新突变引入由两种异质亚基组成的嗜热假诺卡氏菌JCM3095衍生的腈水合酶获得的突变体的氨基酸序列和该基因的碱基序列。 通过在腈水合酶的立体结构/氨基酸序列中指定待修饰的区域，并将替代，插入，缺失等的改变应用于氨基酸序列中相应的氨基酸序列中的氨基酸来修饰腈水合酶 到形成该区域的氨基酸残基。 还提供了修饰具有腈水合酶活性的酶的方法。

    MOLDED ARTICLE
    2.
    发明申请
    MOLDED ARTICLE 有权
    模制品

    公开(公告)号：US20130344273A1

    公开(公告)日：2013-12-26

    申请号：US13980950

    申请日：2012-01-18

    申请人： Kaori Matoishi ,  Takuma Yano ,  Hiromasa Marubayashi ,  Naoki Tomoshige ,  Naoshi Nagai ,  Tamotsu Harada ,  Kuniaki Kawabe

    发明人： Kaori Matoishi ,  Takuma Yano ,  Hiromasa Marubayashi ,  Naoki Tomoshige ,  Naoshi Nagai ,  Tamotsu Harada ,  Kuniaki Kawabe

    IPC分类号： C08L23/26

    CPC分类号： C08L23/26 ,  C08F8/42 ,  C08F10/00 ,  C08F110/02 ,  C08F2810/40 ,  C08L23/10 ,  C08L83/00 ,  C08L83/04 ,  C08L101/10 ,  C08L2312/08 ,  Y10T428/1397 ,  Y10T428/249921 ,  C08L51/06

    摘要： A molded article formed of a composition including 100 parts by weight of at least one kind of resin selected from the group consisting of thermoplastic resins and thermosetting resins, and 0.01 parts by weight to 10,000 parts by weight of a silylated polyolefin, which is obtained by a reaction between a silicon-containing compound containing a structural unit represented by Formula (1) and a vinyl group-containing compound having a defined number average molecular weight, a derivative thereof, or a mixture of these, —Si(R1)H—Y1—  (1) wherein R1 and Y1 are defined and with the proviso that the silylated polyolefin is not obtained by a reaction between a silicon-containing compound containing a structural unit represented by Formula (1) and having two or more SH groups per molecule and a compound having 2.0 or more vinyl groups on average per molecule as the vinyl group-containing compound.

    摘要翻译： 由包含100重量份的选自热塑性树脂和热固性树脂的至少一种树脂和0.01重量份至10,000重量份的甲硅烷基化聚烯烃的组合物形成的模塑制品，其通过 包含式（1）表示的结构单元的含硅化合物和具有确定数均分子量的含乙烯基的化合物，其衍生物或它们的混合物之间的反应-Si（R1）H- Y 1 - （1）其中R1和Y1被定义，条件是甲硅烷基化聚烯烃不是通过含有由式（1）表示的结构单元的含硅化合物与每分子具有两个或更多个SH基团的反应而得到的 和每分子平均具有2.0个以上乙烯基的化合物作为含乙烯基的化合物。

    Nitrile hydratase variant
    4.
    发明授权
    Nitrile hydratase variant 有权
    腈水合酶变体

    公开(公告)号：US07595184B2

    公开(公告)日：2009-09-29

    申请号：US10539560

    申请日：2003-12-15

    申请人： Toshifumi Yamaki ,  Shinichi Banba ,  Kaori Matoishi ,  Kiyoshi Ito ,  Hideki Kobayashi ,  Eishi Tanaka ,  Toshihiro Oikawa

    发明人： Toshifumi Yamaki ,  Shinichi Banba ,  Kaori Matoishi ,  Kiyoshi Ito ,  Hideki Kobayashi ,  Eishi Tanaka ,  Toshihiro Oikawa

    IPC分类号： C12N9/88 ,  C12N5/10 ,  C12N1/21 ,  C07K14/00 ,  C12P21/00 ,  C12N15/00 ,  C12Q1/527 ,  C07H21/00

    CPC分类号： C12Y402/01084 ,  C12N9/88

    摘要： The amino acid sequence of a mutant which is obtained by introducing a novel mutation into a Pseudonocardia thermophila JCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.

    摘要翻译： 提供了通过将新突变引入由两种异质亚基组成的嗜热假诺卡氏菌JCM3095衍生的腈水合酶获得的突变体的氨基酸序列和该基因的碱基序列。 通过在腈水合酶的立体结构/氨基酸序列中指定待修饰的区域，并将替代，插入，缺失等的改变应用于氨基酸序列中相应的氨基酸序列中的氨基酸来修饰腈水合酶 到形成该区域的氨基酸残基。 还提供了修饰具有腈水合酶活性的酶的方法。

    Molded article
    9.
    发明授权
    Molded article 有权
    成型品

    公开(公告)号：US09534112B2

    公开(公告)日：2017-01-03

    申请号：US13980950

    申请日：2012-01-18

    申请人： Kaori Matoishi ,  Takuma Yano ,  Hiromasa Marubayashi ,  Naoki Tomoshige ,  Naoshi Nagai ,  Tamotsu Harada ,  Kuniaki Kawabe

    发明人： Kaori Matoishi ,  Takuma Yano ,  Hiromasa Marubayashi ,  Naoki Tomoshige ,  Naoshi Nagai ,  Tamotsu Harada ,  Kuniaki Kawabe

    IPC分类号： C08L23/26 ,  C08L83/08 ,  C08L101/10 ,  C08L83/00 ,  C08L83/04 ,  C08F110/02 ,  C08F10/00

    CPC分类号： C08L23/26 ,  C08F8/42 ,  C08F10/00 ,  C08F110/02 ,  C08F2810/40 ,  C08L23/10 ,  C08L83/00 ,  C08L83/04 ,  C08L101/10 ,  C08L2312/08 ,  Y10T428/1397 ,  Y10T428/249921 ,  C08L51/06

    摘要： A molded article formed of a composition including 100 parts by weight of at least one kind of resin selected from the group consisting of thermoplastic resins and thermosetting resins, and 0.01 parts by weight to 10,000 parts by weight of a silylated polyolefin, which is obtained by a reaction between a silicon-containing compound containing a structural unit represented by Formula (1) and a vinyl group-containing compound having a defined number average molecular weight, a derivative thereof, or a mixture of these, —Si(R1)H—Y1—  (1) wherein R1 and Y1 are defined and with the proviso that the silylated polyolefin is not obtained by a reaction between a silicon-containing compound containing a structural unit represented by Formula (1) and having two or more SH groups per molecule and a compound having 2.0 or more vinyl groups on average per molecule as the vinyl group-containing compound.

    摘要翻译： 本发明的成形品是将由热塑性树脂和热固化性树脂组成的组中选出的至少一种树脂100重量份和0.01重量份〜10,000重量份的 通过含有式（1）表示的结构单元的含硅化合物与数均分子量等于或大于100且等于或等于或等于或等于100的含乙烯基的化合物之间的反应获得的甲硅烷基化聚烯烃， 通过GPC测量的小于500,000（这里，使用每分子具有2个以上SiH基的化合物作为含硅化合物的情况和每分子平均具有2.0个以上乙烯基的化合物作为含乙烯基的情况 化合物），其衍生物，或它们的混合物。 ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ-Si（R 1）HY 1 - €ƒ€ƒ€ƒ（1）（在公式（1）中，R 1表示氢 原子，卤原子或烃基，Y 1表示O，S或NR 30（R 30表示氢原子或烃基）。