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公开(公告)号：US08808643B1
公开(公告)日：2014-08-19
申请号：US13228391
申请日：2011-09-08
Applicant: W. Henry Benner , John M. Dzenitis , William J Benett , Brian R. Baker
Inventor： W. Henry Benner , John M. Dzenitis , William J Benett , Brian R. Baker
IPC: B01L3/02
CPC classification number: B01L3/502715 , B01L3/502738 , B01L2300/0864 , B01L2400/0622
Abstract: Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.
Abstract translation: 本文提供了流动平台和样品制备和分析方法。 流体学平台能够使用诸如聚合酶链反应测定和环介导等温扩增测定之类的扩增测定法从血液样品中分析DNA。 流体学平台也可用于其他类型的测定和分析。 在一些实施例中，密封管中的样品可以直接插入。 无需用户干预即可执行以下隔离，检测和分析。 所公开的平台还可以包括具有磁性致动器，加热器和空气干燥机构的样品制备系统，以及用于在反应之后和样品之间的萃取，洗涤，洗脱，测定组装，测定检测和清洁的流体操纵过程。
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公开(公告)号：US20090209030A1
公开(公告)日：2009-08-20
申请号：US12415623
申请日：2009-03-31
Applicant: William J. Benett , James B. Richards , Paul L. Stratton , Elizabeth K. Wheeler , Peter Krulevitch , Steve Visuri , John M. Dzenitis
Inventor： William J. Benett , James B. Richards , Paul L. Stratton , Elizabeth K. Wheeler , Peter Krulevitch , Steve Visuri , John M. Dzenitis
CPC classification number: B01L7/52 , B01L3/5082 , B01L2200/12 , B01L2300/123 , B01L2300/1827 , B01L2300/1844 , Y10T29/49124
Abstract: A thermalcycler is constructing with a first thermalcycler body section of a flexible circuit material or a circuit board material. The first thermalcycler body section has a first face. A first cavity portion is formed in the first face. A second thermalcycler body section is constructed of a flexible circuit material or a circuit board material. The second thermalcycler body section has a second face. A second cavity portion is formed in the second face. When the first cavity portion and the second cavity portion are positioned together they form a cavity. A thermalcycler unit is positioned in the cavity. The first thermalcycler body section and the second thermalcycler body section are connected together with the first face and the face opposed to each other and the thermalcycler unit operatively connected to the first cavity portion and the second cavity portion.
Abstract translation: 热循环器正在构造具有柔性电路材料或电路板材料的第一热循环体主体部分。 第一热循环体主体部分具有第一面。 第一空腔部分形成在第一面中。 第二个热循环器主体部分由柔性电路材料或电路板材料构成。 第二热循环器本体部分具有第二面。 第二空腔部分形成在第二面中。 当第一空腔部分和第二空腔部分位于一起时，它们形成空腔。 热循环器单元位于空腔中。 第一热循环器主体部分和第二热循环器主体部分与第一面和彼此相对的面连接在一起，并且热循环器单元可操作地连接到第一腔部分和第二腔部分。
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3.发明申请Systems and Methods for Separating Particles and/or Substances from a Sample Fluid 有权从样品流体中分离颗粒和/或物质的系统和方法公开(公告)号：US20090194420A1
公开(公告)日：2009-08-06
申请号：US12024762
申请日：2008-02-01
Applicant: Raymond P. Mariella, JR. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
Inventor： Raymond P. Mariella, JR. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
CPC classification number: B01D17/06 , G01N1/4077 , G01N2001/4072 , G01N2015/0288
Abstract: Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chambers the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.
Abstract translation: 用于从样品流体中分离颗粒和/或毒素的系统和方法。 根据一个实施例的方法包括同时通过样品流体和缓冲流体通过室，使得当流体通过腔室时，在样品流体和缓冲流体之间形成流体界面，其中具有感兴趣的颗粒的样品流体; 向流体施加力以促使感兴趣的颗粒通过界面进入缓冲流体; 并且从样品流体基本上分离缓冲流体。
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公开(公告)号：US07413711B2
公开(公告)日：2008-08-19
申请号：US10621657
申请日：2003-07-16
Applicant: William J. Benett , Peter A. Krulevitch , Steven R. Visuri , John M. Dzenitis , Kevin D. Ness
Inventor： William J. Benett , Peter A. Krulevitch , Steven R. Visuri , John M. Dzenitis , Kevin D. Ness
IPC: B01L3/02
CPC classification number: B01L3/0217 , B01L2400/0487 , G01N35/1016 , G01N35/1065 , G01N2035/1034
Abstract: A dispensing system delivers a precise amount of fluid for biological or chemical processing and/or analysis. Dispensing means moves the fluid. The dispensing means is operated by a pneumatic force. Connection means delivers the fluid to the desired location. An actuator means provides the pneumatic force to the dispensing means. Valving means transmits the pneumatic force from the actuator means to the dispensing means.
Abstract translation: 分配系统提供精确量的用于生物或化学处理和/或分析的流体。 分液装置移动流体。 分配装置通过气动力操作。 连接装置将流体输送到所需位置。 致动器装置向分配装置提供气动力。 阀装置将气动力从致动器装置传递到分配装置。
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公开(公告)号：US20080025871A1
公开(公告)日：2008-01-31
申请号：US11495952
申请日：2006-07-27
Applicant: John M. Dzenitis , Benjamin J. Hindson , Anthony J. Makarewicz , William J. Benett , Aaron M. Mason , Paul B. Haigh
Inventor： John M. Dzenitis , Benjamin J. Hindson , Anthony J. Makarewicz , William J. Benett , Aaron M. Mason , Paul B. Haigh
CPC classification number: B01F11/0022 , B01F11/0065 , B01F11/0071 , B01F11/0074 , B01F15/00831 , B01F15/0085 , G01N35/1002
Abstract: A system for maintaining a liquid slurry of microbeads for use in biological or chemical analysis comprising providing a flexible bag and filling the flexible bag with a slurry of a liquid and microbeads. One embodiment is an apparatus for maintaining liquid slurries of particles for use in biological or chemical analysis comprising a bag, a liquid contained within the bag, and particles contained within the liquid in the bag.
Abstract translation: 一种用于维持用于生物或化学分析的微珠的液体浆料的系统，包括提供柔性袋，并用液体和微珠的浆液填充柔性袋。 一个实施方案是一种用于维持用于生物或化学分析的液体浆液的装置，其包括袋，包含在袋内的液体和包含在袋中的液体中的颗粒。
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Patent Agency Ranking
公开(公告)号：US08778663B2
公开(公告)日：2014-07-15
申请号：US11901787
申请日：2007-09-18
Applicant: William J. Benett , John T. Andreski , John M. Dzenitis , Anthony J. Makarewicz , Dean R. Hadley , Satinderpall S. Pannu
Inventor： William J. Benett , John T. Andreski , John M. Dzenitis , Anthony J. Makarewicz , Dean R. Hadley , Satinderpall S. Pannu
CPC classification number: B01L7/52 , B01L3/50851 , B01L7/525 , B01L2200/02 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , C12M41/14 , C12Q1/6844 , Y10T29/4935
Abstract: A thermalcycler includes a first thermalcycler body section having a first face and a second thermalcycler body section having a second face. A cavity is formed by the first face and the second face. A thermalcycling unit is positioned in the cavity. A heater trace unit is connected to a support section, to the first thermalcycler body section, to the second thermalcycler body section, and to the thermalcycling unit. The first thermalcycler body section and the second thermalcycler body section are positioned together against the support section to enclose the thermalcycling unit and the heater trace unit.
Abstract translation: 热循环器包括具有第一面的第一热循环器主体部分和具有第二面的第二热循环器主体部分。 空腔由第一面和第二面形成。 热循环装置位于空腔中。 加热器跟踪单元连接到支撑部分，连接到第一热循环器主体部分，连接到第二热循环器主体部分，并连接到热循环单元。 第一热循环器主体部分和第二热循环器主体部分一起被放置在支撑部分上以包围热循环单元和加热器跟踪单元。
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公开(公告)号：US20110022344A1
公开(公告)日：2011-01-27
申请号：US12698785
申请日：2010-02-02
Applicant: Lance F. Bentley TAMMERO , John M. Dzenitis , Benjamin J. Hindson
Inventor： Lance F. Bentley TAMMERO , John M. Dzenitis , Benjamin J. Hindson
CPC classification number: G06F19/24 , C12Q1/6813 , C12Q1/6851 , C12Q1/6888 , C12Q2537/143 , C12Q2537/165 , G01N33/53 , G01N33/569 , G01N35/00613 , G01N35/00693 , G06F19/20 , G06K9/6217 , G06K9/626 , C12Q2545/101 , C12Q2545/114
Abstract: Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.
Abstract translation: 描述了改善阵列测定性能的方法。 测定荧光强度相关参数与阴性对照值之间的相关性。 然后根据相关性来调整参数。 结果，在不改变其特异性的情况下改进了测定的灵敏度。
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公开(公告)号：US20110021368A1
公开(公告)日：2011-01-27
申请号：US12607956
申请日：2009-10-28
Applicant: Lance F.Bentley TAMMERO , John M. DZENITIS , Benjamin J. HINDSON
Inventor： Lance F.Bentley TAMMERO , John M. DZENITIS , Benjamin J. HINDSON
CPC classification number: G06F19/24 , C12Q1/6813 , C12Q1/6851 , C12Q1/6888 , C12Q2537/143 , C12Q2537/165 , G01N33/53 , G01N33/569 , G01N35/00613 , G01N35/00693 , G06F19/20 , G06K9/6217 , G06K9/626 , C12Q2545/101 , C12Q2545/114
Abstract: Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.
Abstract translation: 描述了用于确定测定中包含的特征的阈值的方法。 每个签名都可以检测目标。 该方法确定阴性样本的概率密度函数和相应的假阳性率曲线。 建立假阳性标准，并将该签名的阈值确定为假阳性率曲线与假阳性标准相交的点。 还描述了用于定量分析和解释测定结果的方法以及用于测定测定中希望的检测限的检测限的方法。
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公开(公告)号：US5279482A
公开(公告)日：1994-01-18
申请号：US892053
申请日：1992-06-05
Applicant: John M. Dzenitis , Linda W. Billica
Inventor： John M. Dzenitis , Linda W. Billica
CPC classification number: F42B12/66 , B64G1/648 , F41H13/0006
Abstract: A bola body, a bola, and methods employing such bola bodies and bolas; in one aspect a bola body having a non-spherical body member from which extend one or more fingers for entanglement with a bola line, with each other, or for latching onto part of a target body.
Abstract translation: bola体，bola和采用这种bola体和bola的方法; 在一个方面，一种具有非球形本体构件的凸起体，其从其延伸一个或多个手指以与bola线相互缠绕，或者用于锁定到目标体的一部分上。
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10.发明授权Systems and methods for separating particles and/or substances from a sample fluid 有权用于从样品流体中分离颗粒和/或物质的系统和方法公开(公告)号：US09480935B2
公开(公告)日：2016-11-01
申请号：US12024762
申请日：2008-02-01
Applicant: Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
Inventor： Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
CPC classification number: B01D17/06 , G01N1/4077 , G01N2001/4072 , G01N2015/0288
Abstract: Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chamber, the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.
Abstract translation: 用于从样品流体中分离颗粒和/或毒素的系统和方法。 根据一个实施方案的方法包括同时使样品流体和缓冲流体通过室，使得当流体通过室时在样品流体和缓冲流体之间形成流体界面，样品流体具有目标颗粒 ; 向流体施加力以促使感兴趣的颗粒通过界面进入缓冲流体; 并且从样品流体基本上分离缓冲流体。
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