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    Peptide antibody depletion and its application to mass spectrometry sample preparation
    1.
    发明授权
    Peptide antibody depletion and its application to mass spectrometry sample preparation 有权
    肽抗体耗竭及其在质谱法样品制备中的应用

    公开(公告)号：US08771969B2

    公开(公告)日：2014-07-08

    申请号：US13536047

    申请日：2012-06-28

    Applicant: Jennifer E. Van Eyk ,  David Raymond Graham ,  Rebekah Lynn Gunrdy

    Inventor： Jennifer E. Van Eyk ,  David Raymond Graham ,  Rebekah Lynn Gunrdy

    IPC: G01N31/00 ,  G01N33/53 ,  G01N33/68

    CPC classification number: G01N33/6842 ,  G01N33/6848 ,  Y10T436/25125

    Abstract: The present invention relates, e.g., to a method for pre-processing a sample for mass spectral analysis, comprising cleaving proteins in the sample to peptides and immunodepleting highly abundant and/or well-ionizing and/or proteotypic peptides from the sample. Also described are methods for identifying well-ionizing peptides for use in this and other methods; analytic (diagnostic) methods using antibodies against highly ionizable peptides from a protein target of interest; and compositions, kits and devices comprising antibodies of the invention.

    Abstract translation: 本发明涉及例如用于预处理用于质谱分析的样品的方法，包括将样品中的蛋白质切割成肽并从样品中免疫去除高度丰富和/或充分电离和/或蛋白质型肽。 还描述了用于鉴定用于该方法和其它方法的良好电离肽的方法; 使用针对来自目的蛋白靶标的高度可离子化肽的抗体的分析（诊断）方法; 以及包含本发明抗体的组合物，试剂盒和装置。

    Methods of diagnosing muscle damage
    4.
    发明授权
    Methods of diagnosing muscle damage 失效
    诊断肌肉损伤的方法

    公开(公告)号：US07618782B1

    公开(公告)日：2009-11-17

    申请号：US09419901

    申请日：1999-10-18

    Applicant: Jennifer E. Van Eyk ,  Ralf Labugger ,  Irena Neverova

    Inventor： Jennifer E. Van Eyk ,  Ralf Labugger ,  Irena Neverova

    IPC: G01N33/53 ,  G01N33/543

    CPC classification number: G01N33/6887 ,  G01N2800/10

    Abstract: A method for assessing muscle damage in a biological sample obtained from a subject is disclosed. The method involves obtaining a biological sample from a subject being assessed for muscle damage, and evaluating the sample for the presence or absence of a myofilament protein modification product. Preferably, the myofilament protein modification product is a chemical adduct of a myofilament protein. The method can also be used to assess the extent and/or type of muscle damage in a subject by studying the profile of myofilament protein modification products detected in the sample taken from the subject. The invention further provides a method for screening for an agent which modulates the level of a myofilament protein modification product an present in a biological sample or for a calcium sensitizing agent. The invention is applicable to cardiac muscle and skeletal muscle.

    Abstract translation: 公开了从受试者获得的生物样品中评估肌肉损伤的方法。 该方法包括从被评估肌肉损伤的受试者获得生物样品，以及评价样品是否存在肌丝蛋白质修饰产物。 优选地，肌丝蛋白质修饰产物是肌丝蛋白的化学加合物。 该方法还可用于通过研究从受试者取得的样品中检测到的肌丝蛋白修饰产物的轮廓来评估受试者的肌肉损伤的程度和/或类型。 本发明还提供了筛选调节生物样品或钙敏化剂中存在的肌丝蛋白修饰产物的水平的试剂的方法。 本发明适用于心肌和骨骼肌。

    Novel Phosphorylation of Cardiac Troponin I as a Monitor for Cardiac Injury
    6.
    发明申请
    Novel Phosphorylation of Cardiac Troponin I as a Monitor for Cardiac Injury 审中-公开
    心肌肌钙蛋白I的新型磷酸化作为心脏损伤监测

    公开(公告)号：US20130101606A1

    公开(公告)日：2013-04-25

    申请号：US13579797

    申请日：2011-02-17

    Applicant: Pingbo Zhang ,  Jennifer E. Van Eyk ,  Anne M. Murphy ,  Ger Stienen ,  Jolanda van der Velden ,  Viola Kooij

    Inventor： Pingbo Zhang ,  Jennifer E. Van Eyk ,  Anne M. Murphy ,  Ger Stienen ,  Jolanda van der Velden ,  Viola Kooij

    IPC: G01N33/68

    CPC classification number: G01N33/6887 ,  B82Y15/00 ,  C07K16/18 ,  C07K2317/34 ,  G01N2800/325 ,  Y10S977/774

    Abstract: This invention relates to novel phosphorylation sites in cardiac Troponin I that are associated with the onset of heart failure. The phosphorylation sites, i.e., serine 5, tyrosine 26, threonine 51, serine 166, threonine 181 and/or serine 199, can be used as biomarkers for (i) identifying subjects at risk for the development of heart failure, (ii) treating subjects having a higher than normal level of the biomarker, and (iii) monitoring therapy of a subject at risk for the development of heart failure. Also described are antibodies, reagents, and kits for carrying out a method of the present invention.

    Abstract translation: 本发明涉及与心力衰竭发作相关的心肌肌钙蛋白I中的新型磷酸化位点。 可以将磷酸化位点，即丝氨酸5，酪氨酸26，苏氨酸51，丝氨酸166，苏氨酸181和/或丝氨酸199用作生物标志物，用于（i）识别有风险发展心力衰竭的受试者，（ii）治疗 具有高于正常水平的生物标志物的受试者，以及（iii）监测患有心力衰竭发展风险的受试者的治疗。 还描述了用于实施本发明的方法的抗体，试剂和试剂盒。

    Peptide antibody depletion and its application to mass spectrometry sample preparation
    8.
    发明授权
    Peptide antibody depletion and its application to mass spectrometry sample preparation 有权
    肽抗体耗竭及其在质谱法样品制备中的应用

    公开(公告)号：US08232066B2

    公开(公告)日：2012-07-31

    申请号：US12307510

    申请日：2007-07-03

    Applicant: Jennifer E. Van Eyk ,  David Raymond Graham ,  Rebekah Lynn Gundry

    Inventor： Jennifer E. Van Eyk ,  David Raymond Graham ,  Rebekah Lynn Gundry

    IPC: G01N31/00 ,  G01N33/53

    CPC classification number: G01N33/6842 ,  G01N33/6848 ,  Y10T436/25125

    Abstract: The present invention relates, e.g., to a method for pre-processing a sample for mass spectral analysis, comprising cleaving proteins in the sample to peptides and immunodepleting highly abundant and/or well-ionizing and/or proteotypic peptides from the sample. Also described are methods for identifying well-ionizing peptides for use in this and other methods; analytic (diagnostic) methods using antibodies against highly ionizable peptides from a protein target of interest; and compositions kits and devices comprising antibodies of the invention.

    Abstract translation: 本发明涉及例如用于预处理用于质谱分析的样品的方法，包括将样品中的蛋白质切割成肽并从样品中免疫去除高度丰富和/或充分电离和/或蛋白质型肽。 还描述了用于鉴定用于该方法和其它方法的良好电离肽的方法; 使用针对来自目的蛋白靶标的高度可离子化肽的抗体的分析（诊断）方法; 以及包含本发明抗体的组合物试剂盒和装置。

    Methods and kits for separation and detection of proteins in biological samples
    10.
    发明授权
    Methods and kits for separation and detection of proteins in biological samples 失效
    用于分离和检测生物样品中蛋白质的方法和试剂盒

    公开(公告)号：US07709193B2

    公开(公告)日：2010-05-04

    申请号：US11138184

    申请日：2005-05-26

    Applicant: Jennifer E. Van Eyk ,  Jeremy A. C. Simpson

    Inventor： Jennifer E. Van Eyk ,  Jeremy A. C. Simpson

    IPC: C12Q1/68

    CPC classification number: G01N33/6803 ,  G01N33/52 ,  G01N33/6887 ,  G01N2333/4712 ,  G01N2550/00

    Abstract: Methods and kits are provided for separating a mixture of proteins in a biological sample. Methods for detecting and profiling proteins in biological samples by the separation method and kits are also provided. These methods are particularly useful in assessing damage to cells such as cardiac and skeletal muscle cells and in the early clinical diagnosis of myocardial damage by detection of myofilament proteins in serum of a subject.

    Abstract translation: 提供了用于分离生物样品中的蛋白质混合物的方法和试剂盒。 还提供了通过分离方法和试剂盒检测和分析生物样品中蛋白质的方法。 这些方法在评估对心脏和骨骼肌细胞的细胞的损伤以及通过检测受试者的血清中的肌丝蛋白质的早期心肌损伤的临床诊断中特别有用。

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