公开(公告)号：US08877511B2

公开(公告)日：2014-11-04

申请号：US12615844

申请日：2009-11-10

申请人： Jason Bedre ,  Don Chandler ,  Ben Mize

发明人： Jason Bedre ,  Don Chandler ,  Ben Mize

IPC分类号： G01N21/76 ,  G01N33/543

CPC分类号： C08J9/26 ,  C08J2325/06 ,  C09B67/006 ,  G01N33/54313 ,  H01F1/00

摘要： Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

摘要翻译： 大孔珠和制造和使用这种大孔珠的方法。 其中所述珠可区分用于多重测定中。 优选地，珠粒由两种或更多种唯一的荧光染料区分，并且至少一些珠粒是磁性响应的。 在优选形式中，一些大孔珠具有与外表面不同部分的内部孔，允许具有不同附着官能团的珠。

公开(公告)号：US20050012034A1

公开(公告)日：2005-01-20

申请号：US10885420

申请日：2004-07-06

申请人： Don Chandler

发明人： Don Chandler

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N33/58 ,  H01J5/16

CPC分类号： G01N21/645 ,  G01N2021/6421

摘要： Various computer-implemented methods and systems are provided. One computer-implemented method includes determining a ratio between output signals generated by detecting spectra for a single event in two or more detection windows. The spectra are characteristic of different materials. At least a portion of the spectra overlap in at least one of the two or more detection windows. The method also includes determining which of the different materials are associated with the ratio. One embodiment of a system includes one or more detectors configured to detect spectra for a single event in two or more detection windows. The spectra may include spectra as described above. The one or more detectors are also configured to generate output signals in response to the detected spectra. The system also includes a processor configured to determine a ratio between the output signals and to determine which of the different materials are associated with the ratio.

摘要翻译： 提供了各种计算机实现的方法和系统。 一种计算机实现的方法包括确定通过在两个或更多个检测窗口中检测单个事件的光谱而产生的输出信号之间的比率。 光谱是不同材料的特征。 至少一部分光谱在两个或更多个检测窗口中的至少一个中重叠。 该方法还包括确定哪种不同的材料与该比率相关联。 系统的一个实施例包括一个或多个检测器，其被配置为在两个或更多个检测窗口中检测单个事件的光谱。 光谱可以包括如上所述的光谱。 一个或多个检测器还被配置为响应于检测到的光谱而产生输出信号。 该系统还包括处理器，其被配置为确定输出信号之间的比率并且确定哪个不同的材料与该比率相关联。

公开(公告)号：US20100120165A1

公开(公告)日：2010-05-13

申请号：US12615844

申请日：2009-11-10

申请人： Jason Bedre ,  Don Chandler ,  Ben Mize

发明人： Jason Bedre ,  Don Chandler ,  Ben Mize

IPC分类号： G01N21/64 ,  C08J9/16

CPC分类号： C08J9/26 ,  C08J2325/06 ,  C09B67/006 ,  G01N33/54313 ,  H01F1/00

摘要： Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

摘要翻译： 大孔珠和制造和使用这种大孔珠的方法。 其中所述珠可区分用于多重测定中。 优选地，珠粒由两种或更多种唯一的荧光染料区分，并且至少一些珠粒是磁性响应的。 在优选形式中，一些大孔珠具有与外表面不同部分的内部孔，允许具有不同附着官能团的珠。

公开(公告)号：US20060159962A1

公开(公告)日：2006-07-20

申请号：US11335139

申请日：2006-01-19

申请人： Don Chandler ,  Jason Bedre

发明人： Don Chandler ,  Jason Bedre

IPC分类号： B32B15/00 ,  H01F1/00 ,  C09K11/06

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  C09K11/025 ,  C09K11/06 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6486 ,  H01F1/0018 ,  H01F1/0036 ,  H01F1/0315 ,  H01F1/063 ,  H01F1/111 ,  H01F1/36 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998 ,  Y10T428/32

摘要： Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.

公开(公告)号：US20050260676A1

公开(公告)日：2005-11-24

申请号：US11195185

申请日：2005-08-02

申请人： Don Chandler ,  Van Chandler ,  Beth Lambert ,  Janet Reber ,  Stacie Phipps

发明人： Don Chandler ,  Van Chandler ,  Beth Lambert ,  Janet Reber ,  Stacie Phipps

IPC分类号： G01N21/64 ,  C08K5/00 ,  C08L101/00 ,  C12Q1/68 ,  D06P1/00 ,  D06P1/90 ,  D06P1/92 ,  D06P5/00 ,  G01N33/533 ,  G01N33/543 ,  G01N33/551

CPC分类号： G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

摘要： An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

摘要翻译： 提供了一种制备一系列珠或微球或颗粒群的改进方法，其特征在于分布在每个群体的单个珠粒内的至少两种荧光染料的比例或比例的微小变化。 这些珠当被单个激发光源激发时能够同时发出几个荧光信号。 提供了含有多达64个不同种群的多色荧光珠的组，并且当与结合到这种珠的表面的分析试剂组合时，对于单个样品中的多种分析物的多重分析非常有用。 因此，要求保护染色聚合物颗粒，颗粒本身和使用这些颗粒的方法。

公开(公告)号：US20050164261A1

公开(公告)日：2005-07-28

申请号：US11027651

申请日：2004-12-31

申请人： Don Chandler ,  Van Chandler

发明人： Don Chandler ,  Van Chandler

IPC分类号： C12Q1/68 ,  G01N33/551

CPC分类号： G01N33/551

摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. A secondary reagent, such as a metal or magnetic particle, is added to the beadset to assist in the analysis.

摘要翻译： 用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。 流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。 将诸如金属或磁性颗粒的次级试剂加入珠粒以帮助分析。

公开(公告)号：US20050277197A1

公开(公告)日：2005-12-15

申请号：US11173331

申请日：2005-06-30

申请人： Mark Chandler ,  Don Chandler ,  Jason Bedre

发明人： Mark Chandler ,  Don Chandler ,  Jason Bedre

IPC分类号： C12Q1/68 ,  G01N15/14 ,  G01N33/00 ,  G01N33/58

CPC分类号： G01N33/587 ,  C12Q1/6816 ,  G01N15/14 ,  G01N33/582 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/823 ,  Y10S977/707 ,  Y10S977/773 ,  Y10S977/904 ,  Y10S977/92 ,  Y10T436/10 ,  Y10T436/109163 ,  Y10T436/13 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2537/143 ,  C12Q2565/626

摘要： This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.

摘要翻译： 本发明提供了一种新颖的荧光颗粒，其包括在其表面上具有多个较小的聚合物颗粒或纳米颗粒的芯或载体颗粒，其被不同的荧光染料染色。 当被光源激发时，它们能够同时发出多个荧光发射，这对于样品中多个分析物的多重分析是有用的。 涉及其表面上携带有荧光纳米颗粒的偶联复合颗粒，制备这种聚合物颗粒的方法以及使用这种颗粒的各种用途和方法。

公开(公告)号：US20050202469A1

公开(公告)日：2005-09-15

申请号：US11027652

申请日：2004-12-31

申请人： Don Chandler ,  Van Chandler

发明人： Don Chandler ,  Van Chandler

IPC分类号： C12Q1/68 ,  G01N33/553

CPC分类号： G01N33/54313 ,  C12Q1/6834 ,  G01N33/537 ,  Y10S435/973 ,  C12Q2563/149

摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. A secondary reagent, such as a metal or magnetic particle, is added to the beadset to assist in the analysis. Detection techniques, such as such as light scatter, Rayleigh scatter, Raman scatter, surface plasmon resonance, magnetic induction, or magnetoresistance are used to detect the particle labels.

摘要翻译： 用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。 流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。 将诸如金属或磁性颗粒的次级试剂加入珠粒以帮助分析。 使用诸如光散射，瑞利散射，拉曼散射，表面等离子体共振，磁感应或磁阻等检测技术来检测颗粒标签。