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公开(公告)号：US08914241B2
公开(公告)日：2014-12-16
申请号：US12878687
申请日：2010-09-09
CPC分类号： C12Q1/6874 , C12Q1/686 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2565/501 , C12Q2537/143
摘要： A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
摘要翻译： 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。
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公开(公告)号：US20110143965A1
公开(公告)日：2011-06-16
申请号：US13023406
申请日：2011-02-08
申请人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
发明人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00295 , B01J2219/00308 , B01J2219/00315 , B01J2219/00326 , B01J2219/00328 , B01J2219/00369 , B01J2219/00389 , B01J2219/00412 , B01J2219/00421 , B01J2219/00423 , B01J2219/00454 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14 , G01N35/025 , G01N35/028 , G01N35/1067 , G01N2035/00504 , G01N2035/1037 , G01N2035/1069 , Y10T436/111666 , Y10T436/12 , Y10T436/2575
摘要： An apparatus for high-throughput combinatorial synthesis of organic molecules including a reaction vessel for containing a combinatorial chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis or rotation. A method of combinatorial synthesis of organic molecules is also disclosed.
摘要翻译： 一种用于高通量组合合成有机分子的装置，包括用于包含组合化学合成反应的反应容器，用于分配液体的液体分配器，液体抽吸器和调节机构。 反应容器包括允许液体进入容器内部的入口孔和用于从容器吸出液体的出口孔。 液体分配器通过入口孔分配液体。 液体吸入器通过出口孔吸出液体，并且包括用于承载容器并绕容器围绕旋转轴线运动的转子。 转子通常在水平面定向，并且包括调节机构，用于响应于通过围绕轴线或旋转绕过容器而产生的离心力来调节容器相对于水平面的角度。 还公开了组合合成有机分子的方法。
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公开(公告)号：US06663832B2
公开(公告)日：2003-12-16
申请号：US09738473
申请日：2000-12-13
申请人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
发明人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
IPC分类号： G01N2100
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00295 , B01J2219/00308 , B01J2219/00315 , B01J2219/00326 , B01J2219/00328 , B01J2219/00369 , B01J2219/00389 , B01J2219/00412 , B01J2219/00421 , B01J2219/00423 , B01J2219/00454 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N35/025 , G01N35/028 , G01N35/1067 , G01N2035/00504 , G01N2035/1037 , G01N2035/1069 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/2575
摘要： An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.
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公开(公告)号：US08725425B2
公开(公告)日：2014-05-13
申请号：US12020739
申请日：2008-01-28
申请人： David L. Heiner , Robert C. Kain
发明人： David L. Heiner , Robert C. Kain
CPC分类号： C12Q1/6874 , C12Q1/686 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2565/501 , C12Q2537/143
摘要： A technique is disclosed for performing sequencing of polymers such as DNA and RNA. A sample containing multiple sites to be sequenced is cyclically subjected to attachment of nucleotides, and imaging. A digital mask corresponding to sites of interest in the sample array is generated and image data for the sites is processed differently from image data not corresponding to sites of interest. The latter may be discarded during the sequencing operation. The use of the mask improves computational efficiency and reduces memory allocated for the image data during sequencing.
摘要翻译： 公开了一种用于进行聚合物如DNA和RNA的测序的技术。 含有待测序的多个位点的样品循环地进行核苷酸的附着和成像。 产生对应于样本阵列中的感兴趣位点的数字掩码，并且处理不同于不对应于感兴趣的位点的图像数据的站点的图像数据。 后者可能在排序操作期间丢弃。 掩码的使用提高了计算效率，并减少了排序期间为图像数据分配的内存。
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公开(公告)号：US08315817B2
公开(公告)日：2012-11-20
申请号：US12492073
申请日：2009-06-25
CPC分类号： C12Q1/6874 , C12Q1/686 , C12Q1/6869 , C12Q2565/501 , C12Q2537/143
摘要： A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
摘要翻译： 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。
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6.发明申请公开(公告)号：US20120129704A1
公开(公告)日：2012-05-24
申请号：US13260205
申请日：2010-04-02
申请人： Kevin Gunderson , Michal Lebl , David L. Heiner
发明人： Kevin Gunderson , Michal Lebl , David L. Heiner
CPC分类号： C12N15/10 , C12N15/101 , Y10T436/143333
摘要： Methods of generating nucleic acid fragments of substantially uniform length from sample nucleic acids comprising linearly stretching the sample nucleic acids over a substrate having a plurality of cleavage regions separated by relatively consistent distances, cleaving the linearly stretched sample nucleic acids at the cleavage regions, and collecting the resulting nucleic acid fragments. The method may further include collecting and concentrating the resultant nucleic acid fragments of substantially uniform length.
摘要翻译： 从样品核酸产生具有基本上均匀长度的核酸片段的方法，包括在具有通过相对一致距离分离的多个切割区域的底物上线性拉伸样品核酸，在切割区域切割线性拉伸的样品核酸，并收集 得到的核酸片段。 该方法还可以包括收集和浓缩所得到的基本上均匀长度的核酸片段。
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公开(公告)号：US20110136696A1
公开(公告)日：2011-06-09
申请号：US13029071
申请日：2011-02-16
申请人： David L. Heiner , Aaron C. Jones , Steven P. Fambro , Mark J. Nibbe , Steve R. Burgett , Brett M. Ellman , Michal Lebl
发明人： David L. Heiner , Aaron C. Jones , Steven P. Fambro , Mark J. Nibbe , Steve R. Burgett , Brett M. Ellman , Michal Lebl
IPC分类号： C40B50/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00283 , B01J2219/00315 , B01J2219/00326 , B01J2219/0036 , B01J2219/00423 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00612 , B01J2219/00659 , B01J2219/00675 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , C40B60/14 , Y10T436/115831 , Y10T436/12
摘要： Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.
摘要翻译： 描述了用于合成聚合物分子如寡核苷酸和多肽的系统和方法。 该系统能够通过提供反应位点阵列和用于进行合成操作的站阵列来连续合成分子。 前一阵列中的反应位点可以相对于彼此以固定的顺序和固定的间隔放置。 类似地，站可以以固定的顺序相对于彼此以固定的间隔放置。 两个阵列可以相对于彼此移动，使得站在每个反应位置进行反应方案的期望步骤。 站的相对位置和相对运动的时间表可以与反应方案中的反应步骤的顺序和持续时间相关联，使得一旦反应位点完成了与整个站阵列相互作用的循环，则反应方案为 完成。
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公开(公告)号：US20110072914A1
公开(公告)日：2011-03-31
申请号：US12031054
申请日：2008-02-14
申请人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
发明人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
CPC分类号： F04B19/006 , B01L3/502715 , B01L3/50273 , B01L7/00 , B01L2300/0816 , B01L2300/0877 , B01L2400/0418 , G01N35/00613 , G01N35/1095 , Y10T436/2575
摘要： A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
摘要翻译： 提供了一种用于微流体检测系统的流通池。 流动池包括具有通道的流动池主体，该通道被配置为将溶液输送通过流动池主体。 流动池还包括底面和顶面。 底表面被构造成由检测系统可移除地保持，并且顶表面是透明的并且允许光通过。 流动池体还包括与通道流体连通的流体入口和出口。 在流动池主体中还设有泵腔。 泵腔与通道的端部和流体入口和出口之一流体连通并且介于其间。 电渗（EO）泵保持在泵腔中。 EO泵通过EO泵和流体入口和出口之间的通道引起溶液的流动。
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公开(公告)号：US07835871B2
公开(公告)日：2010-11-16
申请号：US12020721
申请日：2008-01-28
CPC分类号： C12Q1/6874 , C12Q1/686 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2565/501 , C12Q2537/143
摘要： A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
摘要翻译： 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。
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10.发明申请NUCLEIC ACID SEQUENCING SYSTEM AND METHOD USING A SUBSET OF SITES OF A SUBSTRATE 有权核酸序列测序系统和使用基片的位点的方法公开(公告)号：US20100138162A1
公开(公告)日：2010-06-03
申请号：US12492078
申请日：2009-06-25
CPC分类号： C12Q1/6874 , C12Q1/686 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2565/501 , C12Q2537/143
摘要： A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
摘要翻译： 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。
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