公开(公告)号：US09766239B2

公开(公告)日：2017-09-19

申请号：US14776434

申请日：2014-03-12

Applicant: Nidhi Gupta ,  Chris Diehnelt ,  Charles Arntzen ,  Stephen Johnston

Inventor： Nidhi Gupta ,  Chris Diehnelt ,  Charles Arntzen ,  Stephen Johnston

IPC: C07K16/08 ,  G01N33/569 ,  C07K14/00

CPC classification number: G01N33/56983 ,  C07K14/001 ,  C07K16/08 ,  C07K2318/20 ,  G01N2333/08 ,  G01N2469/10 ,  G01N2800/26

Abstract: Synbodies specific for Norovirus and coupled with a substrate provide Norovirus binding and detection platforms (FIG. 1). A Norovirus capturing platform, comprising one or more synbodies selected from the group consisting of synbodies 6-6, 92-92, 93-93, and 94-94 coupled to a substrate, has been found to found to bind with either GII.4 Minerva or both GII.4 Minerva and GII.4 Sydney# strains of norovirus.

公开(公告)号：US20060288442A1

公开(公告)日：2006-12-21

申请号：US11507106

申请日：2006-08-21

Applicant: Charles Arntzen ,  Peter Kipp ,  Ramesh Kumar ,  Gregory May

Inventor： Charles Arntzen ,  Peter Kipp ,  Ramesh Kumar ,  Gregory May

IPC: A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8213 ,  C07K14/43595 ,  C12N15/102 ,  C12N15/8207 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8274 ,  C12N15/8289

Abstract: The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electroporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated form the altered cells. In specific embodiments the invention concerns the alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or homolog of etr-1, and plants having isolated point mutations in such genes.

Abstract translation: 本发明涉及使用双链寡核苷酸约25至30个碱基对来引入植物细胞中的位点特异性遗传改变。 寡核苷酸可以通过机械（生物弹道）系统或通过电穿孔植物原生质体递送。 此后可以从改变的细胞产生具有遗传改变的植物。 在具体实施方案中，本发明涉及编码酸转化酶，UDP-葡萄糖焦磷酸化酶，多酚氧化酶，O-甲基转移酶，肉桂醇脱氢酶，ACC合酶和ACC氧化酶或etr-1或etr-1的同源物的基因的改变，以及 在这些基因中具有孤立点突变的植物。

公开(公告)号：US20050232949A1

公开(公告)日：2005-10-20

申请号：US11127965

申请日：2005-05-12

Applicant: Charles Arntzen ,  Dominic Lam

Inventor： Charles Arntzen ,  Dominic Lam

IPC: A01H1/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/205 ,  A61K39/29 ,  C07K14/02 ,  C12N15/82

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/5258 ,  A61K2039/542 ,  C07K2319/00 ,  C12N15/8242 ,  C12N15/8258 ,  C12N2730/10122 ,  C12N2730/10134

Abstract: The anti-viral vaccine of the present invention is produced in transgenic plants and then administered through standard vaccine introduction method or through the consumption of the edible portion of those plants. A DNA sequence encoding for the expression of a surface antigen of a viral pathogen is isolated and ligated to a promoter which can regulate the production of the surface antigen in a transgenic plant. This gene is then transferred to plant cells using a procedure that results in its integration into the plant genome, such as through the use of an Agrobacterium tumenfaciens plasmid vector system. Preferably, the foreign gene is expressed in an portion of the plant that is edible by humans or animals. In a preferred procedure, the vaccine is administered through the consumption of the edible plant as food, preferably in the form of a fruit or vegetable juice which can be taken orally.

Abstract translation: 本发明的抗病毒疫苗在转基因植物中产生，然后通过标准疫苗引入方法或通过消耗这些植物的可食用部分来施用。 分离编码病毒病原体的表面抗原表达的DNA序列，并与可以调节转基因植物中表面抗原产生的启动子连接。 然后使用导致其整合到植物基因组中的程序，例如通过使用农杆菌质粒载体系统将该基因转移到植物细胞。 优选地，外源基因在植物的可被人或动物食用的部分中表达。 在优选的方法中，通过食用可食用植物作为食物来施用疫苗，优选以可以口服的水果或蔬菜汁的形式。

公开(公告)号：US10125373B2

公开(公告)日：2018-11-13

申请号：US14762128

申请日：2014-01-22

Applicant: THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY ,  Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andrew Diamos

Inventor： Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andrew Diamos

IPC: C12N15/82 ,  C07K16/28 ,  C12N7/00

Abstract: A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.

公开(公告)号：US20060013831A1

公开(公告)日：2006-01-19

申请号：US10506796

申请日：2003-03-06

Applicant: Tsafrir Mor ,  Nobuyuki Matoba ,  Charles Arntzen

Inventor： Tsafrir Mor ,  Nobuyuki Matoba ,  Charles Arntzen

IPC: C12Q1/68 ,  G01N33/53 ,  A61K39/12

CPC classification number: C07K14/005 ,  A61K39/39 ,  A61K2039/541 ,  A61K2039/55516 ,  A61K2039/6037 ,  A61K2039/6075 ,  C12N2740/16122

Abstract: A composition and method for enhancing immune response in a living organism is disclosed. In particular, the present disclosure provides an adjuvant peptide for use in raising an immune response to an antigen. The adjuvant peptide is selected from a group of peptides with an HIV-related sequence. Additionally, the adjuvant peptide can comprise a fusion-protein that acts as a mucosal adjuvant. The adjuvant peptide can be transformed into one or more living cells, such that the mucosal adjuvant can be produced in living cells and then administered by systemic, mucosal or epidermal delivery.

Abstract translation: 公开了一种增强活体内免疫应答的组合物和方法。 特别地，本公开提供了用于提高对抗原的免疫应答的佐剂肽。 佐剂肽选自具有HIV相关序列的肽组。 另外，佐剂肽可以包含充当粘膜佐剂的融合蛋白。 辅助肽可以转化成一个或多个活细胞，使得粘膜佐剂可以在活细胞中产生，然后通过全身，粘膜或表皮递送进行施用。

公开(公告)号：US20050114924A1

公开(公告)日：2005-05-26

申请号：US10958324

申请日：2004-10-06

Applicant: Hortense Dodo ,  Charles Arntzen ,  Olga Viquez ,  Koffi Konan

Inventor： Hortense Dodo ,  Charles Arntzen ,  Olga Viquez ,  Koffi Konan

IPC: C07K14/415 ,  C12N15/82 ,  A01H1/00

CPC classification number: C12N15/8251 ,  C07K14/415 ,  C12N15/8242

Abstract: An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position−72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

Abstract translation: 通过重组方法产生无过敏原的转基因花生种子。 每个变应原基因或其片段的多个拷贝转化花生植物以抑制基因表达和变应原蛋白质的产生。 或者，用作为反义片段，有义片段或反义片段和有义片段的组合引入花生基因组的花生过敏原反义基因转化花生植物。 花生转基因由35S启动子或Ara h2基因的启动子控制，以产生用于抑制花生植物中过敏原蛋白质产生的反义RNA，有义RNA和双链RNA。 分离和测序过敏原Ara h2的全长基因组克隆。 ORF为622个核苷酸长。 预测编码的蛋白质是207个氨基酸长，并且包含21个残基的推定转运肽。 在951位点识别出一种多聚增生信号。观察到另外六个终止密码子。 揭示了一个启动子区域，其中包含位于-72位置的假定TATA盒。 在Ara h2，h6和h7之间以及Ara h3和h4之间以及Ara h1P41B和Ara h1P17之间鉴定了同源区域。 同源区域将用于筛选花生基因组文库，以分离所有花生过敏原基因并降低多个花生过敏原基因的沉默。

公开(公告)号：US20150368660A1

公开(公告)日：2015-12-24

申请号：US14762128

申请日：2014-01-22

Applicant: Hugh MASON ,  Charles ARNTZEN ,  Sun Hee ROSENTHAL ,  Sean WINKLE ,  Andy DIAMOS ,  THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andy Diamos

IPC: C12N15/82 ,  C12N7/00 ,  C07K16/28

CPC classification number: C12N15/8258 ,  C07K16/2887 ,  C07K2317/13 ,  C07K2317/14 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8218 ,  C12N2750/12043

Abstract: A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.

Abstract translation: 描述了含有两个或更多个非竞争性复制子的单个载体或多个单独的载体，用于在烟草本生叶中短暂表达利妥昔单抗的重链和轻链。 还描述了将这些亚基蛋白正确组装到功能性低聚结构中以优化表达。 该系统通过消除对非竞争病毒的需要，推进植物瞬时表达技术，从而增强了用于在植物细胞中生产利妥昔单抗的多复制子单载体系统的实际商业应用。

公开(公告)号：US20060073222A1

公开(公告)日：2006-04-06

申请号：US11295189

申请日：2005-12-06

Applicant: Charles Arntzen ,  Mary Blake ,  Jordan Gutterman

Inventor： Charles Arntzen ,  Mary Blake ,  Jordan Gutterman

IPC: A61K36/48 ,  A61K31/704

CPC classification number: A61K41/0038 ,  A01H4/005 ,  A23L33/105 ,  A61K31/70 ,  A61K31/704 ,  A61K36/48 ,  C12N5/04 ,  Y10S514/821 ,  Y10S514/886 ,  Y10S514/887 ,  A61K2300/00

Abstract: The invention provides novel saponin mixtures and compounds which are isolated from the species Acacia victoriae and methods for their use. These compounds may contain a triterpene moiety, such as acacic or oleanolic acid, to which oligosaccharides and monoterpenoid moieties are attached. The mixtures and compounds have properties related to the regulation of apoptosis and cytotoxicity of cells and exhibit potent anti-tumor effects against a variety of tumor cells.

公开(公告)号：US20050155113A1

公开(公告)日：2005-07-14

申请号：US10895791

申请日：2004-07-21

Applicant: William Hamilton ,  Koen Hellendoorn ,  Timothy Jones ,  Dwayne Kirk ,  Hugh Mason ,  Xiuren Zhang ,  Charles Arntzen

Inventor： William Hamilton ,  Koen Hellendoorn ,  Timothy Jones ,  Dwayne Kirk ,  Hugh Mason ,  Xiuren Zhang ,  Charles Arntzen

IPC: A01H1/00 ,  A61K20060101 ,  A61K39/12 ,  C12N7/00 ,  C12N15/82 ,  C12Q1/70

CPC classification number: C12N15/8258 ,  A61K2039/5258 ,  A61K2039/542 ,  C07K14/005 ,  C12N2770/16022

Abstract: The present invention relates to a synthetic plant-optimized nucleic acid molecule having a Norwalk virus capsid protein coding nucleotide sequence, and nucleic acid constructs, host cells, expression systems, and plants having the plant-optimized Norwalk virus nucleic acid molecule. The present invention also relates to a method of producing Norwalk virus capsid protein virus-like particles in a transgenic plant or transgenic plant seed transformed with a plant-optimized nucleic acid molecule encoding Norwalk virus capsid protein. The plant or a component thereof can be administered to a subject under conditions effective to immunize the subject against disease resulting from infection by a Norovirus, including Norwalk virus. An oral vaccine for immunization of a subject against Norwalk virus infection is also disclosed.

Abstract translation: 本发明涉及具有Norwalk病毒衣壳蛋白编码核苷酸序列的合成植物优化核酸分子，以及具有植物优化诺瓦克病毒核酸分子的核酸构建体，宿主细胞，表达系统和植物。 本发明还涉及在用植物优化的编码诺瓦克病毒衣壳蛋白的核酸分子转化的转基因植物或转基因植物种子中产生诺瓦克病毒衣壳蛋白病毒样颗粒的方法。 植物或其组分可以在有效免疫受试者的病症下施用于受诺如病毒（包括诺瓦克病毒）感染引起的疾病的病症。 还公开了用于免疫Norwalk病毒感染的受试者的口服疫苗。

公开(公告)号：US06392121B1

公开(公告)日：2002-05-21

申请号：US09414276

申请日：1999-10-07

Applicant: Hugh S. Mason ,  Kenneth E. Palmer ,  Kathleen L. Hefferon ,  Tsafrir S. Mor ,  Charles Arntzen

Inventor： Hugh S. Mason ,  Kenneth E. Palmer ,  Kathleen L. Hefferon ,  Tsafrir S. Mor ,  Charles Arntzen

IPC: C12N504

CPC classification number: C12N15/8216 ,  C12N15/8203 ,  Y10S977/804

Abstract: A gene amplification system based on plant viral genetic elements dramatically increases foreign protein production in plants. A safer and more economical production system for vaccines and antibodies in recombinant plants grown using agricultural practice is described. The high-level expression system uses the replicative process of a plant mastrevirus, exemplified by bean yellow dwarf virus (BeYDV). The expression system is preferably inducible to avoid interference with plant growth and development. Developmental cues, such as fruit ripening, are employed to trigger expression of the foreign protein using a tissue-specific promoter. A single, stably integrated expression cassette for foreign protein is replicated extrachromosomally in ripening fruit, forming hundreds of transcriptionally competent copies. Preferred plant hosts include tomato as a model system and soybean for production of large quantities of protein at high total protein levels.

Abstract translation: 基于植物病毒遗传元件的基因扩增系统大大增加了植物中的外来蛋白质产量。 描述了使用农业实践生长的重组植物中更安全和更经济的疫苗和抗体生产系统。 高水平的表达系统使用植物重症病毒的复制过程，以豆黄矮病毒（BeYDV）为例。 表达系统优选可诱导以避免干扰植物生长和发育。 使用诸如果实成熟的发育提示来使用组织特异性启动子来引发外源蛋白的表达。 用于外来蛋白质的单一稳定整合的表达盒在成熟果实中被染色体外复制，形成数百个可转录的拷贝。 优选的植物宿主包括番茄作为模型系统，大豆用于生产高蛋白总量的大量蛋白质。