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    Method of identifying a nucleic acid sequence in a plant
    3.
    发明申请
    Method of identifying a nucleic acid sequence in a plant 审中-公开
    鉴定植物中核酸序列的方法

    公开(公告)号：US20030027183A1

    公开(公告)日：2003-02-06

    申请号：US10120630

    申请日：2002-04-10

    发明人： Monto H. Kumagai ,  Guy R. della-Cioppa ,  Robert L. Erwin ,  David R. McGee

    IPC分类号： C12Q001/68 ,  A01H001/00

    CPC分类号： C12Q1/68 ,  A01H1/04 ,  A01H5/12 ,  C07K14/415 ,  C12N15/1034 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/825 ,  C12N15/8257 ,  C12N15/8261 ,  Y02A40/146 ,  Y10S977/804

    摘要： The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

    摘要翻译： 本发明提供了一种编辑植物功能基因谱的方法，改变植物的表型或生物化学的方法，确定植物的表型或生物化学变化的方法以及确定性状存在的方法 在植物中。 所述方法包括将植物的核酸序列瞬时表达到宿主植物中以影响宿主植物的表型或生物化学变化。 已经开发了病毒载体功能基因组筛选，以通过在反义机制中系统敲除内源基因表达来鉴定转染植物中的核苷酸序列。 一旦通过宿主植物中的表型或生物化学变化鉴定植物中性状的存在，则对cDNA克隆中的核酸插入物或导致变化的载体进行测序。 本发明举例说明在一种植物中编码GTP结合蛋白的基因可以使不同植物中的内源基因表达沉默。

    Viral amplification of recombinant messenger RNA in transgenic plants
    4.
    发明授权
    Viral amplification of recombinant messenger RNA in transgenic plants 失效

    公开(公告)号：US06462255B1

    公开(公告)日：2002-10-08

    申请号：US09414916

    申请日：1999-10-08

    申请人： Thomas H. Turpen

    发明人： Thomas H. Turpen

    IPC分类号： C12N1583

    CPC分类号： C07K14/005 ,  C12N15/8203 ,  C12N15/8216 ,  C12N2770/00022 ,  Y10S47/01 ,  Y10S977/804 ,  Y10S977/916 ,  Y10S977/92

    摘要： A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

    Viral amplification of recombinant messenger RNA in transgenic plants
    5.
    发明申请
    Viral amplification of recombinant messenger RNA in transgenic plants 审中-公开

    公开(公告)号：US20020144308A1

    公开(公告)日：2002-10-03

    申请号：US09930342

    申请日：2001-08-14

    发明人： Thomas H. Turpen

    IPC分类号： A01H001/00 ,  C12N007/00 ,  C12N015/86

    CPC分类号： C07K14/005 ,  C12N15/8203 ,  C12N15/8216 ,  C12N2770/00022 ,  Y10S47/01 ,  Y10S977/804 ,  Y10S977/916 ,  Y10S977/92

    摘要： A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

    Compositions and methods for administering Borrelia DNA
    6.
    发明授权
    Compositions and methods for administering Borrelia DNA 失效
    组合物和施用Borrelia DNA的方法

    公开(公告)号：US06451769B1

    公开(公告)日：2002-09-17

    申请号：US09173053

    申请日：1998-10-15

    申请人： Robert C. Huebner ,  Jon A. Norman ,  Xiaowu Liang ,  Kristin R. Carner ,  Alan G. Barbour ,  Catherine J. Luke

    发明人： Robert C. Huebner ,  Jon A. Norman ,  Xiaowu Liang ,  Kristin R. Carner ,  Alan G. Barbour ,  Catherine J. Luke

    IPC分类号： A61K3170

    CPC分类号： C07K14/20 ,  A61K39/00 ,  C12N15/85 ,  Y02A50/401 ,  Y10S977/804

    摘要： Disclosed is a vaccine against Lyme Disease or its causative agent Borrelia burgdorferi (sensu stricto or sensu lato) containing a plasmid a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding Borrelia OspA or OspB, and a DNA encoding a terminator. Disclosed too is an immunogenic composition against Lyme Disease or its causative agent Borrelia burgdorferi (sensu stricto or sensu lato) containing a plasmid comprising a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding a Borrelia OspC, and a DNA encoding a terminator. And, methods for making and using such vaccines and the immunogenic composition are also disclosed.

    摘要翻译： 公开了一种针对莱姆病的疫苗或其致病剂博氏疏螺旋体（sensu stricto或sensu lato），其含有编码用于在哺乳动物细胞中驱动表达的启动子的DNA质粒，编码促进原核蛋白分泌/释放的前导肽的DNA 来自哺乳动物细胞的序列，编码Borrelia OspA或OspB的DNA，以及编码终止子的DNA。 还公开了针对莱姆病或其致病因子伯氏疏螺旋体（sensu stricto或sensu lato）的免疫原性组合物，其包含质粒，其包含编码用于在哺乳动物细胞中驱动表达的启动子的DNA，编码促进分泌/释放的前导肽的DNA 来自哺乳动物细胞的原核蛋白质序列，编码疏螺旋体OspC的DNA和编码终止子的DNA。 并且还公开了制备和使用这些疫苗的方法和免疫原性组合物。

    ATTENUATED MICROORGANISM STRAINS EXPRESSING HPV PROTEINS
    8.
    发明申请
    ATTENUATED MICROORGANISM STRAINS EXPRESSING HPV PROTEINS 失效
    表达HPV蛋白的衰老微生物菌株

    公开(公告)号：US20020025328A1

    公开(公告)日：2002-02-28

    申请号：US09288861

    申请日：1999-04-09

    发明人： DENISE NARDELLI HAEFLIGER ,  JEAN-PIERRE KRAEHENBUHL

    IPC分类号： C12Q001/70 ,  G01N033/53 ,  C07H021/04 ,  A01N063/00 ,  C12N015/74 ,  C12N015/70 ,  A61K039/112 ,  A61K039/02

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/51 ,  C07K2319/00 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2710/20023 ,  Y02A50/476 ,  Y02A50/484 ,  Y10S977/804 ,  Y10S977/904 ,  Y10S977/917

    摘要： This application relates to the use of attenuated prokaryotic microorganism strains (such as Salmonella ) expressing nucleic acid encoding HPV proteins as vaccines against HPV infection and the associated increased risk of cancer. In particular, the work shows that it is possible to assemble VLPs in a prokaryotic organism and that nasal immunization of mice with the strains HPV-specific conformationally dependent and neutralizing antibodies in serum and genital secretions. The experiments described herein show that it is also possible to assemble chimeric VLPs of an HPV including a fusion partner and that tumor protection can be induced.

    摘要翻译： 本申请涉及使用表达编码HPV蛋白的核酸的减毒原核生物微生物菌株（如沙门氏菌）作为HPV感染疫苗和相关的增加的癌症风险。 特别是，这项工作表明，有可能在原核生物体中组装VLP，并且可以在血清和生殖道分泌物中对具有HPV特异性构象依赖性和中和抗体的菌株进行鼻腔免疫。 本文所述的实验表明，还可以组装包含融合配偶体的HPV的嵌合VLP并且可以诱导肿瘤保护。

    Methods of utilizing the TT virus
    9.
    发明申请
    Methods of utilizing the TT virus 审中-公开
    TT病毒的使用方法

    公开(公告)号：US20010041331A1

    公开(公告)日：2001-11-15

    申请号：US09815656

    申请日：2001-03-23

    发明人： Thomas P. Leary ,  James C. Erker ,  Michelle L. Chalmers ,  John N. Simons ,  Larry G. Birkenmeyer ,  A. Scott Muerhoff ,  Tami J. Pilot-Matias ,  Suresh M. Desai ,  Isa K. Mushahwar

    IPC分类号： C12Q001/70 ,  C12Q001/68 ,  C12N015/40

    CPC分类号： C12Q1/701 ,  Y10S977/804

    摘要： The present invention relates to nucleic oligomer primers or probes useful for detection of TTV in test samples. Also provided are assays which utilize these primers and probes, as well as test kits which contain these oligomer primers and/or. In addition, the present invention encompasses the use of TTV nucleotide sequences as nucleic acid vectors and as markers for determining transmission between individuals as well as the route thereof. Additionally, the present invention invention encompasses a method of detecting TTV infection prior to xenotransplantation of a tissue or organ.

    摘要翻译： 本发明涉及用于在测试样品中检测TTV的核酸低聚物引物或探针。 还提供了利用这些引物和探针的测定法，以及含有这些低聚物引物和/或的这些试剂盒。 此外，本发明包括TTV核苷酸序列作为核酸载体的用途以及用于确定个体间传播的标记及其途径。 此外，本发明包括在组织或器官的异种移植之前检测TTV感染的方法。