公开(公告)号：USRE44962E1

公开(公告)日：2014-06-24

申请号：US14017108

申请日：2013-09-03

Applicant: Bayer Cropscience N.V. ,  Biogen Idec MA, Inc.

Inventor： Jan Leemans ,  Johan Botterman ,  Marc De Block ,  Charles Thompson ,  Rao Mouva

IPC: C12N15/52 ,  C12N15/31

CPC classification number: A01H1/00 ,  C07K2319/00 ,  C12N9/1029 ,  C12N15/63 ,  C12N15/70 ,  C12N15/76 ,  C12N15/8277 ,  Y10S47/01

Abstract: The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products.It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

Abstract translation: 本发明涉及一种含有确定基因的DNA片段，其表达抑制了Bialaphos及相关产品的抗生素和除草效果。 它还涉及含有这种DNA片段的重组载体，其使得能够将该保护性基因导入细胞和植物细胞中。

公开(公告)号：US06852846B2

公开(公告)日：2005-02-08

申请号：US09930329

申请日：2001-08-14

Applicant: Thomas H. Turpen

Inventor： Thomas H. Turpen

IPC: A01H5/00 ,  C07K14/08 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/40 ,  C12N15/82 ,  C12N15/83 ,  C12P21/02 ,  C12R1/92 ,  C07H21/02 ,  C12N5/14 ,  C12N7/10

CPC classification number: C07K14/005 ,  C12N15/8203 ,  C12N15/8216 ,  C12N2770/00022 ,  Y10S47/01 ,  Y10S977/804 ,  Y10S977/916 ,  Y10S977/92

Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus, the recombinant transcripts are replicated producing high levels of foreign gene RNA.

Abstract translation: 提供了一种在植物中过表达基因的新方法。 该方法基于植物正链RNA病毒的RNA扩增特性。 构建含有植物启动子，病毒复制起点，病毒运动蛋白基因以及病毒亚基因组启动子控制下的一个或多个外源基因的嵌合多顺反子基因。 含有一种或多种这些重组RNA转录物的植物用辅助病毒接种。 在辅助病毒的存在下，复制重组转录物产生高水平的外源基因RNA。

公开(公告)号：US20020104123A1

公开(公告)日：2002-08-01

申请号：US09930329

申请日：2001-08-14

Inventor： Thomas H. Turpen

IPC: A01H005/00 ,  C12N015/86

CPC classification number: C07K14/005 ,  C12N15/8203 ,  C12N15/8216 ,  C12N2770/00022 ,  Y10S47/01 ,  Y10S977/804 ,  Y10S977/916 ,  Y10S977/92

Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

Abstract translation: 提供了一种在植物中过表达基因的新方法。 该方法基于植物正链RNA病毒的RNA扩增特性。 构建含有植物启动子，病毒复制起点，病毒运动蛋白基因以及病毒亚基因组启动子控制下的一个或多个外源基因的嵌合多顺反子基因。 含有一种或多种这些重组RNA转录物的植物用辅助病毒接种。 在辅助病毒的存在下，复制重组转录物产生高水平的外源基因RNA。 提供了通过具有辅助病毒的复制子RNA扩增和源自烟草花叶病毒组的运动蛋白基因在烟草植物中高水平表达氯霉素乙酰转移酶的序列。

公开(公告)号：US20010020300A1

公开(公告)日：2001-09-06

申请号：US09138873

申请日：1998-08-24

Inventor： JURGEN LOGEMANN ,  GUIDO JACH ,  BIRGIT GORNHARDT ,  JOHN MUNDY ,  JEFF SCHELL ,  PETER ECKES ,  ILAN CHET

IPC: A61K038/47

CPC classification number: C12Y302/01014 ,  C07K14/37 ,  C07K14/415 ,  C12N9/2402 ,  C12N9/2442 ,  C12N15/8282 ,  Y10S47/01

Abstract: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).

Abstract translation: 转基因病原体抗性生物体，其基因组含有至少两种不同基因，在活性启动子控制下具有病原体抑制作用。 该生物体的特征在于协同的病原体抑制作用。 当基因编码基因产物几丁质酶（ChiS，ChiG），葡聚糖酶（GluG），蛋白质合成抑制剂（PSI）和抗真菌蛋白质（AFP））时，这种作用是显而易见的。

公开(公告)号：US06271438B1

公开(公告)日：2001-08-07

申请号：US09138873

申请日：1998-08-24

Applicant: Jürgen Logemann ,  Guido Jach ,  Birgit Görnhardt ,  John Mundy ,  Jeff Schell ,  Peter Eckes ,  Ilan Chet

Inventor： Jürgen Logemann ,  Guido Jach ,  Birgit Görnhardt ,  John Mundy ,  Jeff Schell ,  Peter Eckes ,  Ilan Chet

IPC: A01H106

CPC classification number: C12Y302/01014 ,  C07K14/37 ,  C07K14/415 ,  C12N9/2402 ,  C12N9/2442 ,  C12N15/8282 ,  Y10S47/01

Abstract: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinese (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFR).

Abstract translation: 转基因病原体抗性生物体，其基因组含有至少两种不同基因，在活性启动子控制下具有病原体抑制作用。 该生物体的特征在于协同的病原体抑制作用。 当基因编码chitinese（ChiS，ChiG），葡聚糖酶（GluG），蛋白质合成抑制剂（PSI）和抗真菌蛋白质（AFR））的基因时，这一行动是显而易见的。

公开(公告)号：US06207367B1

公开(公告)日：2001-03-27

申请号：US07525312

申请日：1990-05-18

Applicant: Timothy George Helentjaris ,  Gretchen Jane King

Inventor： Timothy George Helentjaris ,  Gretchen Jane King

IPC: C12Q168

CPC classification number: C12Q1/683 ,  C12Q1/6895 ,  Y10S47/01

Abstract: The invention is in a process of genetic mapping for plant identification and breeding purposes. The process utilitzes restriction fragment technology and detection of polymorphisms to build up a genomic “fingerprint” for a variety or isolate. The “fingerprint” is then compared to “fingerprints” of other varieties or isolates to determine both the degree of relatedness and homozygosity for identification and breeding purposes.

Abstract translation: 本发明是用于植物鉴定和繁殖目的的遗传作图的过程。 该过程有利于限制性片段技术和多态性检测，以建立多样性或分离株的基因组“指纹”。 然后将“指纹”与其他品种或分离物的“指纹图谱”进行比较，以确定鉴定和繁殖目的的相关性和纯合度。

公开(公告)号：US6111168A

公开(公告)日：2000-08-29

申请号：US810898

申请日：1997-03-05

Applicant: Victor Raboy

Inventor： Victor Raboy

IPC: A01H1/04 ,  A01H5/10 ,  A01H3/00 ,  A01H4/00 ,  A01H5/00 ,  C12N15/00

CPC classification number: A01H1/04 ,  A01H5/10 ,  Y10S47/01 ,  Y10S47/09 ,  Y10T436/16

Abstract: Single-gene, non-lethal mutations responsible for low phytic acid-containing seeds are selectable by means of a method for assaying seeds which are otherwise phenotypically, or nearly phenotypically, normal. Maize mutants having from 20% to 95% reductions in kernel phytic acid phosphorus compared to the wild-type, without any noticeable reduction in total phosphorus, were isolated by this method. Mutants obtained in accordance with the invention are useful for developing commercial, low phytic acid seed, plant lines.

Abstract translation: 负责含低含植酸的种子的单基因，非致死突变是通过用于分析否则表型或几乎表型正常的种子的方法来选择的。 通过该方法分离出与野生型相比，植酸磷减少20％至95％的玉米突变体，而没有显着降低总磷。 根据本发明获得的突变体可用于开发商业，低植酸种子，植物品系。

公开(公告)号：US5920003A

公开(公告)日：1999-07-06

申请号：US843930

申请日：1997-04-17

Applicant: Michael A. Hall

Inventor： Michael A. Hall

IPC: A01H5/10 ,  A01H5/00 ,  A01H1/00 ,  A01H4/00 ,  C12N5/04

CPC classification number: A01H5/10 ,  Y10S47/01

Abstract: According to the invention, there is provided inbred corn plants, separately designated 01DIB2 and 01IZB2. This invention thus relates to the plants, seeds and tissue cultures of the fore-mentioned inbred corn plants and to methods for producing a corn plant produced by crossing one of the inbred plants with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing any of the inbred plants 01DIB2 or 01IZB2 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plants 01DIB2 and 01IZB2, and also to the RFLP and genetic isozyme typing profiles of such inbred corn plants.

Abstract translation: 根据本发明，提供了近交玉米植物，分别指定为01DIB2和01IZB2。 因此，本发明涉及前述的近交玉米植物的植物，种子和组织培养物以及通过将近交植物与其自身或与另一种玉米植物（例如另一种近交系）杂交而产生的玉米植物的方法。 本发明还涉及通过将任何近交植物01DIB2或01IZB2与另一种玉米植物（例如另一种近交系）杂交并与相关物种杂交而产生的玉米种子和植物。 本发明还涉及近交玉米植物01DIB2和01IZB2的近交和杂种遗传互补，以及这些近交玉米植物的RFLP和遗传同工酶分型谱。

公开(公告)号：US5889188A

公开(公告)日：1999-03-30

申请号：US757286

申请日：1996-11-27

Applicant: Mark David Hoffbeck

Inventor： Mark David Hoffbeck

IPC: A01H5/10 ,  A01H5/00 ,  A01H1/00 ,  A01H4/00 ,  C12N5/06

CPC classification number: A01H5/10 ,  Y10S47/01

Abstract: An inbred maize line, designated PH0B4, the plants and seeds of inbred maize line PH0B4, methods for producing a maize plant produced by crossing the inbred line PH0B4 with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH0B4 with another maize line or plant.

Abstract translation: 称为PH0B4的近交玉米品系，近交玉米品系PH0B4的植物和种子，通过将自交系PH0B4与其他玉米植物杂交而生产的玉米植物的方法，以及杂交玉米种子和通过交叉近交系产生的植物 另一个玉米生产线或植物生产线PH0B4。

公开(公告)号：US5866763A

公开(公告)日：1999-02-02

申请号：US719841

申请日：1996-09-30

Applicant: Michael B. Buendgen

Inventor： Michael B. Buendgen

IPC: A01H5/10 ,  A01H5/00 ,  A01H4/00 ,  C12N5/04 ,  A01H14/00

CPC classification number: A01H5/10 ,  Y10S47/01

Abstract: Broadly this invention provides inbred corn line ZS01220. The methods for producing a corn plant by crossing the inbred line ZS01220 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01220 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01220 with at least one other corn line.

Abstract translation: 本发明广泛地提供近交玉米线ZS01220。 通过使近交系ZS01220交叉生产玉米植物的方法包括在本发明中。 此外，本发明涉及近交系ZS01220的各个部分，包括可培养细胞。 本发明涉及杂交玉米种子和通过将近交系ZS01220与至少一种其它玉米品系交叉产生的植物。