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    RECOMBINANT LIPIDATED PsaA PROTEIN, METHODS OF PREPARATION AND USE
    1.
    发明申请
    RECOMBINANT LIPIDATED PsaA PROTEIN, METHODS OF PREPARATION AND USE 失效
    重组脂肪酸PsaA蛋白，制备和使用方法

    公开(公告)号：US20100260802A1

    公开(公告)日：2010-10-14

    申请号：US12619357

    申请日：2009-11-16

    申请人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    发明人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    IPC分类号： A61K39/09 ,  C07H21/04 ,  A61P31/04 ,  C07K14/315 ,  C12N15/63 ,  C12P21/00

    CPC分类号： C07K14/3156 ,  A61K39/00 ,  C07K2319/00 ,  Y02A50/401

    摘要： The present invention relates to recombinant lipidated PsaA proteins and recombinant constructs from which such lipidated PsaA proteins may be expressed. The invention relates further to lipidated PsaA proteins in which lipidation is effected by the use of a heterologous leader sequence derived from the ospA gene of Borrelia burgdorferi, which leader sequence is joined in translational reading frame with the psaA structural gene. The invention also provides methods of preparation of lipidated PsaA proteins and use of such proteins in immunological compositions. Also provided are vaccines comprising immunogenic lipidated PsaA proteins and methods of use of such vaccines in the prevention and treatment of S. pneumoniae infection.

    摘要翻译： 本发明涉及重组脂化的PsaA蛋白和重组构建体，其中可以表达这种脂质化的PsaA蛋白。 本发明进一步涉及脂质化PsaA蛋白，其中通过使用源自博氏疏螺旋体的ospA基因的异源前导序列来实现脂化，该引导序列与psaA结构基因连接在翻译阅读框中。 本发明还提供了脂质化PsaA蛋白的制备方法和这些蛋白质在免疫组合物中的应用。 还提供了包含免疫原性脂化PsaA蛋白的疫苗以及这种疫苗在预防和治疗肺炎链球菌感染中的使用方法。

    Influenza virus subunit conjugates
    3.
    发明授权
    Influenza virus subunit conjugates 失效
    流感病毒亚单位缀合物

    公开(公告)号：US5612037A

    公开(公告)日：1997-03-18

    申请号：US280463

    申请日：1994-07-26

    申请人： Robert C. Huebner ,  Maurice W. Harmon

    发明人： Robert C. Huebner ,  Maurice W. Harmon

    IPC分类号： A61K39/02 ,  A61K39/145 ,  A61K39/385 ,  C07K1/10 ,  C07K14/11

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/6037 ,  A61K2039/6075 ,  A61K2039/6087 ,  A61K2039/627 ,  C12N2760/16134

    摘要： Conjugates of HA protein of influenza virus suitable for formulation as a vaccine for obtaining a strong immune response to the HA protein are formed by separating whole HA protein from the influenza virus by detergent extraction or by providing whole HA protein by recombinant procedure, treating the HA protein with hydroxylamine to form free sulfhydryl groups in the cytoplasmic domain of the protein, and cross-linking the free sulfhydryl group-containing HA protein to itself using a bis-maleimide linker or to a maleimide-modified diphtheria toxoid, tetanus toxoid or influenza NP protein or other carrier molecule. The procedure is applicable to other proteins which can be separated from a cellular material, such as a virus, and which contain thioester bonds convertible to sulfhydryl groups.

    摘要翻译： 通过用洗涤剂提取法分离整个HA蛋白与流感病毒，或者通过重组方法提供整个HA蛋白，通过重组方法处理HA来形成适合制备作为用于获得对HA蛋白的强免疫应答的疫苗的流感病毒的HA蛋白质的缀合物 蛋白质与羟胺在蛋白质的细胞质结构域中形成游离的巯基，并且使用双马来酰亚胺接头或马来酰亚胺修饰的白喉类毒素，破伤风类毒素或流感性NP将含游离巯基的HA蛋白质自身交联 蛋白质或其他载体分子。 该方法适用于可以从诸如病毒的细胞物质分离的其它蛋白质，并且其含有可转化为巯基的硫酯键。

    Recombinant lipidated PsaA protein, methods of preparation and use
    4.
    发明授权
    Recombinant lipidated PsaA protein, methods of preparation and use 失效
    重组脂质化PsaA蛋白，制备和使用方法

    公开(公告)号：US07960535B2

    公开(公告)日：2011-06-14

    申请号：US12619357

    申请日：2009-11-16

    申请人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    发明人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K49/00

    CPC分类号： C07K14/3156 ,  A61K39/00 ,  C07K2319/00 ,  Y02A50/401

    摘要： The present invention relates to recombinant lipidated PsaA proteins and recombinant constructs from which such lipidated PsaA proteins may be expressed. The invention relates further to lipidated PsaA proteins in which lipidation is effected by the use of a heterologous leader sequence derived from the ospA gene of Borrelia burgdorferi, which leader sequence is joined in translational reading frame with the psaA structural gene. The invention also provides methods of preparation of lipidated PsaA proteins and use of such proteins in immunological compositions. Also provided are vaccines comprising immunogenic lipidated PsaA proteins and methods of use of such vaccines in the prevention and treatment of S. pneumoniae infection.

    摘要翻译： 本发明涉及重组脂化的PsaA蛋白和重组构建体，其中可以表达这种脂质化的PsaA蛋白。 本发明进一步涉及脂质化PsaA蛋白，其中通过使用源自博氏疏螺旋体的ospA基因的异源前导序列来实现脂化，该引导序列与psaA结构基因连接在翻译阅读框中。 本发明还提供了脂质化PsaA蛋白的制备方法和这些蛋白质在免疫组合物中的应用。 还提供了包含免疫原性脂化PsaA蛋白的疫苗以及这种疫苗在预防和治疗肺炎链球菌感染中的使用方法。

    Recombinant lipidated PsaA protein, methods of preparation and use
    6.
    发明授权
    Recombinant lipidated PsaA protein, methods of preparation and use 失效
    重组脂质化PsaA蛋白，制备和使用方法

    公开(公告)号：US07635486B1

    公开(公告)日：2009-12-22

    申请号：US09600057

    申请日：1999-01-14

    申请人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    发明人： Edwin W. Ades ,  George M. Carlone ,  Barun K. De ,  Jacquelyn S. Sampson ,  Robert C. Huebner

    IPC分类号： A61K39/02 ,  A61K45/00 ,  A61K39/09

    CPC分类号： C07K14/3156 ,  A61K39/00 ,  C07K2319/00 ,  Y02A50/401

    摘要： The present invention relates to recombinant lipidated PsaA proteins and recombinant constructs from which such lipidated PsaA proteins may be expressed. The invention relates further to lipidated PsaA proteins in which lipidation is effected by the use of a heterologous leader sequence derived from the ospA gene of Borrelia burgdorferi, which leader sequence is joined in translational reading frame with the psaA structural gene. The invention also provides methods of preparation of lipidated PsaA proteins and use of such proteins in immunological compositions. Also provided are vaccines comprising immunogenic lipidated PsaA proteins and methods of use of such vaccines in the prevention and treatment of S. pneumoniae infection.

    摘要翻译： 本发明涉及重组脂化的PsaA蛋白和重组构建体，其中可以表达这种脂质化的PsaA蛋白。 本发明进一步涉及脂质化PsaA蛋白，其中通过使用源自博氏疏螺旋体的ospA基因的异源前导序列来实现脂化，该引导序列与psaA结构基因连接在翻译阅读框中。 本发明还提供了脂质化PsaA蛋白的制备方法和这些蛋白质在免疫组合物中的应用。 还提供了包含免疫原性脂化PsaA蛋白的疫苗以及这种疫苗在预防和治疗肺炎链球菌感染中的使用方法。

    Biological particulate matter analogue
    7.
    发明授权
    Biological particulate matter analogue 失效
    生物颗粒物类似物

    公开(公告)号：US07179596B2

    公开(公告)日：2007-02-20

    申请号：US10248775

    申请日：2003-02-17

    申请人： Andrew E. Page ,  Kelly L Brown ,  David S. Alburty ,  Robert C. Huebner

    发明人： Andrew E. Page ,  Kelly L Brown ,  David S. Alburty ,  Robert C. Huebner

    IPC分类号： C12Q1/68 ,  C12Q1/04 ,  C07K4/04 ,  C07H21/02 ,  C07H21/04

    CPC分类号： G01N33/543 ,  C12Q1/04 ,  C12Q1/6813 ,  C12Q1/6851 ,  C12Q2545/113 ,  C12Q2563/155

    摘要： The present invention provides a biological particle analogue, or biological analogue, that simulates a chosen biological organism or compound. The biological analogue includes a first portion that is not, in and of itself, recognized by the biological detection system, and a second portion, which provides the properties necessary for recognition by the detection system, carried by the first portion. The biological analogue is constructed in such a way as to include some important characteristics of the chosen biological organism or compound, while excluding other undesirable characteristics of the chosen biological organism or compound. The present invention is useful in testing a variety of biological detection systems.

    摘要翻译： 本发明提供了模拟所选择的生物体或化合物的生物颗粒类似物或生物类似物。 该生物学模拟物包括本身不被生物检测系统识别的第一部分和提供由第一部分携带的由检测系统识别所必需的性质的第二部分。 构建生物类似物以包括所选择的生物体或化合物的一些重要特征，同时排除选择的生物体或化合物的其它不良特征。 本发明可用于测试各种生物检测系统。

    Compositions and methods for administering Borrelia DNA
    9.
    发明授权
    Compositions and methods for administering Borrelia DNA 失效
    组合物和施用Borrelia DNA的方法

    公开(公告)号：US06451769B1

    公开(公告)日：2002-09-17

    申请号：US09173053

    申请日：1998-10-15

    申请人： Robert C. Huebner ,  Jon A. Norman ,  Xiaowu Liang ,  Kristin R. Carner ,  Alan G. Barbour ,  Catherine J. Luke

    发明人： Robert C. Huebner ,  Jon A. Norman ,  Xiaowu Liang ,  Kristin R. Carner ,  Alan G. Barbour ,  Catherine J. Luke

    IPC分类号： A61K3170

    CPC分类号： C07K14/20 ,  A61K39/00 ,  C12N15/85 ,  Y02A50/401 ,  Y10S977/804

    摘要： Disclosed is a vaccine against Lyme Disease or its causative agent Borrelia burgdorferi (sensu stricto or sensu lato) containing a plasmid a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding Borrelia OspA or OspB, and a DNA encoding a terminator. Disclosed too is an immunogenic composition against Lyme Disease or its causative agent Borrelia burgdorferi (sensu stricto or sensu lato) containing a plasmid comprising a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding a Borrelia OspC, and a DNA encoding a terminator. And, methods for making and using such vaccines and the immunogenic composition are also disclosed.

    摘要翻译： 公开了一种针对莱姆病的疫苗或其致病剂博氏疏螺旋体（sensu stricto或sensu lato），其含有编码用于在哺乳动物细胞中驱动表达的启动子的DNA质粒，编码促进原核蛋白分泌/释放的前导肽的DNA 来自哺乳动物细胞的序列，编码Borrelia OspA或OspB的DNA，以及编码终止子的DNA。 还公开了针对莱姆病或其致病因子伯氏疏螺旋体（sensu stricto或sensu lato）的免疫原性组合物，其包含质粒，其包含编码用于在哺乳动物细胞中驱动表达的启动子的DNA，编码促进分泌/释放的前导肽的DNA 来自哺乳动物细胞的原核蛋白质序列，编码疏螺旋体OspC的DNA和编码终止子的DNA。 并且还公开了制备和使用这些疫苗的方法和免疫原性组合物。