公开(公告)号：US20150368660A1

公开(公告)日：2015-12-24

申请号：US14762128

申请日：2014-01-22

申请人： Hugh MASON ,  Charles ARNTZEN ,  Sun Hee ROSENTHAL ,  Sean WINKLE ,  Andy DIAMOS ,  THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andy Diamos

IPC分类号： C12N15/82 ,  C12N7/00 ,  C07K16/28

CPC分类号： C12N15/8258 ,  C07K16/2887 ,  C07K2317/13 ,  C07K2317/14 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8218 ,  C12N2750/12043

摘要： A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.

摘要翻译： 描述了含有两个或更多个非竞争性复制子的单个载体或多个单独的载体，用于在烟草本生叶中短暂表达利妥昔单抗的重链和轻链。 还描述了将这些亚基蛋白正确组装到功能性低聚结构中以优化表达。 该系统通过消除对非竞争病毒的需要，推进植物瞬时表达技术，从而增强了用于在植物细胞中生产利妥昔单抗的多复制子单载体系统的实际商业应用。