Method of Constructing Animal Having Cancer Cells Transplanted Thereinto
    6.
    发明申请
    Method of Constructing Animal Having Cancer Cells Transplanted Thereinto 审中-公开
    构建具有移植癌细胞的动物的方法

    公开(公告)号:US20080289052A1

    公开(公告)日:2008-11-20

    申请号:US10591612

    申请日:2005-03-04

    Abstract: A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.

    Abstract translation: 首先制备细胞培养载体,其涂覆在表面上,其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后,将培养液调整为聚合物具有更强的水合力的温度,由此分离培养的癌细胞。 然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。

    Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof
    8.
    发明申请
    Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof 有权
    培养的牙周膜细胞片,其制备方法及其使用方法

    公开(公告)号:US20080118474A1

    公开(公告)日:2008-05-22

    申请号:US11587427

    申请日:2005-04-25

    Abstract: A cultured periodontal ligament cell sheet is produced by a process comprising culturing cells on a cell culture support composed of a base material whose surface is coated with a temperature-responsive polymer having an upper limit or lower limit critical dissolution temperature in water of 0 to 80° C. under specified conditions, (1) regulating the temperature of a culture fluid to the upper limit critical dissolution temperature or higher or to the lower limit critical temperature or lower, (2) allowing a cultured periodontal ligament cell sheet resulting from the culturing to adhere to a carrier and (3) detaching the sheet intact together with the carrier. This cultured periodontal ligament cell sheet has a syndesmotic microstructure, exhibits extremely high bioadherence to the dental root surface and realizes high density transplantation of target cells and positive reconstruction of periodontal tissues. Moreover, through lamination of cell sheets to be transplanted and donation of a three-dimensional polarity, highly efficient reconstruction of an adhesion apparatus can be accomplished and clinical application thereof to moderate periodontitis, severe peridontitis, gingival recession and the like is highly promising.

    Abstract translation: 培养的牙周韧带细胞片通过以下方法制备:将细胞培养在由表面涂覆有在水中的上限或下限临界溶解温度为0〜80的温度敏感性聚合物的基材构成的细胞培养支持体上 ℃,(1)将培养液的温度调节至上限临界溶解温度或更高或下限临界温度或更低,(2)允许培养产生的培养的牙周膜细胞片 粘贴在载体上,(3)与载体一起分离片材。 这种培养的牙周膜细胞片具有相似的微观结构,对牙根表面具有极高的生物相干性,实现了靶细胞的高密度移植和牙周组织的积极重建。 此外,通过层叠待移植的细胞片和三维极性的提供,可以实现粘附装置的高效重建,并且其适度的牙周炎,严重的周围炎,牙龈退缩等的临床应用是非常有希望的。

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