公开(公告)号：US10603410B2

公开(公告)日：2020-03-31

申请号：US15321357

申请日：2015-06-24

Applicant: Kaoru Washio ,  Isao Ishikawa ,  Teruo Okano ,  GNERAL INC.

Inventor： Kaoru Washio ,  Isao Ishikawa ,  Teruo Okano ,  Takao Hanawa ,  Yusuke Tsutsumi ,  Yuka Tsumanuma ,  Supreda Suphanantachat ,  Kosei Yano

IPC: A61L27/38 ,  A61L27/32 ,  A61C8/00 ,  A61L27/06 ,  A61L27/16 ,  A61L27/36 ,  A61C8/02

Abstract: A complex of implant and cultured periodontal ligament cell sheet, that can be satisfactorily stabilized into the bone through periodontal ligament tissue, is provided. A fixture of the inserted implant is coated with calcium phosphate and the cultured periodontal ligament cell sheet is brought into intimate contact with the implant.

公开(公告)号：US09289454B2

公开(公告)日：2016-03-22

申请号：US13822945

申请日：2011-09-14

Applicant: Hidekazu Sekine ,  Tatsuya Shimizu ,  Teruo Okano

Inventor： Hidekazu Sekine ,  Tatsuya Shimizu ,  Teruo Okano

IPC: A61K35/44 ,  A61K35/34 ,  A61K35/36 ,  C12N5/077 ,  A61L27/36 ,  A61L27/38

CPC classification number: A61K35/44 ,  A61K35/34 ,  A61K35/36 ,  A61L27/3604 ,  A61L27/3839 ,  A61L27/3895 ,  C12N5/0657 ,  C12N2502/28 ,  C12N2513/00

Abstract: A method for producing multilayered cell sheets, including producing a vascular bed which includes an artery-vein loop and in which a capillary vascular network is constructed; layering cell sheets on the vascular bed; and perfusing a culture medium in vitro to construct a vascular network in the cell sheets. The production method enables vascular networks to be constructed in cell sheets and enables thick multilayered cell sheets to foe easily produced by layering the cell sheets. Such thick multilayered cell sheets are useful as in vivo tissue-like products for regenerative medicine for various tissues and for evaluation of drugs and the like.

Abstract translation: 一种生产多层细胞片的方法，包括制备包括动静脉环的血管床，其中构成毛细血管网络; 在血管床上分层细胞片; 并在体外灌注培养基以在细胞片中构建血管网络。 该制造方法能够在细胞片中构建血管网络，能够通过层叠细胞片容易地制造厚层多层细胞片。 这种厚的多层细胞片可用作用于各种组织的再生医学的体内组织样产品和药物等的评估。

公开(公告)号：US20140056859A1

公开(公告)日：2014-02-27

申请号：US14009018

申请日：2013-03-30

Applicant: Teruo Okano ,  Tatsuya Shimizu ,  Jun Yamashita ,  Hidetoshi Masumoto

Inventor： Teruo Okano ,  Tatsuya Shimizu ,  Jun Yamashita ,  Hidetoshi Masumoto

IPC: A61K35/34 ,  A61K35/44 ,  A61K9/70

CPC classification number: A61K35/34 ,  A61K9/70 ,  A61K35/44 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/54 ,  A61L2300/414 ,  A61L2430/20 ,  C12N5/0657 ,  C12N5/0697 ,  C12N2501/01 ,  C12N2501/04 ,  C12N2501/165 ,  C12N2502/1329 ,  C12N2502/1347 ,  C12N2502/28 ,  C12N2506/02 ,  A61K2300/00

Abstract: The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.

Abstract translation: 本发明提供使用源自胚胎干细胞的一组细胞产生心肌片的方法。 该方法的特征在于混合来自胚胎干细胞的Flk / KDR阳性细胞，心肌细胞，内皮细胞和壁细胞，并培养混合细胞。 此外，心肌片可用作心脏病的治疗剂，因为VEGF从片材中释放出来。

公开(公告)号：US08642338B2

公开(公告)日：2014-02-04

申请号：US10544542

申请日：2003-02-06

Applicant: Masayuki Yamato ,  Teruo Okano

Inventor： Masayuki Yamato ,  Teruo Okano

IPC: C12N11/08 ,  C12N5/00 ,  C12N5/02

CPC classification number: C12N5/0621 ,  A61K35/12

Abstract: An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a process comprising the steps of cultivating cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer having an upper or lower critical dissolution temperature of 0-80° C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter, (1) adjusting the temperature of the culture solution to either above the upper critical dissolution temperature or below the lower critical dissolution temperature, and further optionally (2) bringing the cultured anterior ocular segment related cell sheet or three-dimensional structure into close contact with a polymer membrane, and (3) detaching the sheet or three-dimensional structure together with the polymer membrane.

Abstract translation: 前眼部相关的细胞片或三维结构只有几个结构缺陷，因为它们已经被回收，保留了细胞和基质之间的细胞间质结构和基底膜样蛋白。 前眼部相关细胞片或三维结构的制备方法包括以下步骤：在细胞培养支持物上培养细胞，所述细胞培养载体包含其表面被温度响应性聚合物覆盖的基底，所述温度响应聚合物具有上或下临界溶解温度为0 -80℃，任选地通过常规方法分层培养细胞层，然后，（1）将培养溶液的温度调节至高于上临界溶解温度或低于临界溶解温度 ，进一步任选地（2）使培养的眼前段相关细胞片或三维结构与聚合物膜紧密接触，和（3）与聚合物膜一起分离片或三维结构。

公开(公告)号：US20130289723A1

公开(公告)日：2013-10-31

申请号：US13823240

申请日：2011-09-15

Applicant: Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

Inventor： Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

IPC: A61F2/18

CPC classification number: A61F2/18 ,  A61F2002/183 ,  A61F2310/00365 ,  A61K35/12 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3839 ,  A61L27/3886 ,  A61L2430/14 ,  C12N5/062 ,  C12N2533/30

Abstract: The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cell sir the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80 ° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

Abstract translation: 本发明的目的是获得中耳粘膜替代物，其置于中耳骨表面上，肉芽组织和骨骼的增生以及成纤维细胞的发育 中耳的中耳被抑制，并获得在表层中保留纤毛的中耳粘膜样细胞片，包括在涂覆有聚合物的细胞培养基质上培养鼻上皮细胞，其水合力在0至 80℃，其中在聚合物的水合力弱的温度范围内培养细胞，然后将温度改变到水合力强的温度以回收培养的细胞片。

公开(公告)号：US20120252110A1

公开(公告)日：2012-10-04

申请号：US13432658

申请日：2012-03-28

Applicant: Mitsuhiro OURA ,  Teruo OKANO ,  Tatsuya SHIMIZU ,  Hirotsugu KUBO ,  Sunao TAKEDA

Inventor： Mitsuhiro OURA ,  Teruo OKANO ,  Tatsuya SHIMIZU ,  Hirotsugu KUBO ,  Sunao TAKEDA

IPC: C12M1/26 ,  C12M1/34

CPC classification number: C12M33/06 ,  C12M29/26 ,  C12M41/14 ,  C12M41/48

Abstract: A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

Abstract translation: 细胞培养装置包括：隔离器，其中设置容纳装有包含待培养细胞的培养液的细胞培养箱的无菌空间; 取样单元，其构造为对细胞培养器中的培养液进行取样; 输送流动路径，无菌空间的内部和无菌空间的外部通过其彼此连通，输送流路被配置为将输送流动路径中的流动限制到从无菌空间的内部指向的方向 空间朝无菌空间外面; 以及培养液输送部，其构造成经由所述输送流路将取样的培养液输送到所述无菌空间的外部。

公开(公告)号：US20120214250A1

公开(公告)日：2012-08-23

申请号：US13400106

申请日：2012-02-19

Applicant: Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

Inventor： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

IPC: G01N21/59

CPC classification number: G01N21/80 ,  C12M41/26 ,  G01N21/3151 ,  G01N31/221 ,  G01N2201/0627 ,  G01N2201/1211

Abstract: A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances ; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.

Abstract translation: 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在测量区域的另一侧上接收发射光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。

公开(公告)号：US20110207220A1

公开(公告)日：2011-08-25

申请号：US13125491

申请日：2009-08-11

Applicant: Teruo Okano ,  Masayuki Yamato ,  Tatsuya Shimizu ,  Masatoshi Kuroda

Inventor： Teruo Okano ,  Masayuki Yamato ,  Tatsuya Shimizu ,  Masatoshi Kuroda

IPC: C12N5/071

CPC classification number: C12N11/02 ,  C12M23/20 ,  C12M33/00 ,  C12N5/0062 ,  C12N2535/10 ,  C12N2539/10

Abstract: This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.

Abstract translation: 本发明提供了一种电池图案回收工具，其包括具有易于粘附处理的表面的基材层，设置在基材层上并具有进行硅烷处理的表面的温度响应性聚合物层和细胞粘附抑制材料 提供在温度敏感聚合物层上的层。 根据本发明，可以在针对细胞的微创条件下稳定且可靠地保持细胞图案，能够快速地恢复细胞图案。

公开(公告)号：US07470424B2

公开(公告)日：2008-12-30

申请号：US10484839

申请日：2002-07-25

Applicant: Kazunori Kataoka ,  Hidenori Otsuka ,  Teruo Okano ,  Yukio Nagasaki ,  Yasuhiro Horiike

Inventor： Kazunori Kataoka ,  Hidenori Otsuka ,  Teruo Okano ,  Yukio Nagasaki ,  Yasuhiro Horiike

IPC: C12N5/02 ,  C12N5/06

CPC classification number: C12N5/0671 ,  C12N5/0697 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2535/10

Abstract: Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.

Abstract translation: 将实质细胞培养在培养的内皮细胞或培养的成纤维细胞上，这些成纤维细胞已经被特定的亲水性聚合物的表面分离并且已被图案化。 因此本发明提供了含有如此形成的培养实质细胞的图案化球体的培养物。 该培养物保持长时间对实质细胞特异的功能。

公开(公告)号：US20080289052A1

公开(公告)日：2008-11-20

申请号：US10591612

申请日：2005-03-04

Applicant: Teruo Okano ,  Akihiko Kikuchi ,  Masayuki Yamato ,  Akira Masuda

Inventor： Teruo Okano ,  Akihiko Kikuchi ,  Masayuki Yamato ,  Akira Masuda

IPC: G01N33/15 ,  A01K67/027

CPC classification number: A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K49/0008 ,  C12N5/0693

Abstract: A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.

Abstract translation: 首先制备细胞培养载体，其涂覆在表面上，其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后，将培养液调整为聚合物具有更强的水合力的温度，由此分离培养的癌细胞。 然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。