公开(公告)号：US11988666B2

公开(公告)日：2024-05-21

申请号：US17639391

申请日：2020-09-01

申请人： FUJIREBIO INC.

发明人： Tomonori Nishii ,  Kazunori Okada ,  Naoki Ishikawa ,  Kumiko Iida ,  Shintaro Yagi ,  Katsumi Aoyagi

IPC分类号： G01N33/574

CPC分类号： G01N33/57484 ,  G01N2333/471 ,  G01N2333/4724

摘要： A lectin-binding substance measurement method is a method for measuring a lectin-binding substance in a sample, and includes a measuring step of bringing a blocked labeled lectin including a water-soluble carrier made of a first water-soluble polymer and a labeling substance and a lectin immobilized on the water-soluble carrier into contact with the sample.

公开(公告)号：US11604199B2

公开(公告)日：2023-03-14

申请号：US16765018

申请日：2018-11-16

申请人： FUJIREBIO INC.

发明人： Tatsunari Yoshimi ,  Kyoko Teruyama

IPC分类号： G01N33/74

摘要： In a method for immunologically quantifying a steroid, it is possible to exclude a steroid from cyclodextrin when a standard solution containing the steroid included in cyclodextrin is treated with a surfactant, which thereby suppresses the deviation in behavior of the antigen-antibody reaction between the specimen (test sample) and the standard solution, and then significantly improves the accuracy of steroid quantification.

公开(公告)号：US11454632B2

公开(公告)日：2022-09-27

申请号：US16768419

申请日：2018-11-22

申请人： FUJIREBIO INC.

发明人： Chiharu Ohue ,  Kumiko Iida ,  Katsumi Aoyagi ,  Shintaro Yagi

IPC分类号： G01N33/576 ,  G01N33/563 ,  G01N33/53

摘要： Disclosed is a highly sensitive assay method and assay kit for HBsAg, which do not require treatment with a strong acid or alkali in the sample pretreatment, and which is less susceptible to influences by the autoantibodies. The assay method for hepatitis B virus s antigen in a sample separated from a living body includes: a pretreatment step of mixing a sample with a pretreatment reagent containing a reducing agent, to reduce hepatitis B virus s antigen; and an immunoassay step of subjecting the pretreated sample to an immunoassay of hepatitis B virus s antigen using at least one antibody or antigen-binding fragment thereof capable of antigen-antibody reaction with a reduced peptide composed of the amino acids at positions 98 to 179 of hepatitis B virus s antigen.

公开(公告)号：US20220236155A1

公开(公告)日：2022-07-28

申请号：US17612116

申请日：2020-05-19

申请人： H.U. Group Research Institute G.K. ,  FUJIREBIO INC.

发明人： Ran GU ,  Fumi ASAI ,  Tatsutoshi INUZUKA

IPC分类号： G01N1/40 ,  G01N1/34 ,  G01N33/53

摘要： The present invention provides a technique for reducing contamination with impurities in a system of operating an extracellular vesicle. More specifically, the present invention provides a method of washing an extracellular vesicle and the like. The method includes washing the extracellular vesicle with a nonionic surfactant that is a chain compound containing a structure represented by —O—(—CH2—CH2—O—)x—H, wherein x is a value defined in the description.

公开(公告)号：US20200049712A1

公开(公告)日：2020-02-13

申请号：US16342744

申请日：2017-10-17

申请人： FUJIREBIO INC.

发明人： Takuya SAKYU ,  Kumiko HAMANO ,  Ryujiro HARA

IPC分类号： G01N33/573 ,  C07K16/40 ,  G01N33/563

摘要： An antibody which shows higher reactivity to active renin than to inactive renin wherein use of the antibody led to successful development of an immunological measurement method in which the measurement value strongly correlates with renin activity even in the presence of a renin inhibitor.

公开(公告)号：US20160202249A1

公开(公告)日：2016-07-14

申请号：US14912764

申请日：2014-08-20

申请人： FUJIREBIO INC.

发明人： Tatsuki MATSUNO ,  Mariko HORIIKE

IPC分类号： G01N33/53

CPC分类号： G01N33/5308 ,  C12Q1/6804 ,  C12Q1/6834 ,  C12Q1/6837 ,  G01N33/54393 ,  G01N33/68 ,  C12Q2565/507 ,  C12Q2525/117 ,  C12Q2525/173 ,  C12Q2545/107 ,  C12Q2565/519

摘要： An object of the present invention is to provide a technique that suppresses a background value of a detection signal to construct an immunoassay system for detecting a modified nucleobase. Specifically, the present invention provides a method for measuring a modified nucleobase including incubating a nucleic acid sample and a capture probe in a solution and measuring a modified nucleobase using an antibody against the modified nucleobase in the presence of an absorbent polynucleotide in the obtained solution. The present invention also provides a kit for measuring a modified nucleobase including a capture probe, an absorbent polynucleotide, and an antibody against a modified nucleobase.

摘要翻译： 本发明的目的是提供抑制检测信号的背景值构建用于检测修饰的核碱基的免疫测定系统的技术。 具体地，本发明提供了一种测定修饰核碱基的方法，包括在溶液中孵育核酸样品和捕获探针，并在获得的溶液中在吸收性多核苷酸存在下，使用针对经修饰的核碱基的抗体测量修饰的核碱基。 本发明还提供了用于测量包含捕获探针，吸收性多核苷酸和针对修饰的核碱基的抗体的修饰核碱基的试剂盒。

公开(公告)号：US20160154017A1

公开(公告)日：2016-06-02

申请号：US14952436

申请日：2015-11-25

申请人： FUJIREBIO INC. ,  JEOL LTD.

发明人： Kazuyuki OGURI ,  Tomohiro ENDO ,  Toshiaki OHTAKE ,  Hidenobu KAWADA ,  Takahiro MATSUMOTO

IPC分类号： G01N35/00 ,  B65G17/20 ,  G01N33/53 ,  B25J15/10 ,  G01N35/10 ,  G01N35/04 ,  B25J15/00 ,  B25J15/08

CPC分类号： G01N35/0099 ,  B25J15/0028 ,  B25J15/08 ,  B25J15/103 ,  B65G17/20 ,  G01N33/5302 ,  G01N35/025 ,  G01N35/04 ,  G01N35/10 ,  G01N2035/0465

摘要： [PROBLEMS] It is an object to provide a gripping mechanism that enables inhibition of possible static electricity and contamination in a gripping object such as a reaction container, while enabling the orientation of the gripping object to be stabilized.[MEANS TO SOLVE PROBLEMS] The gripping mechanism is a gripping mechanism for gripping a cuvette T, the gripping mechanism comprising: a sandwiching section 50 for holding the cuvette T in a laterally sandwiching manner; a pressing section 60 for pressing downward an upper end surface of the cuvette T held in a sandwiching manner by the sandwiching section 50, wherein the pressing section 60 is arranged such that, with the cuvette T being held in a sandwiching manner by the sandwiching section 50, a central axis of the cuvette T along a vertical direction coincides mutually with a central axis of the pressing section 60 in the vertical direction.

摘要翻译： 本发明的目的是提供一种夹持机构，其能够抑制可能的静电和诸如反应容器之类的夹持物体中的污染，同时能够使夹持物体的取向稳定。 解决问题的手段夹持机构是用于夹持试管T的夹持机构，夹持机构包括：用于将试管T保持在横向夹持方式的夹持部50; 按压部60，其通过夹持部50以夹持方式向下方挤压反应杯T的上端面，其中按压部60配置成使得由所述夹持部夹持所述试管T的夹持部 如图50所示，反应杯T的中心轴在垂直方向上与按压部60的中心轴线垂直方向相互重合。

公开(公告)号：US20150369826A1

公开(公告)日：2015-12-24

申请号：US14766329

申请日：2014-02-18

申请人： TFB, INC.

发明人： Toru TETSUMOTO ,  Minoru HIRATA

IPC分类号： G01N33/72 ,  G01N33/53

CPC分类号： G01N33/723 ,  G01N33/5306 ,  G01N33/531

摘要： Disclosed is novel means for favorably maintaining the dispersed state of a latex reagent in an immunoassay kit and preventing occurrence of coagulation/sedimentation due to temperature changes so that the reagent is protected from deterioration in the measurement performance, which means is also applicable to an immunoagglutination assay kit containing a latex reagent aliquoted in small amounts. The immunoassay reagent of the present invention comprises unsensitized latex particles and trimethylglycine in a solvent. The concentration of trimethylglycine in the reagent is preferably 5 to 10 w/v %. The reagent can be favorably used as an immunoassay reagent for, e.g., hemoglobin A1c.

摘要翻译： 公开了一种有利地将乳胶试剂的分散状态保持在免疫测定试剂盒中的新方法，并且防止由于温度变化引起的凝结/沉淀的发生，从而保护试剂免受测量性能的劣化，这意味着也适用于免疫凝集 测定试剂盒含有少量等分的胶乳试剂。 本发明的免疫测定试剂在溶剂中含有未敏化的胶乳颗粒和三甲基甘氨酸。 试剂中三甲基甘氨酸的浓度优选为5〜10w / v％。 该试剂可以有利地用作例如血红蛋白A1c的免疫测定试剂。

公开(公告)号：US20150219638A1

公开(公告)日：2015-08-06

申请号：US14420122

申请日：2013-08-06

申请人： FUJIREBIO INC. ,  EIDIA Co., Ltd.

发明人： Kentaro Yamaguchi ,  Katsumi Aoyagi ,  Azusa Terao

IPC分类号： G01N33/543

CPC分类号： G01N33/54306 ,  C07K14/745 ,  G01N33/57438 ,  G01N33/86 ,  G01N2333/745

摘要： Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.

摘要翻译： 公开了PIVKA-II测定方法，其比常规方法获得更好的血浆 - 血浆相关性，以及试剂和试剂盒。 根据本发明的PIVKA-II测量方法包括使用特异性结合凝血酶原片段F1或其抗原结合片段的抗-F1抗体和抗F2的混合物通过免疫测定在样品中测量PIVKA-II 特异性结合凝血酶原片段F2或其抗原结合片段的抗体; 和与PIVKA-II或其抗原结合片段特异性结合的抗PIVKA-II抗体。

公开(公告)号：US08785199B2

公开(公告)日：2014-07-22

申请号：US13202720

申请日：2010-02-18

申请人： Yoshiyuki Hotta

发明人： Yoshiyuki Hotta

IPC分类号： C12N5/00 ,  C12N5/077

CPC分类号： C12N5/0653

摘要： Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum.

摘要翻译： 公开了在无血清或低血清培养基条件下，有效诱导哺乳动物体细胞干细胞分化为具有脂肪细胞特征的细胞的培养基，添加剂和方法。 用于诱导哺乳动物体细胞干细胞分化为脂肪细胞的培养基包括用于培养哺乳动物细胞的基础培养基，用于诱导哺乳动物体细胞干细胞分化为脂肪细胞的试剂，生物素，内皮细胞分化基因（Edg）家族的配体 受体，维生素C和HEPES，其培养基是无血清或含有低浓度的血清。