发明申请
US20160202249A1 METHOD FOR MEASURING MODIFIED NUCLEOBASE USING ABSORBENT POLYNUCLEOTIDE AND KIT FOR SAME
审中-公开
使用吸收性多核苷酸和其它试剂盒测定改性核酸酶的方法
- 专利标题： METHOD FOR MEASURING MODIFIED NUCLEOBASE USING ABSORBENT POLYNUCLEOTIDE AND KIT FOR SAME
- 专利标题（中）： 使用吸收性多核苷酸和其它试剂盒测定改性核酸酶的方法
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申请号： US14912764申请日： 2014-08-20
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公开(公告)号： US20160202249A1公开(公告)日： 2016-07-14
- 发明人: Tatsuki MATSUNO , Mariko HORIIKE
- 申请人： FUJIREBIO INC.
- 申请人地址： JP Shinjuku-ku, Tokyo
- 专利权人： FUJIREBIO INC.
- 当前专利权人： FUJIREBIO INC.
- 当前专利权人地址： JP Shinjuku-ku, Tokyo
- 优先权： JP2013-171665 20130821
- 国际申请： PCT/JP2014/071705 WO 20140820
- 主分类号： G01N33/53
- IPC分类号： G01N33/53
摘要：
An object of the present invention is to provide a technique that suppresses a background value of a detection signal to construct an immunoassay system for detecting a modified nucleobase. Specifically, the present invention provides a method for measuring a modified nucleobase including incubating a nucleic acid sample and a capture probe in a solution and measuring a modified nucleobase using an antibody against the modified nucleobase in the presence of an absorbent polynucleotide in the obtained solution. The present invention also provides a kit for measuring a modified nucleobase including a capture probe, an absorbent polynucleotide, and an antibody against a modified nucleobase.
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