公开(公告)号：US12038440B2

公开(公告)日：2024-07-16

申请号：US17639400

申请日：2020-09-01

申请人： FUJIREBIO INC.

发明人： Kazunori Okada ,  Kumiko Iida ,  Shintaro Yagi ,  Katsumi Aoyagi

IPC分类号： G01N33/574

CPC分类号： G01N33/57484 ,  G01N2333/471

摘要： A lectin-binding substance measurement method is a method for measuring a lectin-binding substance in a sample, and includes: a capturing step of bringing a capture carrier including a water-insoluble carrier and a molecule immobilized on the water-insoluble carrier, the molecule being a molecule capable of capturing a lectin-binding substance, into contact with the sample to cause the capture carrier to capture the lectin-binding substance; a washing step of removing contaminants unbound to the capture carrier; a releasing step of releasing the lectin-binding substance from the capture carrier to obtain a prepared sample; and a measuring step of measuring the lectin-binding substance in the prepared sample by using a lectin.

公开(公告)号：US11802867B2

公开(公告)日：2023-10-31

申请号：US16332647

申请日：2017-09-12

申请人： FUJIREBIO INC.

发明人： Mizuho Sakaki ,  Yoshiyuki Kitamura ,  Shintaro Yagi ,  Katsumi Aoyagi

IPC分类号： G01N33/53 ,  G01N33/68

CPC分类号： G01N33/5306 ,  G01N33/6887

摘要： A measurement method and a measurement reagent for cardiac troponin which enable accurate measurement of the amount of cardiac troponin contained in a sample irrespective of the type of the sample and the presence or absence of other components are disclosed. The measurement method for cardiac troponin, wherein cardiac troponin in a sample separated from a body is measured by an immunoassay, includes a pretreatment step of mixing the sample separated from a body with a pretreatment liquid containing one or both of a surfactant and an acidifier. The reagent for immunoassay of cardiac troponin includes a pretreatment liquid containing one or both of a surfactant and an acidifier.

公开(公告)号：US11768209B2

公开(公告)日：2023-09-26

申请号：US16330549

申请日：2017-09-05

申请人： FUJIREBIO INC.

发明人： Kosuke Yamamoto ,  Yoshiyuki Kitamura ,  Shintaro Yagi ,  Katsumi Aoyagi

IPC分类号： G01N33/78

CPC分类号： G01N33/78

摘要： A measurement method and a measurement reagent for thyroglobulin, which enable measurement of a more accurate amount of thyroglobulin by a single test without being influenced by interference of anti-thyroglobulin antibody, are disclosed. The measurement method for thyroglobulin, wherein thyroglobulin in a sample separated from a body is measured by an immunoassay, includes a pretreatment step of mixing the sample separated from a body with a pretreatment liquid containing one or both of a surfactant and an acidifier. The reagent for immunoassay of thyroglobulin includes a pretreatment liquid containing one or both of a surfactant and an acidifier.

公开(公告)号：US11169162B2

公开(公告)日：2021-11-09

申请号：US16084634

申请日：2017-03-13

申请人： FUJIREBIO INC.

发明人： Noriko Katagiri ,  Shintaro Yagi

IPC分类号： G01N33/88 ,  G01N33/543

摘要： Disclosed is a method of measuring the prostaglandin E main urinary metabolite (PGE-MUM), in which a mixture solution of a urine sample treated with alkali can directly be subjected to an antigen-antibody reaction system in an immunoassay of PGE-MUM, without neutralization and dilution followed by dispensation. The method of measuring PGE-MUM includes the steps of: a) mixing a urine sample with an alkaline aqueous solution, and b) subjecting the mixture solution resulting from a) to an immunoassay using a bicyclo PGE-MUM-immobilized or anti-bicyclo PGE-MUM antibody-immobilized solid phase to measure PGE-MUM in the urine sample, wherein the immunoassay is performed in a weakly-acidic basal buffer solution in the presence of a second pH buffering agent which exerts a buffering effect in the basic range and is different from the pH buffering agent contained in the basal buffer solution, and in the presence of a cationic surfactant.

公开(公告)号：US11988666B2

公开(公告)日：2024-05-21

申请号：US17639391

申请日：2020-09-01

申请人： FUJIREBIO INC.

发明人： Tomonori Nishii ,  Kazunori Okada ,  Naoki Ishikawa ,  Kumiko Iida ,  Shintaro Yagi ,  Katsumi Aoyagi

IPC分类号： G01N33/574

CPC分类号： G01N33/57484 ,  G01N2333/471 ,  G01N2333/4724

摘要： A lectin-binding substance measurement method is a method for measuring a lectin-binding substance in a sample, and includes a measuring step of bringing a blocked labeled lectin including a water-soluble carrier made of a first water-soluble polymer and a labeling substance and a lectin immobilized on the water-soluble carrier into contact with the sample.

公开(公告)号：US11454632B2

公开(公告)日：2022-09-27

申请号：US16768419

申请日：2018-11-22

申请人： FUJIREBIO INC.

发明人： Chiharu Ohue ,  Kumiko Iida ,  Katsumi Aoyagi ,  Shintaro Yagi

IPC分类号： G01N33/576 ,  G01N33/563 ,  G01N33/53

摘要： Disclosed is a highly sensitive assay method and assay kit for HBsAg, which do not require treatment with a strong acid or alkali in the sample pretreatment, and which is less susceptible to influences by the autoantibodies. The assay method for hepatitis B virus s antigen in a sample separated from a living body includes: a pretreatment step of mixing a sample with a pretreatment reagent containing a reducing agent, to reduce hepatitis B virus s antigen; and an immunoassay step of subjecting the pretreated sample to an immunoassay of hepatitis B virus s antigen using at least one antibody or antigen-binding fragment thereof capable of antigen-antibody reaction with a reduced peptide composed of the amino acids at positions 98 to 179 of hepatitis B virus s antigen.