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102 条记录 (耗时 0.019 秒)

    REVERSE TRANSCRIPTASE FOR NUCLEIC ACID SEQUENCING
    81.
    发明申请
    REVERSE TRANSCRIPTASE FOR NUCLEIC ACID SEQUENCING 有权

    公开(公告)号：US20210139884A1

    公开(公告)日：2021-05-13

    申请号：US17120030

    申请日：2020-12-11

    申请人： ELEMENT BIOSCIENCES, INC.

    发明人： Matthew KELLINGER ,  Molly HE ,  Tyler LOPEZ

    IPC分类号： C12N15/10 ,  C12N9/12

    摘要： Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.

    SPATIALLY RESOLVED SURFACE CAPTURE OF NUCLEIC ACIDS
    85.
    发明公开
    SPATIALLY RESOLVED SURFACE CAPTURE OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20240052398A1

    公开(公告)日：2024-02-15

    申请号：US18450200

    申请日：2023-08-15

    申请人： Element Biosciences, Inc.

    发明人： Michael PREVITE ,  Shawn LEVY

    IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

    CPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

    摘要： The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and/or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

    Compositions and methods for pairwise sequencing
    90.
    发明授权
    Compositions and methods for pairwise sequencing 有权

    公开(公告)号：US11535892B1

    公开(公告)日：2022-12-27

    申请号：US17377279

    申请日：2021-07-15

    申请人： Element Biosciences, Inc.

    发明人： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

    IPC分类号： C12Q1/6853 ,  C12Q1/6874

    摘要： The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.