公开(公告)号：US20150184208A1

公开(公告)日：2015-07-02

申请号：US14408696

申请日：2013-06-12

申请人： Novozymes A/S

发明人： Lars Oestergaard ,  Lisbeth Kalum ,  Henrik Lund

IPC分类号： C12P7/64 ,  C12N9/02 ,  C11D3/386 ,  C12N9/08

CPC分类号： C12P7/6409 ,  C11D3/38636 ,  C11D3/38654 ,  C11D3/3945 ,  C12N9/0065 ,  C12N9/0069 ,  C12Y111/02001 ,  C12Y113/11012

摘要： The invention relates to enzymatic methods for reducing the amount of fatty acid hydroperoxides in a fatty acid containing fat/oil product.

摘要翻译： 本发明涉及用于减少含脂肪酸的脂/油产品中的脂肪酸氢过氧化物的量的酶法。

公开(公告)号：US20150184204A1

公开(公告)日：2015-07-02

申请号：US14656963

申请日：2015-03-13

申请人： Xyleco, Inc.

发明人： Marshall MEDOFF ,  Thomas Craig MASTERMAN ,  Seul-a BAE ,  Kelly WALLICK

IPC分类号： C12P7/14

CPC分类号： C12P7/14 ,  C12P5/02 ,  C12P5/023 ,  C12P5/026 ,  C12P7/10 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P7/6409 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

摘要： Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, compositions and methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, a cellulase and a second enzyme system including an amylase to produce an intermediate or product, e.g., by fermentation.

摘要翻译： 加工生物质（例如，植物生物质，动物生物质和城市废物生物质）以产生有用的中间体和产品，例如能量，燃料，食品或材料。 例如，描述了可以使用诸如纤维素和/或木质纤维素材料的原料，纤维素酶和包括淀粉酶的第二酶系统的组合物和方法来产生中间体或产物，例如通过发酵。

公开(公告)号：US20150176041A1

公开(公告)日：2015-06-25

申请号：US14548880

申请日：2014-11-20

申请人： Synthetic Genomics, Inc.

发明人： Benjamin M. Griffin ,  Spiros Kambourakis

IPC分类号： C12P7/64 ,  C12N9/02

CPC分类号： C12P7/6409 ,  C12N9/001 ,  C12N9/0071 ,  C12Y114/14001

摘要： The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.

摘要翻译： 本发明提供了生产二羧酸的方法。 所述方法包括用酶孵育脂肪酸或烃底物以产生二羧酸产物。 该酶作用在底物上以产生已经过度氧化并经历C-C键裂解的产物。 在一些实施方案中，具有这些有用特征的酶是细胞色素P450酶的突变体，例如CYP102类的酶或其突变体。 本发明提供了酶，其中这些所需特征可以在单一酶中发现，因此在一些实施方案中，可以通过单一酶的作用进行该方法。

公开(公告)号：US20150143584A1

公开(公告)日：2015-05-21

申请号：US14547327

申请日：2014-11-19

申请人： BASF PLANT SCIENCE GMBH

发明人： Heiko A. Haertel ,  Garima Bhatt

IPC分类号： C07K14/415 ,  C12P7/64 ,  C12N15/82 ,  C07K16/16

CPC分类号： C07K14/415 ,  C07K16/16 ,  C12N9/00 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/93 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12P7/6409

摘要： This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to Arabidopsis thaliana, Brassica napus, Glycine max and Oryza sativa nucleic acid sequences encoding sugar and lipid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods fir manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.

摘要翻译： 本发明一般涉及编码与种子储存化合物在植物中的存在有关的蛋白质的核酸序列。 更具体地，本发明涉及编码糖和脂质代谢调节蛋白的拟南芥，甘蓝型油菜，甘氨酸和水稻核酸序列，以及这些序列在转基因植物中的用途。 特别地，本发明涉及冷冻操作糖相关化合物和提高油水平和改变植物和种子中的脂肪酸组成的方法。 本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

公开(公告)号：US20150140640A1

公开(公告)日：2015-05-21

申请号：US14361603

申请日：2012-11-29

申请人： Kiverdi, Inc.

发明人： John S. Reed ,  Peter Dalla-Betta ,  Itzhak Kurek ,  Christer Jansson ,  Michael Siani-Rose ,  Lisa Dyson

IPC分类号： C12N1/20

CPC分类号： C12N1/20 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

摘要： Disclosed herein are microorganisms capable of growing on crude glycerol and/or glycerol and/or methanol, or combinations thereof. In some embodiments the microorganisms are knallgas bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of converting crude glycerol and/or glycerol and/or methanol produced as byproduct of processes including but not limited to biodiesel production, into organic carbon molecules such as triacylglycerol useful for industrial processes including but not limited to the production of additional biodiesel. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in oleochemicals, jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel and/or oleochemical production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, methyl esters, ethyl esters, alkyl esters, with carbon chains between five and twenty four carbon atoms long.

摘要翻译： 本文公开了能够生长在粗甘油和/或甘油和/或甲醇或其组合上的微生物。 在一些实施方案中，微生物是产生或分泌至少10重量％的脂质的疱疹细菌。 还公开了将作为包括但不限于生物柴油生产的方法的副产物生产的粗甘油和/或甘油和/或甲醇转化为有机碳分子，例如可用于工业过程的有机碳分子，例如三酰基甘油，包括但不限于生产额外的生物柴油 。 还公开了制造化学品或生产用于油化学品，喷气燃料，柴油燃料和生物柴油燃料的化学品的前体的方法。 用于燃料和/或油脂化学生产的化学物质的示例性化学物质或前体是烷烃，烯烃，炔烃，脂肪酸醇，脂肪酸醛，甲酯，乙酯，烷基酯，碳链长度在五至二十四个碳原子之间。

公开(公告)号：US20150125914A1

公开(公告)日：2015-05-07

申请号：US14506491

申请日：2014-10-03

申请人： SOLAZYME, INC.

发明人： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Jeffrey L. Moseley

IPC分类号： C12P7/64 ,  C11C1/00 ,  C11D9/00

CPC分类号： C12P7/64 ,  C11C1/002 ,  C11D9/00 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

摘要翻译： 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP去饱和酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20150068108A1

公开(公告)日：2015-03-12

申请号：US14239228

申请日：2012-08-17

申请人： Gustavo Enrique Schujman ,  Diego De Mendoza

发明人： Gustavo Enrique Schujman ,  Diego De Mendoza

IPC分类号： C12P7/64 ,  C10L1/02

CPC分类号： C12P7/649 ,  C10L1/026 ,  C10L2200/0476 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/6409 ,  C12P7/6436 ,  Y02E50/13

摘要： The present invention describes bacterial strains CECT 7968, CECT 7969 and NCIMB 42026 of the species B. subtilis, capable of expressing the heterologous synthetic mutated genes: pdc and adhB, originating from Z. mobilis, 'tesA, originating from E. coli, and atfl, originating from Acinetobacter sp. ADP1. Furthermore, said strains may overexpress at least one of the genes of the ACC (acetyl-CoA carboxylase) and acyl-CoA synthetase enzymatic complexes. The use of said strains produces an increase in the production of biofuel, preferably biodiesel from glycerin as the carbon source. Moreover, the present invention describes the use of said bacterial strains for the production of said biofuel, biodiesel, from glycerin, as well as a process for synthesising biofuel, preferably biodiesel, using the strains described in the present invention and the biofuel duly obtained.

摘要翻译： 本发明描述了能够表达源自运动发酵单胞菌（t.A）的源自大肠杆菌的异源合成突变基因pdc和adhB的枯草芽孢杆菌菌种CECT7968，CECT7969和NCIMB 42026，以及 atfl，源自不动杆菌属 ADP1。 此外，所述菌株可能过表达ACC（乙酰辅酶A羧化酶）和酰基辅酶A合成酶酶复合物的至少一种基因。 所述菌株的使用产生生物燃料的生产，优选来自甘油作为碳源的生物柴油的生产。 此外，本发明描述了使用所述细菌菌株从甘油生产所述生物燃料，生物柴油，以及使用本发明所述的菌株和适当获得的生物燃料合成生物燃料，优选生物柴油的方法。

公开(公告)号：US20150064757A1

公开(公告)日：2015-03-05

申请号：US14390350

申请日：2013-04-02

申请人： Derek L. Greenfield ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Vikranth Arlagadda ,  Sungwon Lee ,  Xuezhi Li ,  Zhihao Hu

发明人： Derek L. Greenfield ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Vikranth Arlagadda ,  Sungwon Lee ,  Xuezhi Li ,  Zhihao Hu

IPC分类号： C12P7/04 ,  C12N9/02

CPC分类号： C12N9/0008 ,  C12P7/04 ,  C12P7/6409 ,  C12Y102/99006 ,  Y02P20/52

摘要： The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

摘要翻译： 本公开涉及用于改善重组宿主细胞中脂肪醇生产的变体羧酸还原酶（CAR）酶。

公开(公告)号：US08969605B2

公开(公告)日：2015-03-03

申请号：US13640910

申请日：2011-03-30

申请人： Aldo Bosetti ,  Giuliana Franzosi

发明人： Aldo Bosetti ,  Giuliana Franzosi

IPC分类号： C11B13/00 ,  C12P7/64 ,  C02F1/28 ,  C02F1/42 ,  C02F3/28 ,  C02F3/34 ,  C02F11/18 ,  C10G1/02 ,  C10L3/08 ,  C10L5/46

CPC分类号： C12P7/6409 ,  C02F1/281 ,  C02F1/42 ,  C02F3/28 ,  C02F3/341 ,  C02F3/347 ,  C02F11/18 ,  C02F2301/066 ,  C02F2303/10 ,  C10G1/02 ,  C10G2300/1011 ,  C10G2300/1014 ,  C10L3/08 ,  C10L5/46 ,  Y02E50/10 ,  Y02E50/30 ,  Y02E50/343 ,  Y02P30/20 ,  Y02W10/30 ,  Y02W10/37

摘要： A process for producing bio-oil from municipal solid waste, the process including: a) liquifying municipal solid waste, to obtain a mixture containing an oily phase containing bio-oil, a solid phase, and a first aqueous phase; b) treating the first aqueous phase from a) with an adsorbing material, to obtain a second aqueous phase; c) fermenting the second aqueous phase from b), to obtain a biomass; d) subjecting the biomass obtained in c) to the liquification a). The bio-oil obtained is advantageously used in the production of biofuels for motor vehicles or for the generation of electric energy or heat.

摘要翻译： 一种从城市固体废物生产生物油的方法，该方法包括：a）液化城市固体废物，得到含有含油生物油，固相和第一水相的混合物; b）用吸附材料从a）处理第一水相，得到第二水相; c）从b）发酵第二水相，得到生物质; d）使在c）中获得的生物质经历液化a）。 获得的生物油有利地用于生产用于机动车辆的生物燃料或用于产生电能或热量。

公开(公告)号：US20150057461A1

公开(公告)日：2015-02-26

申请号：US14390768

申请日：2013-04-05

申请人： EHWA UNIVERSITY - UNIVERSITY COLLABORATION FOUNDATION

发明人： Jin Byung Park ,  Ji Won Song ,  Eun Yeong Jeon

IPC分类号： C12P7/64 ,  C07C59/01

CPC分类号： C12P7/6409 ,  C07C59/01 ,  C12N9/0073 ,  C12N15/52 ,  C12P7/04 ,  C12P7/44 ,  C12P7/62 ,  C12P7/6436 ,  Y02E50/17

摘要： The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ω-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids or alcohols in a more safe and economic manner.

摘要翻译： 本发明涉及转化为表达Baeyer-Villiger单加氧酶（BVMO）的转化体，一种生产C5-C14中链ω-羟基脂肪酸的方法，α，ω-二羧酸，ω-氨基脂肪酸或 通过使用转化体的生物转化从C16-C20长链脂肪酸得到的醇，使用BVMO由酮脂肪酸引入到其链中的具有酯基的脂肪酸衍生物的制造方法，以及新的ω-羟基脂肪酸 其通过该方法制备。 降解产物如C5至C14ω-羟基脂肪酸，α，ω-二羧酸，ω-氨基脂肪酸，醇可以通过生物转化使用包含在介质中的C16至C20长链脂肪酸大量生产，使用 能够表达本发明的BVMO的转化体。 因此，它可以广泛用于以更安全和经济的方式生产ω-羟基脂肪酸，α，ω-二羧酸，ω-氨基脂肪酸或醇。