公开(公告)号：US20150068108A1

公开(公告)日：2015-03-12

申请号：US14239228

申请日：2012-08-17

申请人： Gustavo Enrique Schujman ,  Diego De Mendoza

发明人： Gustavo Enrique Schujman ,  Diego De Mendoza

IPC分类号： C12P7/64 ,  C10L1/02

CPC分类号： C12P7/649 ,  C10L1/026 ,  C10L2200/0476 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/6409 ,  C12P7/6436 ,  Y02E50/13

摘要： The present invention describes bacterial strains CECT 7968, CECT 7969 and NCIMB 42026 of the species B. subtilis, capable of expressing the heterologous synthetic mutated genes: pdc and adhB, originating from Z. mobilis, 'tesA, originating from E. coli, and atfl, originating from Acinetobacter sp. ADP1. Furthermore, said strains may overexpress at least one of the genes of the ACC (acetyl-CoA carboxylase) and acyl-CoA synthetase enzymatic complexes. The use of said strains produces an increase in the production of biofuel, preferably biodiesel from glycerin as the carbon source. Moreover, the present invention describes the use of said bacterial strains for the production of said biofuel, biodiesel, from glycerin, as well as a process for synthesising biofuel, preferably biodiesel, using the strains described in the present invention and the biofuel duly obtained.

摘要翻译： 本发明描述了能够表达源自运动发酵单胞菌（t.A）的源自大肠杆菌的异源合成突变基因pdc和adhB的枯草芽孢杆菌菌种CECT7968，CECT7969和NCIMB 42026，以及 atfl，源自不动杆菌属 ADP1。 此外，所述菌株可能过表达ACC（乙酰辅酶A羧化酶）和酰基辅酶A合成酶酶复合物的至少一种基因。 所述菌株的使用产生生物燃料的生产，优选来自甘油作为碳源的生物柴油的生产。 此外，本发明描述了使用所述细菌菌株从甘油生产所述生物燃料，生物柴油，以及使用本发明所述的菌株和适当获得的生物燃料合成生物燃料，优选生物柴油的方法。