公开(公告)号：US20240254398A1

公开(公告)日：2024-08-01

申请号：US18560290

申请日：2022-05-17

申请人： Kiverdi, Inc.

发明人： John Reed ,  David Stout ,  Elisabeth Perea ,  Jin-Ping Lim ,  Jil Geller ,  Dan Robertson ,  Robert Wilson ,  Ripudaman Malhotra

IPC分类号： C10G2/00 ,  C12M1/06 ,  C12M1/34 ,  C12N1/20 ,  C12P1/04 ,  C12R1/01

CPC分类号： C10G2/50 ,  C12M27/02 ,  C12M41/26 ,  C12M41/34 ,  C12M41/40 ,  C12N1/20 ,  C12P1/04 ,  C12R2001/01

摘要： Bioreactors and methods for growing a chemoautotrophic culture of a microorganism, such as a hydrogen-oxidizing or carbon monoxide-oxidizing microorganism, are provided. The bioreactors and methods provide for enhanced growth and productivity of microorganisms that use gaseous sources of carbon and energy and provide an environment for carbon fixing to produce organic molecules of interest and/or biomass. The present bioreactors and methods achieve enhanced growth with respect to cell density, culture duration and/or growth rate, while maintaining safe operating conditions.

公开(公告)号：US10696941B2

公开(公告)日：2020-06-30

申请号：US14839074

申请日：2015-08-28

申请人： Kiverdi, Inc.

发明人： Peter Dalla-Betta ,  John S. Reed

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12N1/20 ,  C12M1/12 ,  C12M1/34 ,  C12M1/36

摘要： Compositions and methods and apparatus for growth and maintenance of microorganisms and/or bioprocesses using one or more gases as electron donors, electron acceptors, carbon sources, or other nutrients, and for a bioprocess that converts hydrogen and carbon dioxide, or syngas, or producer gas into lipid products, bio-based oils, or other biochemical products.

公开(公告)号：US20180346941A1

公开(公告)日：2018-12-06

申请号：US15839785

申请日：2017-12-12

申请人： Kiverdi, Inc.

发明人： Itzhak Kurek ,  Lisa Dyson ,  Christer Jannson ,  John S. Reed

IPC分类号： C12P7/40 ,  C12P5/00 ,  C12N9/88 ,  C12N9/16 ,  C12N9/02 ,  C12P7/64 ,  C12P7/54 ,  C12P7/06 ,  C12P5/02 ,  C12P3/00 ,  C12P1/04 ,  C12N1/20

CPC分类号： C12P7/40 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12P1/04 ,  C12P3/00 ,  C12P5/00 ,  C12P5/023 ,  C12P7/065 ,  C12P7/54 ,  C12P7/649 ,  Y02E50/13 ,  Y02E50/17 ,  Y02E50/343

摘要： Compositions and methods for a hybrid biological and chemical process utilizing chemotrophic microorganisms that converts syngas and/or gaseous CO2 and/or a mixture of CO2 gas and H2 gas into one or more desaturated hydrocarbons, unsaturated fatty acids, hydroxy acids, or diacids.

公开(公告)号：US09957534B2

公开(公告)日：2018-05-01

申请号：US15233512

申请日：2016-08-10

申请人： Kiverdi, Inc.

发明人： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

IPC分类号： C12N1/20 ,  C12P7/64 ,  C07H21/04 ,  C12N9/16 ,  C12N9/02

CPC分类号： C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

摘要： Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

公开(公告)号：US20240060191A1

公开(公告)日：2024-02-22

申请号：US18210901

申请日：2023-06-16

申请人： Kiverdi, Inc.

发明人： John S. Reed ,  Jil Geller ,  Sonali Hande

IPC分类号： C25B1/04 ,  C12N1/20 ,  C12P1/04 ,  C12P5/00 ,  C12P7/64 ,  C12P13/04 ,  C12P21/02 ,  C12P7/6409 ,  C12P17/16 ,  C12P13/00 ,  C12P25/00

CPC分类号： C25B1/04 ,  C12N1/20 ,  C12P1/04 ,  C12P5/00 ,  C12P7/64 ,  C12P13/04 ,  C12P21/02 ,  C12P7/6409 ,  C12P17/167 ,  C12P13/001 ,  C12P25/00

摘要： Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts.

公开(公告)号：US20220145337A1

公开(公告)日：2022-05-12

申请号：US17525715

申请日：2021-11-12

申请人： Kiverdi, Inc.

发明人： John S. Reed

IPC分类号： C12P7/40 ,  C12P7/16 ,  C12P7/08 ,  C12M1/00 ,  C12N1/20 ,  C12P1/04 ,  C12P3/00 ,  C12P5/02 ,  C12P7/06 ,  C12P7/54 ,  C12P7/649

摘要： The invention described herein presents compositions and methods for a multistep biological and chemical process for the capture and conversion of carbon dioxide and/or other forms of inorganic carbon into organic chemicals including biofuels or other useful industrial, chemical, pharmaceutical, or biomass products. One or more process steps utilizes chemoautotrophic microorganisms to fix inorganic carbon into organic compounds through chemosynthesis. An additional feature described are process steps whereby electron donors used for the chemosynthetic fixation of carbon are generated by chemical or electrochemical means, or are produced from inorganic or waste sources. An additional feature described are process steps for recovery of useful chemicals produced by the carbon dioxide capture and conversion process, both from chemosynthetic reaction steps, as well as from non-biological reaction steps.

公开(公告)号：US20210115481A1

公开(公告)日：2021-04-22

申请号：US16917450

申请日：2020-06-30

申请人： Kiverdi, Inc.

发明人： John S. Reed ,  Itzhak Kurek ,  Henrik Fyrst ,  Lisa Dyson

IPC分类号： C12P13/22 ,  C12P7/64 ,  C12P13/08 ,  C12P5/00 ,  C12P7/18 ,  C12P7/62 ,  C12P13/00 ,  C12P17/10

摘要： Aspects of the invention relate to methods of producing small molecules for industrial application using natural organisms and engineered organisms.

公开(公告)号：US20200172856A1

公开(公告)日：2020-06-04

申请号：US16231908

申请日：2018-12-24

申请人： Kiverdi, Inc.

发明人： John S. Reed ,  Peter Dalla-Betta ,  Itzhak Kurek ,  Christer Jansson ,  Michael Siani-Rose ,  Lisa Dyson

IPC分类号： C12N1/20 ,  C12N9/16 ,  C12N9/02 ,  C12N9/88 ,  C12P7/64 ,  C12P5/02 ,  C12P5/00 ,  C12P7/04

摘要： Disclosed herein are microorganisms capable of growing on crude glycerol and/or glycerol and/or methanol, or combinations thereof. In some embodiments the microorganisms are knallgas bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of converting crude glycerol and/or glycerol and/or methanol produced as byproduct of processes including but not limited to biodiesel production, into organic carbon molecules such as triacylglycerol useful for industrial processes including but not limited to the production of additional biodiesel. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in oleochemicals, jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel and/or oleochemical production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, methyl esters, ethyl esters, alkyl esters, with carbon chains between five and twenty four carbon atoms long.

公开(公告)号：US20190390158A1

公开(公告)日：2019-12-26

申请号：US16481458

申请日：2018-02-04

申请人： Kiverdi, Inc.

发明人： Lisa Dyson ,  John Reed ,  Jil Geller ,  Sonali Hande

IPC分类号： C12N1/20 ,  C12P1/04 ,  C12P13/04 ,  C12P21/02 ,  C12P7/64

摘要： Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

公开(公告)号：US20190144891A1

公开(公告)日：2019-05-16

申请号：US16193961

申请日：2018-11-16

申请人： Kiverdi, Inc.

发明人： Christer Jansson ,  Cody A. Marcus Carr ,  John S. Reed

IPC分类号： C12P5/00 ,  C07K14/245 ,  C07K14/195 ,  C12P15/00 ,  C12N9/88 ,  C12P7/44

摘要： Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.