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    Assay platforms and detection methodology using surface enhanced Raman scattering (SERS) upon specific biochemical interactions
    61.
    发明申请
    Assay platforms and detection methodology using surface enhanced Raman scattering (SERS) upon specific biochemical interactions 审中-公开
    使用表面增强拉曼散射（SERS）进行特异性生化相互作用的测定平台和检测方法

    公开(公告)号：US20080003576A1

    公开(公告)日：2008-01-03

    申请号：US11477675

    申请日：2006-06-30

    申请人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

    发明人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

    IPC分类号： C12Q1/68 ,  G01N33/555

    CPC分类号： G01N33/585 ,  C12Q1/6816 ,  G01N21/658 ,  G01N33/54373 ,  C12Q2563/155 ,  C12Q2565/632

    摘要： The embodiments of the invention are directed to a SERS cluster comprising a capture particle that is at least partially surrounded by analyte molecules, wherein both the capture particle and the analyte molecules surrounding the capture particle are at least partially surrounded by enhancer particles, wherein a majority of the analyte molecules are either sandwiched between capture and enhancer particles or located between junctions of the enhancer particles. The embodiments of the invention also relate to methods of manufacturing and detecting the SERS cluster. The embodiments of the invention also relate to a SERS active particle comprising a tag molecule comprising a Raman active compound and a probe or a linker having a specific biochemical binding capability and to a method for detecting of a target molecule using a SERS active particle having a tag molecule comprising a Raman active compound and a probe or a linker.

    摘要翻译： 本发明的实施方案涉及包含被分析物分子至少部分包围的捕获颗粒的SERS簇，其中捕获颗粒和捕获颗粒周围的分析物分子至少部分地被增强子颗粒包围，其中多数 分析物分子被夹在捕获和增强剂颗粒之间或位于增强剂颗粒的结之间。 本发明的实施例还涉及制造和检测SERS簇的方法。 本发明的实施方案还涉及包含包含拉曼活性化合物的标签分子和具有特定生物化学结合能力的探针或接头的SERS活性颗粒以及使用具有下列化合物的SERS活性颗粒检测靶分子的方法： 标签分子，其包含拉曼活性化合物和探针或接头。

    Detection of chemical analytes by array of surface enhanced Raman scattering reactions
    62.
    发明申请
    Detection of chemical analytes by array of surface enhanced Raman scattering reactions 审中-公开
    通过阵列的表面增强拉曼散射反应检测化学分析物

    公开(公告)号：US20070155020A1

    公开(公告)日：2007-07-05

    申请号：US11305335

    申请日：2005-12-19

    申请人： Xing Su ,  Lei Sun ,  Kung-bin Sung

    发明人： Xing Su ,  Lei Sun ,  Kung-bin Sung

    IPC分类号： G01N33/543 ,  G06F19/00 ,  C12M1/34 ,  C12M3/00

    CPC分类号： G01N33/54373 ,  G01N21/658

    摘要： A device (and methods of using and manufacturing the device) having a substrate; and a plurality of spots comprising surface enhanced Raman scattering (SERS) active particles attached to the substrate, wherein the SERS active particles reflect an incoming Raman signal to produce a reflected Raman signal having a higher intensity than that of the incoming Raman signal are disclosed. Also a device (and methods of using and manufacturing the device) a substrate; and a plurality of spots comprising composite-organic-inorganic-nanoparticles (COINs) attached to the substrate are disclosed.

    摘要翻译： 具有基板的装置（以及使用和制造装置的方法） 以及多个点，其包含附着到基底的表面增强拉曼散射（SERS）活性粒子，其中SERS活性粒子反射进入的拉曼信号以产生具有比进入的拉曼信号的强度更高的强度的反射拉曼信号。 还有一种器件（以及使用和制造器件的方法）衬底; 并且公开了包含附着到基底的复合有机 - 无机纳米粒子（COIN）的多个斑点。

    Programmable molecular barcodes
    63.
    发明申请
    Programmable molecular barcodes 审中-公开
    可编程分子条形码

    公开(公告)号：US20060199216A1

    公开(公告)日：2006-09-07

    申请号：US11430590

    申请日：2006-05-08

    申请人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

    发明人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  G01N33/551

    CPC分类号： B82Y10/00 ,  B82Y5/00 ,  C12Q1/6816 ,  G06K19/06028 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/1025

    摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

    摘要翻译： 本公开涉及用于生产和/或使用分子条形码的方法。 在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。 标签可以附加到骨干和/或分支结构。 条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。 可以通过标签的类型和位置区分不同的条形码。 在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。 标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。 在替代实施例中，条形码可以通过单体单元的聚合来制备。 绑定的条形码可以通过各种成像模式来检测，例如表面等离子体共振，荧光或拉曼光谱。

    Biomolecular analysis by rolling circle amplification and sers detection
    65.
    发明申请
    Biomolecular analysis by rolling circle amplification and sers detection 审中-公开
    通过滚动圆放大和检测器进行生物分子分析

    公开(公告)号：US20060099636A1

    公开(公告)日：2006-05-11

    申请号：US11313108

    申请日：2005-12-19

    申请人： Lei Sun ,  Xing Su

    发明人： Lei Sun ,  Xing Su

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34

    CPC分类号： C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/682 ,  G01N21/658 ,  C12Q2565/632 ,  C12Q2565/518 ,  C12Q2531/125 ,  C12Q2563/179

    摘要： The present methods, compositions and systems are concerned with biomolecule 130 detection, identification and/or quantification by rolling circle amplification (RCA) and Raman detection. In particular embodiments of the invention, the RCA is exponential RCA or linear RCA. In some embodiments of the invention, the Raman detection is SERS or SERRS. The circular DNA template 150, 210, 310 to be amplified may comprise one or more polythymidine 320 residues, resulting in amplification products 170, 230, 250, 330, 410 containing multiple polyadenylate 340, 420 residues. The polyadenylates 340, 420 may be directly detected by Raman detection. Alternatively, one or more Raman labels may be incorporated into the amplification products 170, 230, 250, 330, 410 to facilitate Raman detection. Because of the amplification produced by LRCA or ERCA and the enhanced Raman signal produced by multiple polyadenylates 340, 420 and/or Raman labels, detection of single copy biomolecules 130 is feasible using the disclosed methods, compositions and/or systems.

    摘要翻译： 本方法，组合物和系统涉及通过滚环扩增（RCA）和拉曼检测的生物分子130检测，鉴定和/或定量。 在本发明的特定实施例中，RCA是指数RCA或线性RCA。 在本发明的一些实施方案中，拉曼检测是SERS或SERRS。 要扩增的环状DNA模板150,210,310可以包含一个或多个多胸苷320残基，导致扩增产物170,230,250,330,410含有多个聚腺苷酸340,420个残基。 多聚腺苷酸酯340,420可以通过拉曼检测直接检测。 或者，可以将一个或多个拉曼标记掺入扩增产物170,230,250,330,410以促进拉曼检测。 由于由LRCA或ERCA产生的扩增和由多个多腺苷酸340,420和/或拉曼标记产生的增强的拉曼信号，使用所公开的方法，组合物和/或系统检测单拷贝生物分子130是可行的。

    External modification of composite organic inorganic nanoclusters
    66.
    发明申请
    External modification of composite organic inorganic nanoclusters 有权
    复合有机无机纳米团簇的外部改性

    公开(公告)号：US20060073336A1

    公开(公告)日：2006-04-06

    申请号：US10940698

    申请日：2004-09-13

    申请人： Jingwu Zhang ,  Xing Su ,  Lei Sun

    发明人： Jingwu Zhang ,  Xing Su ,  Lei Sun

    IPC分类号： B32B5/16

    CPC分类号： G01N21/658 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  Y10S977/773 ,  Y10S977/777 ,  Y10S977/783 ,  Y10T428/12049 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： Modified and functionalized metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. For example, modifications include coatings and layers, such as adsorption layers, metal coatings, silica coatings, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A metal that enhances the Raman signal from the organic Raman-active compound is inherent in the nanocluster. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

    摘要翻译： 提供了能够从其中结合的有机拉曼活性分子提供增强的拉曼信号的改性和官能化的金属纳米团簇。 例如，修饰包括涂层和层，例如吸附层，金属涂层，二氧化硅涂层和有机层。 纳米团簇通常被称为COIN（复合有机无机纳米粒子），并且能够作为敏感的报告物进行分析物检测。 从有机拉曼活性化合物增强拉曼信号的金属在纳米簇中是固有的。 可以将多种有机拉曼活性化合物和化合物的混合物掺入纳米团簇中。

    Multiplexed detection of analytes in fluid solution
    67.
    发明申请
    Multiplexed detection of analytes in fluid solution 审中-公开
    流体溶液中分析物的多重检测

    公开(公告)号：US20060033910A1

    公开(公告)日：2006-02-16

    申请号：US10916710

    申请日：2004-08-11

    申请人： Lei Sun ,  Xing Su

    发明人： Lei Sun ,  Xing Su

    IPC分类号： G01J3/44 ,  G01N21/65

    CPC分类号： G01N33/54346 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/658 ,  G01N2021/6482 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

    摘要： Methods and devices for solution-based detection of molecular and cellular analytes in a sample using composite organic-inorganic nanoclusters (COINs) are provided. The nanoclusters include metallic colloids and a Raman-active organic compound. A metal that enhances the Raman signal from the organic compound is inherent in the nanoparticle. Since a wide variety of Raman-active organic compounds can be incorporated into the particle, highly parallel analyte detection can be performed.

    摘要翻译： 提供了使用复合有机 - 无机纳米团簇（COINs）的样品中分子和细胞分析物溶液检测的方法和装置。 纳米团簇包括金属胶体和拉曼活性有机化合物。 增强来自有机化合物的拉曼信号的金属是纳米颗粒中固有的。 由于可以将多种拉曼活性有机化合物掺入到颗粒中，因此可以进行高度平行的分析物检测。

    Methods for using Raman spectroscopy to obtain a protein profile of a biological sample
    68.
    发明申请
    Methods for using Raman spectroscopy to obtain a protein profile of a biological sample 审中-公开
    使用拉曼光谱法获得生物样品的蛋白质谱的方法

    公开(公告)号：US20050250159A1

    公开(公告)日：2005-11-10

    申请号：US11083418

    申请日：2005-03-17

    申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Tae-Woong Koo ,  Selena Chan ,  Andrew Berlin ,  Narayanan Sundararajan

    发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Tae-Woong Koo ,  Selena Chan ,  Andrew Berlin ,  Narayanan Sundararajan

    IPC分类号： G01N21/65 ,  G01N33/58 ,  G01N33/68 ,  G01N33/53 ,  G01N33/00

    CPC分类号： G01N21/658 ,  G01N33/6803 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

    摘要： The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

    摘要翻译： 本发明提供了用于分析生物样品的蛋白质含量的方法，例如获得由特定个体提供的样品的蛋白质谱。 样品中的蛋白质和蛋白质片段基于化学和/或物理性质分离，并在固体基质上或流动液体流中的离散位置保持分离状态。 然后检测拉曼光谱，由离散位置处的分离的蛋白质或片段产生，使得离散位置的光谱提供关于在离散位置处的一种或多种特定蛋白质或片段的结构或身份的信息。 离散位置处的蛋白质或片段可以用金属（例如金或银）涂覆，和/或分离的蛋白质可与化学增强剂接触以提供SERS光谱。 还提供了用于实施本发明的方法和试剂盒。