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公开(公告)号：US20180320210A1
公开(公告)日：2018-11-08
申请号：US16033317
申请日：2018-07-12
IPC分类号： C12P19/02
CPC分类号： C12P19/02 , C12N9/1051 , C12N9/90 , C12N9/92 , C12N15/52 , C12Y204/01049 , C12Y503/01 , C12Y503/01009 , C12Y504/02002 , C12Y504/02005 , C12Y504/02006
摘要： Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
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公开(公告)号：US20180087045A1
公开(公告)日：2018-03-29
申请号：US15562456
申请日：2016-03-30
CPC分类号： C12N15/10 , C07K14/195 , C07K2319/034 , C12N1/06 , C12N9/00 , C12N9/1229 , C12N9/1247 , C12N9/127 , C12N9/22 , C12N15/09 , C12N15/11 , C12N15/111 , C12N15/635 , C12N2310/14 , C12N2330/50 , C12N2840/002 , C12P19/30 , C12P19/34 , C12Q1/68 , C12Q1/6844 , C40B40/08 , C12Q2521/119
摘要： Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.
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公开(公告)号：US09688977B2
公开(公告)日：2017-06-27
申请号：US14451708
申请日：2014-08-05
CPC分类号： C12N9/92 , C12N9/1022 , C12N9/1029 , C12Y202/01001 , C12Y203/01008 , C12Y503/01009
摘要： Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
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公开(公告)号：US20170159058A1
公开(公告)日：2017-06-08
申请号：US15436623
申请日：2017-02-17
IPC分类号： C12N15/52 , C12N9/04 , C12N9/88 , C12P7/16 , C12N9/12 , C12N9/10 , C12N15/70 , C12N9/02 , C12N9/90
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/0073 , C12N9/1022 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/16 , C12P7/40 , C12P7/649 , C12Y101/01244 , C12Y102/01012 , C12Y114/13025 , C12Y202/01001 , C12Y207/01011 , C12Y207/0104 , C12Y207/02003 , C12Y401/02013 , C12Y401/02043 , C12Y402/01011 , C12Y501/03001 , C12Y503/01001 , C12Y503/01027 , Y02E50/10 , Y02E50/13
摘要： The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
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公开(公告)号：US20170096692A1
公开(公告)日：2017-04-06
申请号：US15291943
申请日：2016-10-12
CPC分类号： C12P17/184 , C12N9/0008 , C12N9/0026 , C12N9/0071 , C12N9/1029 , C12N9/1217 , C12N9/93 , C12N15/52 , C12Y102/01041 , C12Y105/99008 , C12Y203/01 , C12Y207/02011 , C12Y603/03004 , C12Y603/04016 , C12Y603/05005 , Y02P20/52
摘要： Provided herein are cell-free systems for generating carbapenems, e.g., a compound of the Formula (I): or salts thereof; wherein , R1, R2, R3, R4, R5 and R6 are defined herein. Also provided are pharmaceutical compositions comprising a compound generated by the inventive cell-free system, and use of these compounds and compositions for the treatment of bacterial infections.
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公开(公告)号：US09611487B2
公开(公告)日：2017-04-04
申请号：US14137524
申请日：2013-12-20
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/0073 , C12N9/1022 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/16 , C12P7/40 , C12P7/649 , C12Y101/01244 , C12Y102/01012 , C12Y114/13025 , C12Y202/01001 , C12Y207/01011 , C12Y207/0104 , C12Y207/02003 , C12Y401/02013 , C12Y401/02043 , C12Y402/01011 , C12Y501/03001 , C12Y503/01001 , C12Y503/01027 , Y02E50/10 , Y02E50/13
摘要： The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
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公开(公告)号：US20150037868A1
公开(公告)日：2015-02-05
申请号：US14451708
申请日：2014-08-05
CPC分类号： C12N9/92 , C12N9/1022 , C12N9/1029 , C12Y202/01001 , C12Y203/01008 , C12Y503/01009
摘要： Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
摘要翻译： 本文的各个方面和实施方案涉及具有至少一种能够被同源蛋白酶灭活的蛋白酶识别序列的重组蛋白质和制备此类蛋白质的方法。 在一些实施方案中，提供了重组磷酸葡萄糖异构酶（Pgi）蛋白。 在其它实施方案中，提供了重组磷酸转乙酰酶（Pta）蛋白。 在其它实施方案中，提供重组转酮醇酶A（TktA）蛋白质。
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