公开(公告)号：US10011831B2

公开(公告)日：2018-07-03

申请号：US15466824

申请日：2017-03-22

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Brittan Pasloske ,  Quoc Hoang

IPC: C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/6806

CPC classification number: C12N15/1096 ,  C12N15/1003 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2521/537 ,  C12Q2521/301 ,  C12Q2521/319 ,  C12Q2521/107

Abstract: The invention relates to methods for the detection of a specific sequence of RNA in a cell or tissue sample. The invention also relates to methods to enzymatically manipulate the RNA in a crude cell lysate in a number of applications.

公开(公告)号：US20180142231A1

公开(公告)日：2018-05-24

申请号：US15568098

申请日：2015-04-20

Applicant: QIAGEN GmbH

Inventor： Johann Kubicek ,  Thorsten Singer ,  Antje-Katrin Sander

IPC: C12N15/10 ,  C12N9/22 ,  C12Q1/6806

CPC classification number: C12N15/1003 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/537 ,  C12Q2523/113

Abstract: The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

公开(公告)号：US20180105808A1

公开(公告)日：2018-04-19

申请号：US15825740

申请日：2017-11-29

Applicant: 10X Genomics, Inc.

Inventor： Tarjei Sigurd Mikkelsen ,  Phillip Belgrader ,  Xinying Zheng

IPC: C12N15/10 ,  C12Q1/68 ,  C12N5/071

CPC classification number: C12N15/1003 ,  C12N5/0602 ,  C12N15/1062 ,  C12N15/1065 ,  C12N15/1096 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2563/159 ,  C12Q2565/514

Abstract: The present disclosure provides methods, compositions and systems for analyzing individual cells or cell populations through a partitioned analysis of contents of individual cells or cell populations, such as cancer cells and cells of the immune system. Individual cells or cell populations may be co-partitioned with processing reagents for accessing cellular contents, and for uniquely identifying the content of a given cell or cell population, and subsequently analyzing the content of the cell and characterizing it as having derived from an individual cell or cell population, including analysis and characterization of nucleic acid(s) from the cell through sequencing.

公开(公告)号：US09938519B2

公开(公告)日：2018-04-10

申请号：US14833836

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Michele R. Stone

IPC: G01N1/34 ,  C12N15/10

CPC classification number: C12N15/1003 ,  G01N1/34

Abstract: The present invention relates to methods and systems for the isolation of DNA on a microfluidic device and the subsequent analysis of the DNA on the microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for performing amplification reactions, such as PCR, and detection, such as thermal melt analysis, on the microfluidic.

公开(公告)号：US20180094254A1

公开(公告)日：2018-04-05

申请号：US15728199

申请日：2017-10-09

Applicant: ABBOTT MOLECULAR INC.

Inventor： Dae Hyun H. Kim ,  Gerard J. Gundling ,  Herbert A. Marble ,  Mark W. Eshoo

IPC: C12N15/10

CPC classification number: C12N15/101 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1013 ,  C12N15/1017

Abstract: Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA.

公开(公告)号：US20180087047A1

公开(公告)日：2018-03-29

申请号：US15279948

申请日：2016-09-29

Applicant: PZM Diagnostics, LLC

Inventor： Peilin Zhang ,  Lawrence M. Minardi

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q2527/125 ,  C12Q2527/137

Abstract: Methods and systems for extracting DNA from a sample are provided. A method includes: lysing red blood cells of the sample using a first solution; dissolving lipids of cellular membranes in the sample using a second solution; and releasing DNA of the sample into a buffer using a third solution.

公开(公告)号：US20180066247A1

公开(公告)日：2018-03-08

申请号：US15696477

申请日：2017-09-06

Applicant: Toshiba Medical Systems Corporation

Inventor： Hiroaki Kushima ,  Nobuo Kawamura ,  Tetsuya Kuwabara

IPC: C12N15/10 ,  B02C19/16

CPC classification number: C12N15/1003 ,  B02C17/14 ,  B02C19/16 ,  C12M45/02

Abstract: According to one embodiment, a biological tissue grinding container includes a container portion and a vibrated portion, and vibration is transmitted to biological tissue from the vibration portion to grind the biological tissue. The vibrated portion includes a contact portion to be brought into direct contact with the biological tissue and defines a chamber which stores the biological tissue to be ground, together with the container portion. The vibrated portion is fixedly supported to be vibratable to the container portion directly or via a support portion provided between the container portion and itself, and has solidity higher than that of the container portion or support portion.

公开(公告)号：US20180044659A1

公开(公告)日：2018-02-15

申请号：US15557502

申请日：2016-04-14

Applicant: Ramot at Tel-Aviv University Ltd. ,  Tsinghua University ,  Intitute of Microbiology, Chinese Academy of Sciences (IMCAS)

Inventor： Ting ZHU ,  Yuval EBENSTEIN ,  Chunbo LOU ,  Wenjun JIANG ,  Xuejin ZHAO ,  Tslil GABRIELI

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/301

Abstract: A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.

公开(公告)号：US20180037883A1

公开(公告)日：2018-02-08

申请号：US15730088

申请日：2017-10-11

Applicant: Covaris, Inc.

Inventor： James A. LAUGHARN, JR. ,  Austin PURDY

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2523/303 ,  G01N1/34 ,  C12Q2523/301 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2531/113 ,  C12Q2565/607

Abstract: Systems and methods for the high yield extraction and recovery of higher quality target molecule(s) (e.g., DNA, RNA, protein, lipids, metabolites) from blood spots. High quality DNA recovered from dried blood spots can be an input source for high throughput analytical methods, such as for polymerase chain reaction (e.g., qPCR) and/or next generation sequencing (NGS). In various embodiments, at least 20.0 nanograms of nucleic acid (e.g., DNA, RNA), or at least 8.0 milligrams of protein, may be extracted and recovered per an amount of dried blood corresponding to approximately 5 microliters of fresh blood. In some embodiments, a majority (e.g., greater than 50%, greater than 60%, up to 95-100%) of the nucleic acid that is extracted and recovered from the blood spot via focused acoustics may be of a quality suitable for amplification via PCR or NGS.

公开(公告)号：US20180031453A1

公开(公告)日：2018-02-01

申请号：US15730172

申请日：2017-10-11

Applicant: Hologic, Inc.

Inventor： Anthony P. Shuber ,  Steven Hecht ,  Erin Coffman ,  Paul MacLean ,  Howard B. Kaufman

IPC: G01N1/30

CPC classification number: G01N1/30 ,  C12N1/066 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/701

Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.