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公开(公告)号：US10508977B2
公开(公告)日：2019-12-17
申请号：US15357872
申请日：2016-11-21
Applicant: Hologic, Inc.
Inventor： Erin Coffman , Steven Hecht , Paul MacLean
Abstract: A wash solution for remediating lubricant contamination in cytological specimens includes a Tris-based alkaline buffer solution. The buffer solution may be prepared in methanol, and may include a proton-rich protein, or a basic amino acid, such as arginine, lysine, or guanidine. A method for remediating a specimen contaminated with lubricant includes using the wash solution and/or adding a salt to the final vial prior to preparing the microscope slide. The method may include adding the wash solution to the specimen, centrifuging the specimen and wash solution to form a supernatant and cell pellet, decanting the supernatant, and adding a preservative solution to the cell pellet. The method may further include adding salt to a vial containing the preservative solution and cell pellet.
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公开(公告)号：US20180031453A1
公开(公告)日：2018-02-01
申请号：US15730172
申请日：2017-10-11
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
IPC: G01N1/30
CPC classification number: G01N1/30 , C12N1/066 , C12N15/1003 , C12N15/101 , C12Q1/6886 , C12Q1/689 , C12Q1/701
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.
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公开(公告)号：US11719607B2
公开(公告)日：2023-08-08
申请号：US17091435
申请日：2020-11-06
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
CPC classification number: G01N1/30 , C12N1/066 , C12N15/101 , C12N15/1003 , C12Q1/689 , C12Q1/6886 , C12Q1/701
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening.
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Patent Agency Ranking
公开(公告)号：US10859475B2
公开(公告)日：2020-12-08
申请号：US15730172
申请日：2017-10-11
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.
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公开(公告)号：US20180143114A1
公开(公告)日：2018-05-24
申请号：US15357872
申请日：2016-11-21
Applicant: Hologic, Inc.
Inventor： Erin Coffman , Steven Hecht , Paul MacLean
IPC: G01N1/34
Abstract: A wash solution for remediating lubricant contamination in cytological specimens includes a Tris-based alkaline buffer solution. The buffer solution may be prepared in methanol, and may include a proton-rich protein, or a basic amino acid, such as arginine, lysine, or guanidine. A method for remediating a specimen contaminated with lubricant includes using the wash solution and/or adding a salt to the final vial prior to preparing the microscope slide. The method may include adding the wash solution to the specimen, centrifuging the specimen and wash solution to form a supernatant and cell pellet, decanting the supernatant, and adding a preservative solution to the cell pellet. The method may further include adding salt to a vial containing the preservative solution and cell pellet.
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公开(公告)号：US20210072125A1
公开(公告)日：2021-03-11
申请号：US17091435
申请日：2020-11-06
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. Surprisingly the presence of beads in the mixture from the partial lysing process does not interfere with slide processing or cellular analysis. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.
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公开(公告)号：US09810610B2
公开(公告)日：2017-11-07
申请号：US14856611
申请日：2015-09-17
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
IPC: G01N1/30
CPC classification number: G01N1/30 , C12N1/066 , C12N15/1003 , C12N15/101 , C12Q1/6886 , C12Q1/689 , C12Q1/701
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.
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公开(公告)号：US20160076980A1
公开(公告)日：2016-03-17
申请号：US14856611
申请日：2015-09-17
Applicant: Hologic, Inc.
Inventor： Anthony P. Shuber , Steven Hecht , Erin Coffman , Paul MacLean , Howard B. Kaufman
IPC: G01N1/30
CPC classification number: G01N1/30 , C12N1/066 , C12N15/1003 , C12N15/101 , C12Q1/6886 , C12Q1/689 , C12Q1/701
Abstract: The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. Surprisingly the presence of beads in the mixture from the partial lysing process does not interfere with slide processing or cellular analysis. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.
Abstract translation: 本公开描述了以部分裂解的方法处理包含细胞的样品的方法。 将细胞暴露于诸如珠粒打浆的过程，其破坏混合物中的一些细胞。 该方法产生适合于细胞形态筛选和遗传筛选的所得样品混合物。 部分裂解的样品的第一部分可以安装在载玻片上并观察非典型细胞和细胞学异常。 部分裂解样品的第二部分可以筛选已知与子宫颈癌风险相关的遗传标记。 令人惊讶的是，来自部分裂解过程的混合物中存在珠不影响载玻片加工或细胞分析。 该方法对宫颈筛查特别有用，其中细胞学和遗传筛选的组合呈现出更完整的宫颈健康情况。 所公开的方法简化了需要两种类型的测定的方案的诊断过程，而不会影响筛选的准确性。
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