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1969 条记录 (耗时 0.054 秒)

    Methods and compositions for the cryopreservation of organs
    42.
    发明授权
    Methods and compositions for the cryopreservation of organs 有权
    器官冷冻保存的方法和组成

    公开(公告)号：US08679735B2

    公开(公告)日：2014-03-25

    申请号：US10571968

    申请日：2004-09-16

    申请人： Gregory M. Fahy ,  Brian Wowk

    发明人： Gregory M. Fahy ,  Brian Wowk

    IPC分类号： A01N1/00

    CPC分类号： A01N1/02 ,  A01N1/0221 ,  A01N1/0284

    摘要： Methods and compositions are provided for the cryopreservation of human organs and tissues. In certain embodiments, Step 1 comprises perfusion with a vitrifiable cryoprotectant solution at a temperature above −10° C. for a time insufficient for the approximate osmotic equilibration of the organ with the solution, followed by cooling the organ to below −10° C. by perfusion with said solution at a reduced temperature. In certain embodiments, Step 2 comprises increasing the concentration of cryoprotectant further at a temperature from −10 to −40° C. In certain embodiments, Step 3 comprises cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant whose temperature is either raised gradually or is held at ≧−15° C. Compositions are provided that allow safe organ perfusion with vitrifiable media at >−10° C. and almost complete avoidance of chilling injury at −20 to −25° C. and that allow slow warming after vitrification without freezing.

    摘要翻译： 为人体器官和组织的低温保存提供了方法和组合物。 在某些实施方案中，步骤1包括在高于-10℃的温度下用可玻璃化的冷冻保护剂溶液灌注不足以使器官与溶液的近似渗透平衡的时间，然后将器官冷却至低于-10℃。 通过在降低的温度下用所述溶液灌注。 在某些实施方案中，步骤2还包括在-10至-40℃的温度下进一步增加冷冻保护剂的浓度。在某些实施方案中，步骤3包括将器官冷却和玻璃化，对其进行再温热，并以可玻璃化浓度 其温度缓慢升高或保持在-15℃以下的冷冻保护剂。提供的组合物允许在-20℃至-25℃几乎完全避免在-20℃至-25℃的冷冻损伤下安全的器官灌注玻璃培养基 并且允许玻璃化后缓慢升温而不冻结。

    Sample chamber adapter, in particular for the cryoconservation of biological samples
    43.
    发明授权
    Sample chamber adapter, in particular for the cryoconservation of biological samples 有权
    样品室适配器，特别用于生物样品的低温保存

    公开(公告)号：US08671783B2

    公开(公告)日：2014-03-18

    申请号：US12997644

    申请日：2009-06-03

    申请人： Günter R. Fuhr ,  Heiko Zimmermann ,  Hagen von Briesen ,  Anja Germann

    发明人： Günter R. Fuhr ,  Heiko Zimmermann ,  Hagen von Briesen ,  Anja Germann

    IPC分类号： G01N1/42

    CPC分类号： B01L9/06 ,  A01N1/02 ,  A01N1/0242 ,  B01L2200/025 ,  B01L2300/022 ,  B01L2300/1894 ,  G01N1/42

    摘要： An adapter device includes a receptacle including at least one receiving element that detachably secures a sample chamber for cryoconservation of a biological sample, and a coupling device that connects the receptacle to a base section which can be secured in a cooling device, wherein the receiving element is 1) immobile in a holding state at a cryoconservation temperature and secures the sample chamber and 2) at least one of deformable and movable in a release state at a temperature above the cryoconservation temperature so that the sample chamber is detachable from the adapter device or can be inserted into the adapter device.

    摘要翻译： 适配器装置包括一个插座，它包括至少一个可拆卸地固定用于生物样品的低温保存的样品室的接收元件，以及一个将接收器连接到可固定在冷却装置中的基部的耦合装置，其中接收元件 是1）在低温保存温度下以保持状态固定并固定样品室，以及2）在高于保存温度的温度下，在释放状态下可变形和可移动的至少一种，使得样品室可从适配器装置或者 可以插入适配器设备。

    Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
    44.
    发明授权
    Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations 有权
    将冷冻解冻的精子分离成携带X染色体和携带Y染色体的人群的方法

    公开(公告)号：US08652769B2

    公开(公告)日：2014-02-18

    申请号：US12853196

    申请日：2010-08-09

    申请人： George E. Seidel ,  Tae Kwang Suh ,  Kehuan Lu ,  David G. Cran

    发明人： George E. Seidel ,  Tae Kwang Suh ,  Kehuan Lu ,  David G. Cran

    IPC分类号： A01N1/02 ,  A61B17/43

    CPC分类号： A01N1/02 ,  A01N1/0226 ,  G01N1/30

    摘要： Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformily stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

    摘要翻译： 用于处理，分离，包装和利用精子的装置，组合物和方法（1），其可以从从雄性哺乳动物收集的先前冷冻的精子样品中得到。 具体来说，即使从先前冷冻的精子和分离技术得到的精子DNA均匀染色（2）精子DNA的技术，即使从先前冷冻的精子样品衍生到具有高纯度的携带X染色体的Y染色体群体中也能分离和分离精子。

    Biological material and methods and solutions for preservation thereof
    48.
    发明授权
    Biological material and methods and solutions for preservation thereof 失效
    生物材料及其保存方法及解决方案

    公开(公告)号：US08512941B2

    公开(公告)日：2013-08-20

    申请号：US13074843

    申请日：2011-03-29

    申请人： Yehudit Natan

    发明人： Yehudit Natan

    IPC分类号： A01N1/02 ,  C12N5/078

    CPC分类号： A61L2/0052 ,  A01N1/02 ,  A01N1/0221 ,  A61L2202/22

    摘要： Provided is a preservation solution for preserving biological material at low temperature including one or more polyphenols and a method for preservation of biological material, the method includes adding the preservation solution to biological material, cooling the biological material and storing it under appropriate storing conditions. The present method may be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, and may be used with any biological material, including cells selected from RBC, WBC, MNC, UCB MNC and bacteria. In the case of RBC, also disclosed is a method for its freezing such that upon thawing, the material has less than 2% free hemoglobin.

    摘要翻译： 本发明提供一种保存低温生物材料的保存液，包括一种或多种多酚和生物材料的保存方法，该方法包括将保存液添加到生物材料中，冷却生物材料并在合适的储存条件下储存。 本方法可用于低温保存或用于低温保存，包括冷冻和冻干，并且可用于任何生物材料，包括选自RBC，WBC，MNC，UCB MNC和细菌的细胞。 在RBC的情况下，还公开了其冷冻的方法，使得在解冻时，该材料具有少于2％的游离血红蛋白。

    APPARATUS AND METHOD FOR MAINTAINING AND/OR RESTORING VIABILITY OF ORGANS
    49.
    发明申请
    APPARATUS AND METHOD FOR MAINTAINING AND/OR RESTORING VIABILITY OF ORGANS 有权
    维护和/或恢复机关可行性的装置和方法

    公开(公告)号：US20130177899A1

    公开(公告)日：2013-07-11

    申请号：US13784326

    申请日：2013-03-04

    申请人： ORGAN RECOVERY SYSTEMS

    发明人： Michael TAYLOR ,  John BRASSIL

    IPC分类号： A01N1/02

    CPC分类号： A01N1/0278 ,  A01N1/02 ,  A01N1/0247

    摘要： An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures. The methods further include perfusing the organ at hypothermic and/or normothermic temperatures with a second perfusate containing a substance for reacting with the organ. Condition of the organ may be automatically monitored, and the perfusion process can be automatically controlled using a control program.

    摘要翻译： 器官灌注装置和方法监测，维持和/或恢复器官的活力并保存器官用于储存和/或运输。 其他装置包括器官输送器，器官盒和器官诊断装置。 该方法包括在低温和/或正常体温下灌注器官。 所述方法还包括用低温和/或正常体温温度的器官灌注含有与器官反应的物质的第二灌注液。 可以自动监测器官的状况，并可以使用控制程序自动控制灌注过程。