公开(公告)号：US07892726B2

公开(公告)日：2011-02-22

申请号：US11628648

申请日：2005-06-07

申请人： Yehudit Natan ,  Tamir Kanias

发明人： Yehudit Natan ,  Tamir Kanias

IPC分类号： A01N1/02 ,  A01K63/00 ,  C12N7/04 ,  C12N13/00

CPC分类号： A61L2/0047 ,  A61L2/0035

摘要： The invention provides a method for the sterilization of a biological preparation comprising desired viable biological entities. The method comprises irradiating a dried (e.g. freeze-dried) biological preparation with ionizing or UV radiation at an intensity and for a duration sufficient to reduce the amount or activity of living-matter contaminants in the biological preparation, the intensity and duration selected such that at least part of the desired biological entities in the sample remains viable. The method of the invention is particularly suitable for the reduction of the amount or activity of contaminants such as bacteria or viruses from biological preparations comprising red blood cells or platelets.

摘要翻译： 本发明提供了一种用于灭菌包含所需生物体的生物制剂的方法。 该方法包括以足以减少生物制剂中生物物质污染物的量或活性的强度和持续时间照射具有电离或UV辐射的干燥（例如冷冻干燥）生物制剂，所选择的强度和持续时间选择使得 样品中至少部分期望的生物实体保持可行。 本发明的方法特别适用于从包含红细胞或血小板的生物制剂中减少诸如细菌或病毒的污染物的量或活性。

公开(公告)号：US08580487B2

公开(公告)日：2013-11-12

申请号：US11102728

申请日：2005-04-11

申请人： Amir Arav ,  Yehudit Natan ,  Saar Yavin ,  Uri Meir ,  Udi Damari

发明人： Amir Arav ,  Yehudit Natan ,  Saar Yavin ,  Uri Meir ,  Udi Damari

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/02

摘要： A method of preserving functionality of an organ. The method includes removing a whole organ and associated vasculature, cryo-preserving the whole organ, allowing a period of time to elapse, thawing the whole organ including vasculature and introducing the whole organ into a recipient so that the organ is supplied with blood by vasculature belonging to the recipient. Further disclosed is a method of preserving fertility of a patient undergoing a treatment expected to cause sterility. The method include removing a whole gonadal organ from the patient, cryo-preserving the whole gonadal organ, conducting the treatment and waiting for an effect thereof to subside, thawing the whole gonadal organ and introducing the whole gonadal organ where it is supplied with blood by the vasculature system of the patient. Further disclosed is a method of assuring a supply of viable gametes for future use, the method includes removing a whole gonadal organ from a patient, cryo-preserving the whole gonadal organ, waiting until viable gametes are desired by the patient, thawing the organ and introducing the gonadal organ into the patient so that it is supplied with blood by a vasculature system belonging to the patient.

公开(公告)号：US20080038818A1

公开(公告)日：2008-02-14

申请号：US11628648

申请日：2005-06-07

申请人： Yehudit Natan ,  Tamir Kanias

发明人： Yehudit Natan ,  Tamir Kanias

IPC分类号： C12N5/00 ,  A61L2/10

CPC分类号： A61L2/0047 ,  A61L2/0035

摘要： The invention provides a method for the sterilization of a biological preparation comprising desired viable biological entities. The method comprises irradiating a dried (e.g. freeze-dried) biological preparation with ionizing or UV radiation at an intensity and for a duration sufficient to reduce the amount or activity of living-matter contaminants in the biological preparation, the intensity and duration selected such that at least part of the desired biological entities in the sample remains viable. The method of the invention is particularly suitable for the reduction of the amount or activity of contaminants such as bacteria or viruses from biological preparations comprising red blood cells or platelets.

摘要翻译： 本发明提供了一种用于灭菌包含所需生物体的生物制剂的方法。 该方法包括以足以减少生物制剂中生物物质污染物的量或活性的强度和持续时间照射具有电离或UV辐射的干燥（例如冷冻干燥）生物制剂，所选择的强度和持续时间选择使得 样品中至少部分期望的生物实体保持可行。 本发明的方法特别适用于从包含红细胞或血小板的生物制剂中减少诸如细菌或病毒的污染物的量或活性。

公开(公告)号：US20070178434A1

公开(公告)日：2007-08-02

申请号：US10588126

申请日：2005-02-02

申请人： Yehudit Natan ,  Tamir Kanias

发明人： Yehudit Natan ,  Tamir Kanias

IPC分类号： A01N1/02

CPC分类号： A61L2/0052 ,  A01N1/02 ,  A01N1/0221 ,  A61L2202/22

摘要： A preservation solution for preserving a biological material at a low temperature including one or more polyphenols, and a method for preserving a biological material are provided. The method includes adding the preservation solution to a biological material, cooling the biological material and storing it under appropriate storing conditions. The method can be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, of any biological material, including cells selected from RBC, WBC, MNC, UCB, MNC, and bacteria. A method is also provided for freezing RBCs such that upon thawing, the RBCs include less than 2% free hemoglobin.

摘要翻译： 提供了一种用于在包括一种或多种多酚的低温下保存生物材料的保存溶液，以及保存生物材料的方法。 该方法包括将保存溶液加入到生物材料中，冷却生物材料并在合适的储存条件下储存。 该方法可用于任何生物材料，包括选自RBC，WBC，MNC，UCB，MNC和细菌的细胞的低温保存或冷冻保存，包括冷冻和冻干。 还提供了用于冷冻RBC的方法，使得在解冻时，RBC包括少于2％的游离血红蛋白。

公开(公告)号：US07935478B2

公开(公告)日：2011-05-03

申请号：US10588126

申请日：2005-02-02

申请人： Yehudit Natan ,  Tamir Kanias

发明人： Yehudit Natan ,  Tamir Kanias

IPC分类号： A01N1/02 ,  A61K9/127 ,  C12N1/04 ,  C12N5/07

CPC分类号： A61L2/0052 ,  A01N1/02 ,  A01N1/0221 ,  A61L2202/22

摘要： A preservation solution for preserving a biological material at a low temperature including one or more polyphenols, and a method for preserving a biological material are provided. The method includes adding the preservation solution to a biological material, cooling the biological material and storing it under appropriate storing conditions. The method can be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, of any biological material, including cells selected from RBC, WBC, MNC, UCB, HSC and bacteria. A method is also provided for freezing RBCs such that upon thawing, the RBCs include less than 2% free hemoglobin.

摘要翻译： 提供了一种用于在包括一种或多种多酚的低温下保存生物材料的保存溶液，以及保存生物材料的方法。 该方法包括将保存溶液加入到生物材料中，冷却生物材料并在合适的储存条件下储存。 该方法可用于任何生物材料（包括选自RBC，WBC，MNC，UCB，HSC和细菌的细胞）的低温保存或冷冻保存，包括冷冻和冻干。 还提供了用于冷冻RBC的方法，使得在解冻时，RBC包括少于2％的游离血红蛋白。

公开(公告)号：US20050175975A1

公开(公告)日：2005-08-11

申请号：US11102728

申请日：2005-04-11

申请人： Amir Arav ,  Yehudit Natan ,  Saar Yavin ,  Uri Meir ,  Udi Damari

发明人： Amir Arav ,  Yehudit Natan ,  Saar Yavin ,  Uri Meir ,  Udi Damari

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/02

摘要： A method of preserving functionality of an organ. The method includes removing a whole organ and associated vasculature, cryo-preserving the whole organ, allowing a period of time to elapse, thawing the whole organ including vasculature and introducing the whole organ into a recipient so that the organ is supplied with blood by vasculature belonging to the recipient. Further disclosed is a method of preserving fertility of a patient undergoing a treatment expected to cause sterility. The method include removing a whole gonadal organ from the patient, cryo-preserving the whole gonadal organ,conducting the treatment and waiting for an effect thereof to subside, thawing the whole gonadal organ and introducing the whole gonadal organ where it is supplied with blood by the vasculature system of the patient. Further disclosed is a method of assuring a supply of viable gametes for future use, the method includes removing a whole gonadal organ from a patient, cryo-preserving the whole gonadal organ, waiting until viable gametes are desired by the patient, thawing the organ and introducing the gonadal organ into the patient so that it is supplied with blood by a vasculature system belonging to the patient.

摘要翻译： 一种维持器官功能的方法。 该方法包括去除整个器官和相关的脉管系统，冷冻保存整个器官，允许一段时间过去，解冻整个器官，包括脉管系统，并将整个器官引入受体，以便通过脉管系统为器官提供血液 属于收件人。 进一步公开的是保持经历预期导致不育的治疗的患者的生育能力的方法。 该方法包括从患者身上清除整个性腺器官，冷冻保存整个性腺器官，进行治疗并等待其效果，使整个性腺器官融化，解冻，并将其中所有供血的性腺器官引入 患者的脉管系统。 进一步公开了一种确保供应可行配子以供将来使用的方法，该方法包括从患者中去除整个性腺器官，冷冻保存整个性腺器官，等待患者期望可食性配子，解冻器官和 将性腺器官引入患者，使其由属于患者的脉管系统供给血液。

公开(公告)号：US20100281886A1

公开(公告)日：2010-11-11

申请号：US12440846

申请日：2007-09-11

申请人： Ginadi Shaham ,  Yehudit Natan ,  Dana Lorber ,  Peter Bartal ,  Yoram Amsalem ,  Yaniv Damari

发明人： Ginadi Shaham ,  Yehudit Natan ,  Dana Lorber ,  Peter Bartal ,  Yoram Amsalem ,  Yaniv Damari

IPC分类号： F25B19/00 ,  F25B29/00

CPC分类号： A01N1/02 ,  A01N1/0257 ,  A01N1/0263 ,  A01N1/0268

摘要： There are provided a system and a method for cryopreserving a liquid biological material disposed in a bag having a longitudinal axis. The system comprises a bag holder for holding the bag, so that the biological material therein has a surface area S, and a volume V, a tank containing a cryogenic fluid, a mechanism for the immersion of the bag holder into the tank along the longitudinal axis, an opening in the tank for insertion therethrough of the bag holder, and a guide member extending from the opening into the tank. There are further provided a system and a method for warming a cryopreserved liquid biological material disposed in a bag. The system comprises a heat source, a warming device having a space for placing the bag therein, connected to the heat source and adapted to transfer heat from the heat source to the bag, and means for maintaining the heat source in heat transfer contact with a cryogenically preserved portion of the material to allow receiving the heat by said cryogenically preserved portion.

摘要翻译： 提供了一种用于冷冻保存设置在具有纵向轴线的袋中的液体生物材料的系统和方法。 该系统包括用于保持袋的袋保持器，使得其中的生物材料具有表面积S，体积V，包含低温流体的罐，用于沿着纵向将袋保持器浸入罐中的机构 所述罐中的开口用于插入所述袋保持器，以及从所述开口延伸到所述罐中的引导构件。 还提供了一种用于加热设置在袋中的冷冻保存的液体生物材料的系统和方法。 该系统包括热源，具有用于将袋放置在其中的空间的加温装置，其连接到热源并适于将热量从热源传送到袋，以及用于将热源保持与热传递接触的装置 材料的低温保存部分以允许由所述低温保存部分接收热量。

公开(公告)号：US20100105133A1

公开(公告)日：2010-04-29

申请号：US11989929

申请日：2006-08-03

申请人： Tamir Kanias ,  Yehudit Natan

发明人： Tamir Kanias ,  Yehudit Natan

IPC分类号： C12N5/0735 ,  C12N5/071 ,  C12N5/074 ,  C12N5/0775

CPC分类号： A01N1/02 ,  A01N1/0221 ,  A61K35/28 ,  A61K35/545

摘要： The present invention generally concerns cell therapy and products for use in such therapy. Particularly, the invention provides a preserved cell preparation essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the preserved cell preparation comprising somatic cells and at least one polyphenol, wherein upon reconstitution of cells in the cell preparation, at least a portion of said stem cells are viable, said portion being sufficient for use of the cell preparation in stem cell therapy. The invention also provides cells reconstituted from preserved somatic cells, and the use of the reconstituted cells in cell therapy. A preferred cell preparation in accordance with the invention comprises stem cells, preferably human stem cells.

摘要翻译： 本发明一般涉及细胞疗法和用于这种治疗的产品。 特别地，本发明提供了基本上不含一种或多种由多元醇，DMSO和低温保护蛋白组成的冷冻保护剂成员的保留细胞制剂，所述保存细胞制剂包含体细胞和至少一种多酚，其中在将 所述细胞制备物，所述干细胞的至少一部分是可存活的，所述部分足以用于干细胞治疗中的细胞制备。 本发明还提供从保存的体细胞重建的细胞，以及在细胞治疗中使用重构的细胞。 根据本发明的优选的细胞制剂包含干细胞，优选人干细胞。

公开(公告)号：US08512941B2

公开(公告)日：2013-08-20

申请号：US13074843

申请日：2011-03-29

申请人： Yehudit Natan

发明人： Yehudit Natan

IPC分类号： A01N1/02 ,  C12N5/078

CPC分类号： A61L2/0052 ,  A01N1/02 ,  A01N1/0221 ,  A61L2202/22

摘要： Provided is a preservation solution for preserving biological material at low temperature including one or more polyphenols and a method for preservation of biological material, the method includes adding the preservation solution to biological material, cooling the biological material and storing it under appropriate storing conditions. The present method may be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, and may be used with any biological material, including cells selected from RBC, WBC, MNC, UCB MNC and bacteria. In the case of RBC, also disclosed is a method for its freezing such that upon thawing, the material has less than 2% free hemoglobin.

摘要翻译： 本发明提供一种保存低温生物材料的保存液，包括一种或多种多酚和生物材料的保存方法，该方法包括将保存液添加到生物材料中，冷却生物材料并在合适的储存条件下储存。 本方法可用于低温保存或用于低温保存，包括冷冻和冻干，并且可用于任何生物材料，包括选自RBC，WBC，MNC，UCB MNC和细菌的细胞。 在RBC的情况下，还公开了其冷冻的方法，使得在解冻时，该材料具有少于2％的游离血红蛋白。

公开(公告)号：US20120276581A1

公开(公告)日：2012-11-01

申请号：US13520721

申请日：2011-01-06

申请人： Amir Arav ,  Victor Rzepakovsky ,  Isachar Norman ,  Olga Shneerson ,  Yehudit Natan

发明人： Amir Arav ,  Victor Rzepakovsky ,  Isachar Norman ,  Olga Shneerson ,  Yehudit Natan

IPC分类号： C12N5/077 ,  C12Q1/04

CPC分类号： A01N1/0221 ,  A01N1/0284 ,  A01N1/0289

摘要： The claimed subject matter is based on the finding that it is possible to cryopreserve cancellous bone tissue with viable cells (after reconstitution by either thawing or rehydration), the viability after reconstitution being comparable to that of a fresh sample (without freezing) and of beneficial value for use in, for example, transplantation. Thus, provided is a method for cryopreserving a cancellous bone sample. The cryopreserved cancellous bone sample can be in dry form, for example, lyophilized. Also provided is a cryopreserved, in dry form, bone sample and the use of a cryopreserved bone sample; a method for identifying such cryopreserved cancellous bone tissue and uses thereof.

摘要翻译： 所要求保护的主题是基于以下发现：有可能用活细胞冷冻保存松质骨组织（在通过解冻或再水合重组后），重建后的存活力与新鲜样品（不冷冻）相当并且是有益的 用于例如移植的价值。 因此，提供了一种用于冷冻保存松质骨样品的方法。 冷冻保存的松质骨样品可以是干燥形式，例如冻干的。 还提供了一种冷冻保存的干燥形式的骨样品和使用低温保存的骨样品; 用于鉴定这种冷冻保藏的松质骨组织的方法及其用途。