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公开(公告)号：US07888136B2
公开(公告)日：2011-02-15
申请号：US12099020
申请日：2008-04-07
Applicant: Stephen Yue , Ching-Ying Cheung
Inventor： Stephen Yue , Ching-Ying Cheung
CPC classification number: C12Q1/6816 , Y10S436/80 , C12Q2563/173
Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.
Abstract translation: 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。
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公开(公告)号：US20080039630A1
公开(公告)日：2008-02-14
申请号：US11782244
申请日：2007-07-24
Applicant: Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
Inventor： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
IPC: C07D401/10 , C07D209/18 , C07D403/10 , C07D403/14 , C07D417/10
CPC classification number: C12Q1/6876 , C07D209/12 , C07D209/14 , C07D209/24 , C07D401/04 , C09B23/12
Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
Abstract translation: 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。
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公开(公告)号：US20050208534A1
公开(公告)日：2005-09-22
申请号：US11005861
申请日：2004-12-06
Applicant: Jason Dallwig , David Hagen , Ching-Ying Cheung , Gerald Thomas , Stephen Yue
Inventor： Jason Dallwig , David Hagen , Ching-Ying Cheung , Gerald Thomas , Stephen Yue
IPC: C07D413/02 , C07D417/02 , C07H21/04 , C12Q1/68
CPC classification number: C09B23/164 , C07D277/64 , C07D417/06 , C09B23/0008 , C09B23/04 , C09B23/06 , C09B23/107 , C12Q1/6806 , C12Q1/6876 , C12Q2600/16 , G01N33/5308 , Y10T436/143333
Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.
Abstract translation: 具有作为核酸污渍的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物：其中R 1是C 1 -C 3烷基， 烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1至C 6烷基， -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选地是烷基，芳基，杂芳基，环状或杂环部分 被烷基，氨基，氨基烷基，羧基，硝基或卤素取代; 而其余的R 3，R 4或R 5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。
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公开(公告)号：US08242251B2
公开(公告)日：2012-08-14
申请号：US13019397
申请日：2011-02-02
Applicant: Stephen Yue , Ching-Ying Cheung
Inventor： Stephen Yue , Ching-Ying Cheung
CPC classification number: C12Q1/6816 , Y10S436/80 , C12Q2563/173
Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.
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公开(公告)号：US20080199875A1
公开(公告)日：2008-08-21
申请号：US12099020
申请日：2008-04-07
Applicant: Stephen Yue , Ching-Ying Cheung
Inventor： Stephen Yue , Ching-Ying Cheung
IPC: C12Q1/68 , C07D401/14 , C07H21/00 , G01N21/76
CPC classification number: C12Q1/6816 , Y10S436/80 , C12Q2563/173
Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.
Abstract translation: 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。
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Patent Agency Ranking
46.发明申请Flourescent chemical compounds having high selectivity for double standard DNA, and methods for their use 有权对双标准DNA具有高选择性的荧光化合物及其使用方法公开(公告)号：US20060263844A1
公开(公告)日：2006-11-23
申请号：US11432814
申请日：2006-05-11
Applicant: Jolene Bradford , Ching-Ying Cheung , Shih-Jung Huang , Patrick Pinson , Stephen Yue
Inventor： Jolene Bradford , Ching-Ying Cheung , Shih-Jung Huang , Patrick Pinson , Stephen Yue
IPC: C12Q1/02
CPC classification number: G01N33/582 , C07D215/10 , C07D215/227 , C07D277/72 , C07D277/74 , C07D417/04 , C07D417/06 , C07D417/14 , C12Q1/6876 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2201/06113 , Y10T436/143333
Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。
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47.发明申请公开(公告)号：US20060199242A1
公开(公告)日：2006-09-07
申请号：US11366784
申请日：2006-03-01
Applicant: Ching-Ying Cheung , Diane Gray , Stephen Yue
Inventor： Ching-Ying Cheung , Diane Gray , Stephen Yue
IPC: C12Q1/18 , G01N33/18 , C07D417/02
CPC classification number: G01N33/1806 , C07D211/62 , C07D295/112 , C07D401/14 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/04 , C12Q1/18 , G01N21/643 , G01N33/52 , G01N33/582 , G01N2021/6432
Abstract: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.
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公开(公告)号：US20050191643A1
公开(公告)日：2005-09-01
申请号：US10916822
申请日：2004-08-11
Applicant: Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
Inventor： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
IPC: C07D209/12 , C07D209/24 , C07D401/04 , C07D471/02 , C07D491/02 , C07D498/02 , C12Q1/68
CPC classification number: C12Q1/6876 , C07D209/12 , C07D209/14 , C07D209/24 , C07D401/04 , C09B23/12
Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
Abstract translation: 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。
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49.发明申请Fluorescent Chemical Compounds Having High Selectivity for Double Stranded DNA, and Methods for Their Use 审中-公开对双链DNA具有高选择性的荧光化学化合物及其使用方法公开(公告)号：US20110217699A1
公开(公告)日：2011-09-08
申请号：US13104413
申请日：2011-05-10
Applicant: Jolene A. Bradford , Ching-Ying Cheung , Shih Jung Huang , Patrick R. Pinson , Stephen T. Yue
Inventor： Jolene A. Bradford , Ching-Ying Cheung , Shih Jung Huang , Patrick R. Pinson , Stephen T. Yue
IPC: C12Q1/68 , C07D417/06 , C07D417/14
CPC classification number: G01N33/582 , C07D215/10 , C07D215/227 , C07D277/72 , C07D277/74 , C07D417/04 , C07D417/06 , C07D417/14 , C12Q1/6876 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2201/06113 , Y10T436/143333
Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。
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50.发明申请CHEMICAL PROBE COMPOUNDS THAT BECOME FLUORESCENT UPON REDUCTION, AND METHODS FOR THEIR USE 审中-公开化学探针化合物在使用后可能会造成荧光，以及使用方法公开(公告)号：US20080254501A1
公开(公告)日：2008-10-16
申请号：US11870238
申请日：2007-10-10
Applicant: Ching-Ying Cheung , Diane R. Gray , Stephen T. Yue
Inventor： Ching-Ying Cheung , Diane R. Gray , Stephen T. Yue
IPC: C12Q1/28 , G01N21/76 , C07D417/06 , C07D417/14 , C12Q1/02
CPC classification number: G01N33/1806 , C07D211/62 , C07D295/112 , C07D401/14 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/04 , C12Q1/18 , G01N21/643 , G01N33/52 , G01N33/582 , G01N2021/6432
Abstract: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.
Abstract translation: 公开了含有荧光团和可还原淬火单元的化学染料化合物。 可还原淬火单元在处于氧化状态时淬灭荧光团。 在还原时，淬火单元的淬火特性减弱或消除。 化合物可用于各种应用，包括检测细菌细胞，监测细菌细胞的电子传递链功能，监测非生物系统的氧化状态，以及测定抗菌剂或抗菌剂的有效性。
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