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公开(公告)号：US5141869A
公开(公告)日：1992-08-25
申请号：US544764
申请日：1990-06-27
申请人： John W. Steele , Frederick Sribnik
发明人： John W. Steele , Frederick Sribnik
CPC分类号： C12Q1/04 , C12Q1/66 , C12Q2304/60
摘要： The combination of a culturing process, bioluminescence mediated by luciferin/luciferase, and a light measuring device allows microbial monitoring in liquids with microbial concentrations as low as 1 CFU/100 ml. All characteristics of this monitor are zero gravity compatible which makes it particularly suitable for applications such as monitoring microbial counts in water in a zero gravity, closed environment.
摘要翻译： 培养过程，荧光素/荧光素酶介导的生物发光和光测量装置的组合允许在微生物浓度低至1CFU / 100ml的液体中进行微生物监测。 该显示器的所有特性都与零重力兼容，使其特别适用于在零重力，封闭环境中监测水中的微生物计数等应用。
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公开(公告)号：US20240229100A1
公开(公告)日：2024-07-11
申请号：US18411772
申请日：2024-01-12
IPC分类号： C12Q1/10 , A61K35/76 , C12N7/00 , C12N15/65 , C12N15/74 , C12N15/86 , C12Q1/02 , C12Q1/66 , C12Q1/6897 , C12Q1/70 , G01N33/50
CPC分类号： C12Q1/10 , A61K35/76 , C12N7/00 , C12N15/65 , C12N15/74 , C12N15/86 , C12Q1/025 , C12Q1/66 , C12Q1/6897 , C12Q1/70 , G01N33/50 , C12N2795/00021 , C12N2795/00031 , C12N2795/00051 , C12N2795/10131 , C12N2795/10143 , C12Q2304/60 , G01N2800/52
摘要： Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
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公开(公告)号：US20190218589A1
公开(公告)日：2019-07-18
申请号：US16247486
申请日：2019-01-14
CPC分类号： C12Q1/10 , A61K35/76 , C12N7/00 , C12N15/65 , C12N15/74 , C12N15/86 , C12N2795/00021 , C12N2795/00031 , C12N2795/00051 , C12N2795/10131 , C12N2795/10143 , C12Q1/025 , C12Q1/66 , C12Q1/6897 , C12Q1/70 , C12Q2304/60 , G01N33/50 , G01N2800/52
摘要： Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
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公开(公告)号：US20110223610A1
公开(公告)日：2011-09-15
申请号：US12588671
申请日：2009-10-23
申请人： Yoshihiro Ohmiya , Chun Wu
发明人： Yoshihiro Ohmiya , Chun Wu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/26 , C12N9/0069 , C12Q2304/60 , G01N2333/90241
摘要： The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
摘要翻译： 本发明是在不同启动子控制下的一种基因构建体或两种基因构建体或掺入Cypridina萤光素酶基因和桡足类荧光素酶的表达载体的组合。 这些基因构建体和表达载体可用于制备掺入Cypridina萤光素酶基因和桡足类荧光素酶的哺乳动物细胞，以能够在不同启动子的控制下稳定表达和细胞外分泌。
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公开(公告)号：US20080090263A1
公开(公告)日：2008-04-17
申请号：US11580501
申请日：2006-10-13
申请人： Yoshihiro Ohmiya , Chun Wu
发明人： Yoshihiro Ohmiya , Chun Wu
CPC分类号： C12Q1/26 , C12N9/0069 , C12Q2304/60 , G01N2333/90241
摘要： The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
摘要翻译： 本发明是在不同启动子控制下的一种基因构建体或两种基因构建体或掺入Cypridina萤光素酶基因和桡足类荧光素酶的表达载体的组合。 这些基因构建体和表达载体可用于制备掺入Cypridina萤光素酶基因和桡足类荧光素酶的哺乳动物细胞，以能够在不同启动子的控制下稳定表达和细胞外分泌。
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36.发明授权Method for rapidly detecting and enumerating microorganisms in mammalian cell preparations using ATP bioluminescence 有权使用ATP生物发光快速检测和列举哺乳动物细胞制剂中微生物的方法公开(公告)号：US06465201B1
公开(公告)日：2002-10-15
申请号：US09574124
申请日：2000-05-19
申请人： Esther Presente , Barbara Young , Susan Upperman
发明人： Esther Presente , Barbara Young , Susan Upperman
IPC分类号： C12Q166
CPC分类号： C12Q1/48 , C12Q1/04 , C12Q1/22 , C12Q1/66 , C12Q2304/60
摘要： A method for rapidly detecting and enumerating microorganisms, having a level of microbial ATP, in the presence of mammalian cell preparations, comprising the steps of: providing a mammalian cell preparation comprising mammalian cells having a level of mammalian ATP; reducing the level of mammalian ATP in said cell preparation by, selectively lysing the mammalian cells and not lysing microbial cells with detergent or osmotic shock to extract the mammalian ATP, treating the extracted mammalian ATP with one or more ATP hydrolyzing compounds; and immobilizing the microorganisms and washing away said detergent and the hydrolyzing compound by filtering the mammalian cell preparation through a micropartioned hydrophilic/hydrophobic membrane; extracting the microbial ATP using an extracting reagent; drying the membrane; applying a bioluminescent reagent onto the membrane; and detecting and enumerating the microorganisms.
摘要翻译： 一种用于在存在哺乳动物细胞制剂的情况下快速检测和列举具有微生物ATP水平的微生物的方法，包括以下步骤：提供包含哺乳动物ATP水平的哺乳动物细胞的哺乳动物细胞制剂; 通过选择性地裂解哺乳动物细胞并且不用洗涤剂或渗透压休克裂解微生物细胞以提取哺乳动物ATP，用一种或多种ATP水解化合物处理提取的哺乳动物ATP，从而降低所述细胞制剂中哺乳动物ATP的水平; 并通过微粒亲水/疏水膜过滤哺乳动物细胞制剂，固定微生物并洗去去除所述洗涤剂和水解化合物; 使用提取试剂提取微生物ATP; 干膜; 将生物发光试剂施加到膜上; 并检测并列举微生物。
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公开(公告)号：US06238857B1
公开(公告)日：2001-05-29
申请号：US09555682
申请日：2000-06-02
IPC分类号： C12Q100
CPC分类号： G01N33/66 , C12Q1/66 , C12Q2304/60 , G01N2400/16 , G01N2400/18
摘要： A method for analyzing an intracellular component comprising the following steps, and a reagent kit comprising (a) an extraction reagent, (b) branched dextrin or a derivative thereof, and (c) a reagent for analyzing an intracellular component: (1) step of adding an extraction reagent to a sample containing cells to extract the intracellular component; (2) step of adding branched dextrin or a derivative thereof to the sample containing the extraction reagent; and (3) step of analyzing the extracted intracellular component.
摘要翻译： 一种分析细胞内成分的方法，包括以下步骤，以及试剂盒，其包含（a）提取试剂，（b）支链糊精或其衍生物，和（c）用于分析细胞内成分的试剂：（1） 向含有细胞的样品中添加提取试剂以提取细胞内成分;（2）将含有支链糊精或其衍生物的支链糊精或其衍生物加入到含有提取试剂的样品中的步骤; 和（3）分析提取的细胞内成分的步骤。
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公开(公告)号：US5766868A
公开(公告)日：1998-06-16
申请号：US443654
申请日：1995-05-18
申请人： Susumu Seto
发明人： Susumu Seto
CPC分类号： C12Q1/04 , C12Q1/06 , C12Q2304/60 , Y10T436/25375
摘要： An improved method of determining a viable microbial cell count in a sample solution with an enhanced detection sensitivity is provided, comprising filtering the sample solution through a filtration membrane having hydrophobic properties to entrap microbes within hydrophobic barriers; applying thereto a fine spray of ATP releasing reagent to extract a luminescent ingredient from the microbes; applying thereto another fine spray of liquid luminescence-inducing reagent to allow the released luminescent ingredient to emit luminescence; and measuring the level of the luminescence, using a competent means for measuring the luminescence level. The microbes and the two types of reagents are locally concentrated due to the hydrophobic properties of the membrane and thus the sensitivity is greatly enhanced, permitting instant counting in the case of large microbes, and decreased culture time for smaller bacterial microbes.
摘要翻译： 提供了一种改进的检测灵敏度的样品溶液中微生物细菌数量确定方法，包括通过具有疏水性的过滤膜过滤样品溶液，以将微生物捕获在疏水屏障内; 向其施加细微的ATP释放试剂以从微生物提取发光成分; 施加另一种精细喷雾的液体发光诱导试剂以允许释放的发光成分发射发光; 并且使用用于测量发光水平的能力手段来测量发光的水平。 微生物和两种类型的试剂由于膜的疏水性而局部浓缩，因此敏感性大大提高，在大的微生物的情况下允许即时计数，并且减少较小细菌微生物的培养时间。
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公开(公告)号：US5624810A
公开(公告)日：1997-04-29
申请号：US370093
申请日：1995-01-09
申请人： C. David Miller , Lawrence Loomis
发明人： C. David Miller , Lawrence Loomis
IPC分类号： C12Q1/04 , G01N1/02 , G01N33/543 , G01N33/569 , C12Q1/66
CPC分类号： G01N33/56911 , C12Q1/04 , G01N33/54366 , C12Q2304/60 , G01N2001/028 , Y10S435/968
摘要： A method for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test device which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a bacterial releasing reagent and a luminescent reagent to the disposable test device and introducing the disposable test device into a luminometer that can read the luminescence from the side wall. The presence and concentration of specific microbial species is determined by adding an aliquot of the fluid phase described above to the dispoable test device, washing the sample, then adding a specific labeled antibody directed against the microbes to be detected to the test device, washing then adding a luminescent reagent to the disposable test device. The test device is then introduced into the luminometer. The relative light units determine the presence and quanity of microbes present. In both cases, the microbe is identified and/or the concentration is determined in less than 1 hour and generally in less than 5 minutes.
摘要翻译： 描述了确定总微生物污染物的存在和浓度或表面上特定微生物物种的存在和浓度的方法。 该方法包括收集装置和用于从表面除去微生物并将其悬浮在流体相中的流体的装置。 将流体相的等分试样引入一次性试验装置，其允许样品过滤以除去包括体细胞的外来物质和微生物的浓度。 微生物的总浓度通过向一次性测试装置中加入细菌释放试剂和发光试剂来测定，并将一次性测试装置引入可从侧壁读取发光的发光计。 特定微生物种类的存在和浓度通过将上述流体相的等分试样添加到可排除的测试装置中，洗涤样品，然后将针对要检测的微生物的特异性标记抗体加入到测试装置中，然后洗涤 向一次性测试装置中加入发光试剂。 然后将测试装置引入发光计。 相对光单位决定了存在的微生物的存在和质量。 在这两种情况下，确定微生物和/或浓度在少于1小时内，通常在少于5分钟内确定。
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公开(公告)号：US5416002A
公开(公告)日：1995-05-16
申请号：US984750
申请日：1992-12-03
申请人： John W. Steele , W. Clark Dean
发明人： John W. Steele , W. Clark Dean
CPC分类号： C12Q1/04 , G01N21/763 , C12Q2304/60
摘要： The use of bioluminescence on a filtration enrichment sample and a light measuring device allows microbial monitoring in liquids without sample incubation. All characteristics of this monitor are zero gravity compatible which makes it particularly suitable for applications such as monitoring microbial counts in water in a zero gravity, closed environment.
摘要翻译： 在过滤浓缩样品和光测量装置上使用生物发光使得微生物在液体中监测，无需样品孵育。 该显示器的所有特性都与零重力兼容，使其特别适用于在零重力，封闭环境中监测水中的微生物计数等应用。
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