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    Method for detection of contaminants
    1.
    发明授权
    Method for detection of contaminants 失效
    检测污染物的方法

    公开(公告)号：US5736351A

    公开(公告)日：1998-04-07

    申请号：US785833

    申请日：1997-01-15

    申请人： C. David Miller ,  Lawrence Loomis

    发明人： C. David Miller ,  Lawrence Loomis

    IPC分类号： G01N33/48 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/06 ,  G01N1/02 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/66

    CPC分类号： C12Q1/04 ,  C12Q1/06 ,  G01N33/54366 ,  G01N33/56911 ,  C12Q2304/60 ,  G01N2001/028 ,  Y10S435/968

    摘要： A method and device for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test ticket which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a somatic and bacterial releasing reagent to a disposable test device which comprises a membrane containing the luminescent reagents luciferin and luciferase, and introducing the disposable test device into a luminometer that can read the luminescence from the underside.

    摘要翻译： 描述了用于确定总微生物污染物的存在和浓度或表面上特定微生物物种的存在和浓度的方法和装置。 该方法包括收集装置和用于从表面除去微生物并将其悬浮在流体相中的流体的装置。 流体相的一部分被引入一次性检验票，其允许样品的过滤以除去包括体细胞的外来物质和微生物的浓度。 微生物的总浓度通过将体细菌和细菌释放试剂加入到一次性试验装置中来确定，所述试验装置包括含有发光试剂荧光素和荧光素酶的膜，并将一次性试验装置引入可从底侧读出发光的发光计。

    Method for detection of surfaces contaminants
    2.
    发明授权
    Method for detection of surfaces contaminants 失效
    检测表面污染物的方法

    公开(公告)号：US5624810A

    公开(公告)日：1997-04-29

    申请号：US370093

    申请日：1995-01-09

    申请人： C. David Miller ,  Lawrence Loomis

    发明人： C. David Miller ,  Lawrence Loomis

    IPC分类号： C12Q1/04 ,  G01N1/02 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/66

    CPC分类号： G01N33/56911 ,  C12Q1/04 ,  G01N33/54366 ,  C12Q2304/60 ,  G01N2001/028 ,  Y10S435/968

    摘要： A method for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test device which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a bacterial releasing reagent and a luminescent reagent to the disposable test device and introducing the disposable test device into a luminometer that can read the luminescence from the side wall. The presence and concentration of specific microbial species is determined by adding an aliquot of the fluid phase described above to the dispoable test device, washing the sample, then adding a specific labeled antibody directed against the microbes to be detected to the test device, washing then adding a luminescent reagent to the disposable test device. The test device is then introduced into the luminometer. The relative light units determine the presence and quanity of microbes present. In both cases, the microbe is identified and/or the concentration is determined in less than 1 hour and generally in less than 5 minutes.

    摘要翻译： 描述了确定总微生物污染物的存在和浓度或表面上特定微生物物种的存在和浓度的方法。 该方法包括收集装置和用于从表面除去微生物并将其悬浮在流体相中的流体的装置。 将流体相的等分试样引入一次性试验装置，其允许样品过滤以除去包括体细胞的外来物质和微生物的浓度。 微生物的总浓度通过向一次性测试装置中加入细菌释放试剂和发光试剂来测定，并将一次性测试装置引入可从侧壁读取发光的发光计。 特定微生物种类的存在和浓度通过将上述流体相的等分试样添加到可排除的测试装置中，洗涤样品，然后将针对要检测的微生物的特异性标记抗体加入到测试装置中，然后洗涤 向一次性测试装置中加入发光试剂。 然后将测试装置引入发光计。 相对光单位决定了存在的微生物的存在和质量。 在这两种情况下，确定微生物和/或浓度在少于1小时内，通常在少于5分钟内确定。

    Method of treating upper resiratory illnesses
    4.
    发明授权
    Method of treating upper resiratory illnesses 失效
    治疗上呼吸道疾病的方法

    公开(公告)号：US06752988B1

    公开(公告)日：2004-06-22

    申请号：US09560650

    申请日：2000-04-28

    申请人： Vincent Fischetti ,  Lawrence Loomis

    发明人： Vincent Fischetti ,  Lawrence Loomis

    IPC分类号： A61K3843

    CPC分类号： A61K38/162 ,  A61K38/47 ,  Y02A50/473 ,  Y02A50/481 ,  A61K2300/00

    摘要： A method for the prophylactic and therapeutic treatment of bacterial infections is disclosed which comprises the treatment of an individual with an effective amount of a native or modified version of a lytic enzyme produced by a bacteria infected with a bacteriophage specific for said bacteria. The lytic enzyme is selected from a group consisting of shuffled lytic enzymes, chimericlytic enzymes, wherein the lytic enzyme is in an environment having a pH which allows for activity of said lytic enzyme; and a carrier for delivering said lytic enzyme. Additionally, a holin enzyme for puncturing the membrane may be included in the composition. This method, and composition can be used for the treatment of upper respiratory infections, skin infections, wounds, and burns, vaginal infections, eye infections, intestinal disorders and dental problems.

    摘要翻译： 公开了一种用于预防和治疗细菌感染的方法，其包括用有效量的由所述细菌特异性噬菌体感染的细菌产生的裂解酶的天然或修饰形式处理个体。 溶解酶选自洗牌溶解酶，嵌合分解酶，其中溶解酶在具有允许所述溶解酶活性的pH的环境中; 和用于递送所述裂解酶的载体。 另外，用于穿刺膜的胃蛋白酶可以包括在组合物中。 该方法和组合物可用于治疗上呼吸道感染，皮肤感染，伤口和烧伤，阴道感染，眼睛感染，肠道疾病和牙齿问题。

    Use of bacterial phage associated lysing enzymes for treating streptococcal infections of the upper respiratory tract
    5.
    发明授权
    Use of bacterial phage associated lysing enzymes for treating streptococcal infections of the upper respiratory tract 有权
    使用细菌噬菌体相关的裂解酶治疗上呼吸道的链球菌感染

    公开(公告)号：US06326002B1

    公开(公告)日：2001-12-04

    申请号：US09671990

    申请日：2000-09-28

    申请人： Vincent Fischetti ,  Lawrence Loomis

    发明人： Vincent Fischetti ,  Lawrence Loomis

    IPC分类号： A61K3800

    摘要： The present invention discloses a method for treating Streptococcal infections of the upper respiratory tract, comprising administering an effective amount of a lysin enzyme produced by group C Streptococcal bacteria infected with a C1 bacteriophage specific for the bacteria to a mouth, throat, or nasal passage of a mammal, the method providing a concentration of from about 100 to 500,000 active enzyme units per milliliter of fluid in the environment of said nasal or oral passages.

    摘要翻译： 本发明公开了一种治疗上呼吸道链球菌感染的方法，其特征在于，将由感染了细菌特异性的C1噬菌体的C组链球菌细菌产生的有效量的赖氨酸酶给予口腔，咽喉或鼻腔通道 哺乳动物，该方法在所述鼻腔或口腔通道的环境中提供浓度为约100至500,000活性酶单位/毫升流体。

    Rapid analyte collection and testing device
    7.
    发明申请
    Rapid analyte collection and testing device 审中-公开
    快速分析物收集和测试装置

    公开(公告)号：US20130068040A1

    公开(公告)日：2013-03-21

    申请号：US13507906

    申请日：2012-08-06

    申请人： Lawrence Loomis ,  Leslie Kirkegaard ,  Glen Ford ,  Robert Greenfield

    发明人： Lawrence Loomis ,  Leslie Kirkegaard ,  Glen Ford ,  Robert Greenfield

    IPC分类号： G01N1/10

    CPC分类号： G01N1/10 ,  G01N1/02 ,  G01N1/38 ,  G01N2001/028 ,  G01N2001/383

    摘要： A rapid analyte collection and testing device, said device comprising a) a casing, said casing having) a first casing section, said first casing section containing an encapsulated buffer section containing a buffer, and asecond casing section, said second casing section comprising a window on a side of said second section, a complementary mechanism for attachment to said first casing section, an opening at a proximal end of said second casing section; and a non permeable platform strip positioned lengthwise within and extending beyond the second casing section. The non-permeable platform further contains a swab, said swab positioned at the distal end of said non-permeable platform strip, a lateral flow assay positioned downstream from said swab, and a tag, positioned upstream from the capture reagent site.

    摘要翻译： 一种快速的分析物收集和测试装置，所述装置包括：a）壳体，所述壳体具有第一壳体部分，所述第一壳体部分包含含有缓冲器的封装缓冲部分，以及第二壳体部分，所述第二壳体部分包括窗口 在所述第二部分的一侧，具有用于附接到所述第一壳体部分的互补机构，在所述第二壳体部分的近端处的开口; 以及不可渗透的平台条，其长度方向定位在第二壳体部分内并延伸超过第二壳体部分。 所述非透过性平台还包含拭子，所述拭子位于所述不可渗透平台条的远端，位于所述拭子下游的侧向流动测定装置和位于捕获试剂部位上游的标签。

    Use of bacterial phage associated lysing enzymes for treating various illnesses
    9.
    发明申请
    Use of bacterial phage associated lysing enzymes for treating various illnesses 审中-公开
    使用细菌噬菌体相关的裂解酶治疗各种疾病

    公开(公告)号：US20050136088A1

    公开(公告)日：2005-06-23

    申请号：US10855978

    申请日：2004-05-28

    申请人： Lawrence Loomis ,  Vincent Fischetti

    发明人： Lawrence Loomis ,  Vincent Fischetti

    IPC分类号： A61K38/46 ,  A61K38/43 ,  A61K9/00

    CPC分类号： A61K38/162 ,  A61K38/47 ,  Y02A50/473 ,  Y02A50/481 ,  A61K2300/00

    摘要： A composition and method for treating bacterial infections is disclosed which comprises the treatment of an individual with an effective amount of at least one lytic enzyme produced by a bacteria infected with a bacteriophage specific for said bacteria wherein at least one lytic enzyme is selected from the group consisting of shuffled lytic enzymes, chimeric lytic enzymes, holin enzymes, and combinations thereof. A carrier may be used for delivering the lytic enzyme. This method, and composition can be used for the treatment of upper respiratory infections, skin infections, wounds, and burns, vaginal infections, eye infections, intestinal disorders and dental problems.

    摘要翻译： 公开了一种用于治疗细菌感染的组合物和方法，其包括用有效量的至少一种裂解酶处理个体，所述裂解酶由感染了所述细菌特异性噬菌体的细菌产生，其中至少一种溶解酶选自 由洗牌的裂解酶，嵌合裂解酶，胃蛋白酶及其组合组成。 载体可用于递送溶解酶。 该方法和组合物可用于治疗上呼吸道感染，皮肤感染，伤口和烧伤，阴道感染，眼睛感染，肠道疾病和牙齿问题。