公开(公告)号：US5736351A

公开(公告)日：1998-04-07

申请号：US785833

申请日：1997-01-15

申请人： C. David Miller ,  Lawrence Loomis

发明人： C. David Miller ,  Lawrence Loomis

IPC分类号： G01N33/48 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/06 ,  G01N1/02 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/66

CPC分类号： C12Q1/04 ,  C12Q1/06 ,  G01N33/54366 ,  G01N33/56911 ,  C12Q2304/60 ,  G01N2001/028 ,  Y10S435/968

摘要： A method and device for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test ticket which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a somatic and bacterial releasing reagent to a disposable test device which comprises a membrane containing the luminescent reagents luciferin and luciferase, and introducing the disposable test device into a luminometer that can read the luminescence from the underside.

摘要翻译： 描述了用于确定总微生物污染物的存在和浓度或表面上特定微生物物种的存在和浓度的方法和装置。 该方法包括收集装置和用于从表面除去微生物并将其悬浮在流体相中的流体的装置。 流体相的一部分被引入一次性检验票，其允许样品的过滤以除去包括体细胞的外来物质和微生物的浓度。 微生物的总浓度通过将体细菌和细菌释放试剂加入到一次性试验装置中来确定，所述试验装置包括含有发光试剂荧光素和荧光素酶的膜，并将一次性试验装置引入可从底侧读出发光的发光计。

公开(公告)号：US5624810A

公开(公告)日：1997-04-29

申请号：US370093

申请日：1995-01-09

申请人： C. David Miller ,  Lawrence Loomis

发明人： C. David Miller ,  Lawrence Loomis

IPC分类号： C12Q1/04 ,  G01N1/02 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/66

CPC分类号： G01N33/56911 ,  C12Q1/04 ,  G01N33/54366 ,  C12Q2304/60 ,  G01N2001/028 ,  Y10S435/968

摘要： A method for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test device which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a bacterial releasing reagent and a luminescent reagent to the disposable test device and introducing the disposable test device into a luminometer that can read the luminescence from the side wall. The presence and concentration of specific microbial species is determined by adding an aliquot of the fluid phase described above to the dispoable test device, washing the sample, then adding a specific labeled antibody directed against the microbes to be detected to the test device, washing then adding a luminescent reagent to the disposable test device. The test device is then introduced into the luminometer. The relative light units determine the presence and quanity of microbes present. In both cases, the microbe is identified and/or the concentration is determined in less than 1 hour and generally in less than 5 minutes.

摘要翻译： 描述了确定总微生物污染物的存在和浓度或表面上特定微生物物种的存在和浓度的方法。 该方法包括收集装置和用于从表面除去微生物并将其悬浮在流体相中的流体的装置。 将流体相的等分试样引入一次性试验装置，其允许样品过滤以除去包括体细胞的外来物质和微生物的浓度。 微生物的总浓度通过向一次性测试装置中加入细菌释放试剂和发光试剂来测定，并将一次性测试装置引入可从侧壁读取发光的发光计。 特定微生物种类的存在和浓度通过将上述流体相的等分试样添加到可排除的测试装置中，洗涤样品，然后将针对要检测的微生物的特异性标记抗体加入到测试装置中，然后洗涤 向一次性测试装置中加入发光试剂。 然后将测试装置引入发光计。 相对光单位决定了存在的微生物的存在和质量。 在这两种情况下，确定微生物和/或浓度在少于1小时内，通常在少于5分钟内确定。

公开(公告)号：US6146591A

公开(公告)日：2000-11-14

申请号：US70737

申请日：1998-05-01

申请人： C David Miller

发明人： C David Miller

IPC分类号： G01N1/22 ,  G01N21/25 ,  G01N35/02 ,  G01N35/04 ,  G01N21/00

CPC分类号： G01N21/253 ,  G01N35/028 ,  G01N2001/2217 ,  G01N2035/0436 ,  Y10T436/110833 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： This device discloses a portable sequential contaminant detection system which can test samples for contaminants at predetermined timed intervals. The detection system has a motor to advance a matrix, a motor to raise and lower syringes holding liquefied samples of air to be tested, and a motor to raise and lower plungers within the syringes to draw up fluids or expel fluids into reservoir wells of the matrix to test air or other samples for contaminants. The system relies upon a "matrix" which will hold reagents with which the air samples will be mixed. The matrix is stepped to move into precise registration with the "read" module where the relative reflectance of each capture membrane reads statically by a fixed LED, silicon photodiode and amplifier associated with each channel.

摘要翻译： 该装置公开了一种便携式顺序污染物检测系统，其可以以预定的时间间隔测试样品的污染物。 检测系统具有马达推进矩阵，马达升高和降低保持要测试的空气的液化样品的注射器，以及用于升高和降低注射器内的柱塞的马达，以便将流体排出流体或将流体排出到 用于测试空气或其他样品的污染物。 该系统依赖于“基质”，其将容纳将与空气样品混合的试剂。 矩阵被逐步移动到与“读取”模块的精确配准，其中每个捕获膜的相对反射率由与每个通道相关联的固定LED，硅光电二极管和放大器静态地读取。