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    Carbohydrate-Binding Modules of a New Family
    32.
    发明申请
    Carbohydrate-Binding Modules of a New Family 审中-公开
    新家庭的碳水化合物绑定模块

    公开(公告)号：US20150024465A1

    公开(公告)日：2015-01-22

    申请号：US14500564

    申请日：2014-09-29

    申请人： Novozymes A/S

    发明人： Kirk Matthew Schnorr ,  Lars Lehmann Hylling Christensen

    IPC分类号： C12N9/42 ,  C07K14/37

    CPC分类号： C12N9/2437 ,  C07K14/37 ,  C12N9/2434

    摘要： The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.

    摘要翻译： 本发明涉及属于CBM新家族的非催化碳水化合物结合模块（CBM）。 发现本发明的CBM附着于糖基水解酶家族61（GH61）多肽，并且与已知的CBM具有很小的同源性，表明它是CBM新家族的第一个已知成员。 本发明还涉及CBM优选表现出对纤维素的结合亲和性; 一种生产这种煤层气的方法; 以及在织物，洗涤剂和纤维素纤维加工工业中使用这种CBM的方法，用于纯化多肽，活化酶的固定化，烘焙，制造生物燃料，修饰植物细胞壁。

    Integrated process system for single cell oil production and a pulp and/or paper industry process
    33.
    发明授权
    Integrated process system for single cell oil production and a pulp and/or paper industry process 有权
    用于单细胞油生产的综合过程系统和纸浆和/或造纸工业过程

    公开(公告)号：US08936655B2

    公开(公告)日：2015-01-20

    申请号：US13333203

    申请日：2011-12-21

    申请人： Perttu Koskinen ,  Reijo Tanner

    发明人： Perttu Koskinen ,  Reijo Tanner

    IPC分类号： C10L1/00 ,  C12P7/64 ,  C12N9/42 ,  C12P21/02 ,  D21C5/00 ,  D21H11/20

    CPC分类号： C12P7/64 ,  C12N9/2434 ,  C12P21/02 ,  D21C5/005 ,  D21H11/20 ,  Y02E50/13 ,  Y02P20/125 ,  Y02P20/582 ,  Y02T50/678

    摘要： The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.

    摘要翻译： 本发明涉及一种综合方法，其包括单细胞油生产过程和纸浆和/或造纸工业方法。 该方法包括在单细胞油生产过程中使用能够在包含来自纸浆和/或造纸工业的有机材料的培养基上培养时能够产生脂质或脂质和酶的微生物。 在单细胞油生产过程中和/或与其连接的过程中，所述微生物产生脂质或脂质和酶。 本发明还涉及在该方法中生产的脂质作为生物燃料或作为生物燃料的组分或用作生物燃料生产和使用在纸浆和/或造纸工业或其它应用中的脂质生产过程中生产的酶的原料 作为酶制剂或酶的来源。

    METHODS OF INCREASING SECRETION OF POLYPEPTIDES HAVING BIOLOGICAL ACTIVITY
    38.
    发明申请
    METHODS OF INCREASING SECRETION OF POLYPEPTIDES HAVING BIOLOGICAL ACTIVITY 审中-公开
    增加生物活性多肽分泌的方法

    公开(公告)号：US20140248671A1

    公开(公告)日：2014-09-04

    申请号：US14279624

    申请日：2014-05-16

    申请人： Novozymes, Inc.

    发明人： Sandra Merino

    IPC分类号： C12P19/14 ,  C12P19/02

    CPC分类号： C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

    摘要： The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

    摘要翻译： 本发明涉及生产具有生物活性的分泌多肽的方法，其包括：（a）用编码融合蛋白的融合蛋白构建体转化真菌宿主细胞，其包含：（i）编码信号肽的第一多核苷酸; （ii）至少编码内切葡聚糖酶或其部分的催化结构域的第二多核苷酸; 和（iii）至少编码具有生物活性的多肽的催化结构域的第三个多核苷酸; 其中所述信号肽和至少所述内切葡聚糖酶的催化结构域与至少所述内切葡聚糖酶的催化结构域相比，增加具有生物活性的多肽的分泌; （b）在适合于产生融合蛋白的条件下培养转化的真菌宿主细胞; 和（c）从培养基中回收具有生物活性的融合蛋白，其组分或其组合。