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公开(公告)号：US09644232B2
公开(公告)日：2017-05-09
申请号：US13952173
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew Grossmann , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
IPC: C12Q1/68
CPC classification number: C12N15/1017 , B01L7/52 , B01L2300/0681 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US20170035914A1
公开(公告)日：2017-02-09
申请号：US14818372
申请日：2015-08-05
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , Michael James Rishel , Andrew Arthur Paul Burns
IPC: A61K49/00
CPC classification number: A61K49/0056 , A61K49/0043
Abstract: Disclosed are novel cell-penetrating transporters for enhancing cellular uptake of peptide fusion proteins into live cells. The cell-penetrating transporters comprise a functionalized peptide construct comprising a cell importation peptide covalently bound to a cargo, the cell importation peptide in an unreactive monomeric form, and a pharmaceutical carrier. In certain embodiments, the cargo further comprises a nuclear localization sequence (NLS) allowing the cargo to be transported across the nuclear membrane.
Abstract translation: 公开了用于增强肽融合蛋白到活细胞中的细胞摄取的新型细胞穿透转运蛋白。 细胞穿透转运蛋白包含功能化的肽构建体，其包含与货物共价结合的细胞进入肽，细胞进口肽以非反应性单体形式和药物载体。 在某些实施方案中，货物还包括允许货物跨过核膜运输的核定位序列（NLS）。
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公开(公告)号：US20160053307A1
公开(公告)日：2016-02-25
申请号：US14933275
申请日：2015-11-05
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 还提供了使用连接辅助的DNA扩增在单个管中的全基因组扩增线性染色体DNA。
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公开(公告)号：US09217167B2
公开(公告)日：2015-12-22
申请号：US13952040
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Erik Leeming Kvam
CPC classification number: C12P19/34 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.
Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 提供了从血液中提取的循环核酸的全基因组扩增。 还提供了用于执行所公开的方法的套件。
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35.发明申请公开(公告)号：US20150166956A1
公开(公告)日：2015-06-18
申请号：US14167393
申请日：2014-01-29
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Erik Leeming Kvam , Gregory Andrew Grossmann , Craig Patrick Galligan , Jason Michael Nichols , Xuefeng Wang , Jason Louis Davis
IPC: C12N5/078
CPC classification number: B01D21/0087 , B01D17/02 , B01D21/0006 , B01D21/0042 , B01D21/02 , B01D21/10 , B01D21/2433 , B01D21/2444 , B01D21/245 , B01D2221/04 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2300/0681 , B01L2300/0851 , B01L2400/0421 , B01L2400/043 , B01L2400/0457 , B01L2400/0469 , C02F1/00 , C02F1/24 , C02F1/444 , C02F1/48 , C02F2001/007 , C02F2101/32 , C02F2103/08 , C02F2103/10 , C02F2201/002 , C12M47/02 , C12N5/0641
Abstract: A device is configured for separation of particulates dispersed within a base fluid, wherein the particulates have a relative density difference compared to the base fluid. The device comprises a microchannel of length l and height h comprising an inlet and an outlet; a microporous surface on one or more walls of the microchannel; a collection chamber on an opposing side of the microporous surface; and an applied force field across the height h of the microchannel to sediment the particles through the microporous surface into the collection chamber. The microporous body operationally generates a fluid flow regime comprising a first fluid flow having a first flow rate through the microchannel and a second fluid flow having a second flow rate through the collection chamber and the second flow rate is a fraction of the first flow rate.
Abstract translation: 一种装置被配置用于分离分散在基础流体中的微粒，其中所述颗粒与基础流体相比具有相对密度差。 该装置包括长度为l的微通道和包括入口和出口的高度h; 在微通道的一个或多个壁上的微孔表面; 在微孔表面的相对侧上的收集室; 以及穿过微通道的高度h的施加的力场，以将颗粒通过微孔表面沉淀到收集室中。 微孔体可操作地产生流体流动状态，其包括具有通过微通道的第一流量的第一流体流和具有通过收集室的第二流速的第二流体流，第二流量是第一流量的一部分。
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Patent Agency Ranking
公开(公告)号：US08981069B2
公开(公告)日：2015-03-17
申请号：US13721948
申请日：2012-12-20
Applicant: General Electric Company
Inventor： Brian Christopher Bales , Erik Leeming Kvam , Jason Louis Davis
Abstract: The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.
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公开(公告)号：US20240318225A1
公开(公告)日：2024-09-26
申请号：US18293305
申请日：2022-07-29
Inventor： Christopher Michael Puleo , Erik Leeming Kvam , Pak Kin Wong , Peter Torab , Christine Lynne Surrette
CPC classification number: C12Q1/24 , C12Q1/18 , G01N33/491
Abstract: The subject matter of the present disclosure generally relates to techniques for isolating bacterial cells from a biological sample comprising red blood cells. Using an aggregating agent and an anticoagulant during sedimentation permits separation of bacterial pathogens in the sample from red blood cells. The separated sedimentation layer, which is enriched in any bacterial pathogens, can be centrifuged and resuspended to concentrate the bacteria for additional analysis, such as bacterial identification and/or antibiotic susceptibility tests.
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公开(公告)号：US11590502B2
公开(公告)日：2023-02-28
申请号：US16459363
申请日：2019-07-01
Applicant: General Electric Company
Inventor： Alex David Corwin , Erik Leeming Kvam , Christine Lynne Surrette , Brian Joseph Scherer , Pei-Hsin Kuo , Ralf Lenigk , Tyler John Hammond
Abstract: The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.
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公开(公告)号：US20180223349A1
公开(公告)日：2018-08-09
申请号：US15949061
申请日：2018-04-09
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
IPC: C12Q1/6844 , C12Q1/6869
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/179 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
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公开(公告)号：US10000742B2
公开(公告)日：2018-06-19
申请号：US14945483
申请日：2015-11-19
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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