公开(公告)号：US20190079047A1

公开(公告)日：2019-03-14

申请号：US16101337

申请日：2018-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber

IPC: G01N27/414 ,  H01L21/82 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US09964515B2

公开(公告)日：2018-05-08

申请号：US14862930

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  James Bustillo ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US12066399B2

公开(公告)日：2024-08-20

申请号：US18353017

申请日：2023-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/78 ,  H01L29/42324 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US11874250B2

公开(公告)日：2024-01-16

申请号：US17823696

申请日：2022-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q1/6874 ,  C12Q2565/607

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11448613B2

公开(公告)日：2022-09-20

申请号：US16101337

申请日：2018-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US20200378919A1

公开(公告)日：2020-12-03

申请号：US16924055

申请日：2020-07-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark James MILGREW ,  Jonathan M. Rothberg ,  James Bustillo

IPC: G01N27/414 ,  G01N27/27 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US10605734B2

公开(公告)日：2020-03-31

申请号：US14779532

申请日：2014-04-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： William M. Lafferty ,  Jonathan M. Rothberg ,  Keith G. Fife

IPC: G01N21/64 ,  C12Q1/6874

Abstract: A device including a transparent layer defining a surface exposed to a flow volume and to secure a target polynucleotide template and a detector structure secured to the transparent layer and including a plurality of detectors to detect a signal emitted during nucleotide incorporation along the target polynucleotide template.

公开(公告)号：US10415079B2

公开(公告)日：2019-09-17

申请号：US15192225

申请日：2016-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz

IPC: C12Q1/68 ,  C12Q1/6825 ,  H01L29/78 ,  G01N27/26 ,  G01N33/68 ,  G01N27/414 ,  C12Q1/6874 ,  G01N33/543 ,  H01L29/788 ,  C12Q1/6869

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US09249461B2

公开(公告)日：2016-02-02

申请号：US14044712

申请日：2013-10-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan M. Rothberg

IPC: C12Q1/68 ,  G01N27/414 ,  B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/06

CPC classification number: B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

公开(公告)号：US08766328B2

公开(公告)日：2014-07-01

申请号：US13923282

申请日：2013-06-20

Applicant: Life Technologies Corporation

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/403

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。