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21.发明申请公开(公告)号：US20210055282A1
公开(公告)日：2021-02-25
申请号：US17091501
申请日：2020-11-06
Applicant: General Electric Company , Tokitae LLC
Inventor： Matthew Jeremiah Misner , Gregory Andrew Grossmann , Cathryn Ellen Olsen , John Richard Nelson , David Roger Moore , Paul Michael Smigelski , John Thomas Connelly , Benjamin David Grant , Bernhard Hans Weigl
IPC: G01N33/493 , C07K1/22 , B01D15/08 , G01N33/50 , G01N33/569 , B01D63/02 , A61B10/00 , C12M1/26 , G01N33/558
Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.
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公开(公告)号：US10787695B2
公开(公告)日：2020-09-29
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: C12Q1/02 , C12M1/34 , G01N21/64 , C12Q1/18 , G01N33/483
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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公开(公告)号：US10597691B2
公开(公告)日：2020-03-24
申请号：US15408509
申请日：2017-01-18
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper
IPC: C12P19/34 , C12Q1/6844
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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公开(公告)号：US20180273914A1
公开(公告)日：2018-09-27
申请号：US16005633
申请日：2018-06-11
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
IPC: C12N7/00 , C12Q1/70 , C12Q1/6806 , B01L3/00 , C12N15/10
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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公开(公告)号：US09999856B2
公开(公告)日：2018-06-19
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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Patent Agency Ranking
公开(公告)号：US20170211059A1
公开(公告)日：2017-07-27
申请号：US15463943
申请日：2017-03-20
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew GROSSMANN , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
CPC classification number: C12N15/1017 , B01L3/5023 , B01L7/52 , B01L2300/0681 , B01L2300/0825 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US20170145387A1
公开(公告)日：2017-05-25
申请号：US14945483
申请日：2015-11-19
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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公开(公告)号：US09644232B2
公开(公告)日：2017-05-09
申请号：US13952173
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew Grossmann , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
IPC: C12Q1/68
CPC classification number: C12N15/1017 , B01L7/52 , B01L2300/0681 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US20170121747A1
公开(公告)日：2017-05-04
申请号：US15408509
申请日：2017-01-18
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper
IPC: C12P19/34
CPC classification number: C12P19/34 , C12Q1/6844 , C12Q2527/107 , C12Q2527/125 , C12Q2527/137 , C12Q2531/119
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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公开(公告)号：US09625355B2
公开(公告)日：2017-04-18
申请号：US14557169
申请日：2014-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
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